Rhodamine phalloidin phalloidin tritc

Proanthocyanidin (PC), iron(III) nitrate nonahydrate (Fe(NO₃)₃∙9H₂O), N-2-hydroxyethyl piperazine-N'-2-sodium sulfonate salt (HEPES-Na), bis-(2-hydroxyethyl)amino-tris(hydroxymethyl)methane (bis–tris), silver nitrate (Ag(NO₃)₃) and tris(hydroxymethyl)aminomethane (tris) were purchased from Macklin. NH₃∙H₂O, H₂SO₄, H₂O₂ (30%), phosphate-buffered saline (PBS), 4′,6-diamidino-2-phenylindole (DAPI), rhodamine phalloidin (phalloidin-TRITC) and polypropylene (PP) sheets were purchased from Sigma. Four percent paraformaldehyde and Triton X-100 were purchased from Solarbio Life Science. Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Laboratories. The ROS assay kit and ferric reducing antioxidant power (FRAP) assay kit were purchased from Beyotime Institute of Biotechnology. All chemicals were used without any further purification. Silicon wafers (SSPs), quartz plates (Alfa Aesar), and round glass coverslips (14 mm diameter from NEST) were cleaned by piranha solution (70% H₂SO₄ and 30% H₂O₂, V/V) at 98 ℃ for 2 h, rinsed with Milli-Q water and subsequently dried under a mild stream of air before use (Caution: Piranha solution is highly oxidizing and corrosive, and extreme care should be taken during preparation and use). The deionized (DI) water used here was purified through a Milli-Q system and possessed a resistivity greater than 18.25 MΩ·cm.