Ultra test strips

Glucose levels were measured in blood collected from the tail vein using OneTouch glucometer and Ultra test strips (LifeScan, Milpitas, CA) as previously described [10 (link)–11 (link)]. Albumin and creatinine excretion was determined in spot urine collected from individually caged mice using Albuwell-M kits (Exocell Inc, Philadelphia, PA) as previously described [10 (link)–11 (link)].

Animal experiments were performed at the AAALAC-accredited animal facilities at Vanderbilt University Medical Center according to institutional guidelines and IACUC-approved experimental protocol. In this study, we employed eNOS^−/− C57BLKS db/db mice, a mouse model of Type 2 DN.^{27 (link)} Some mice were treated with pyridoxamine (PM) as previously described.^{9 (link)} Control group included wild type C57BLKS mice. Experiments continued until mice were sacrificed at 12 wks or 22 wks of age and kidneys were removed, flash frozen in liquid nitrogen and stored at −80°C. Glucose levels were measured in blood collected from the tail vein using OneTouch glucometer and Ultra test strips (LifeScan, Milpitas, CA). Albumin and creatinine excretion was determined in spot urine collected from individually caged mice using Albuwell-M kits (Exocell Inc, Philadelphia, PA).