Palm shell was provided by the Faculty of Agriculture, Cairo University, Egypt. KOH, NaOH, and FeSO4·7H2O were obtained from the Central Drug House (P) Ltd., India. K2CO3 and FeCl3 were purchased from Qualikems Fine Chem. Pvt. Ltd., India. Pb(NO3)2 and CdCl2 were purchased from LOBA Chemie, India. AgNO3, fecal coliform, and Bacillus subtilis strains were kindly provided by the Soils, Water, and Environmental Res. Inst. (ARC) in Giza, Egypt.
Pb no3 2
Manufactured by Loba Chemie
Sourced in India
Pb(NO3)2 is an inorganic compound with the chemical formula lead(II) nitrate. It is a white, crystalline solid that is soluble in water.
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Lab products found in correlation
3 protocols using pb no3 2
1
Bioremediation of Heavy Metals Using Palm Shell
2
Insulin Cytotoxicity Assay Protocol
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All the metal salts of analytical grade, including NiSO4 (nickel sulphate) and Pb(NO3)2 (lead nitrate) were purchased from Loba Chemie, India. Recombinant human insulin was purchased from (Elli Lilly, India). DMEM cell culture media, fetal bovine serum (FBS), penicillin–streptomycin were purchased from HiMedia, India. The rest of the chemicals were purchased from HiMedia and were of analytical grade.
3
Spectrophotometric Determination of Lead(II)
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All chemical reagents used in this work were of analytical grade. Deionized water was used for solution preparation.
A 1000 µg mL−1 stock solution of Pb2+ was prepared by dissolving 0.160× g of Pb(NO3)2 (Loba Chemie, Mumbai, India) in 100 mL of water in a volumetric flask. Working solutions with various concentrations of Pb2+ were prepared daily by diluting with deionized water.
The solution of 0.05% w/v of 4-(2-pyridylazo) resorcinol (PAR) at pH 7 was prepared by dissolving 0.05 g of 4-(2-pyridylazo) resorcinol (TCI, Tokyo, Japan) in 1 mol L−1 NaOH, adjusting the pH to 7 with 2 mol L−1 of nitric acid, and diluting it to 100 mL in a volumetric flask with deionized water.
A 1000 µg mL−1 stock solution of Pb2+ was prepared by dissolving 0.160× g of Pb(NO3)2 (Loba Chemie, Mumbai, India) in 100 mL of water in a volumetric flask. Working solutions with various concentrations of Pb2+ were prepared daily by diluting with deionized water.
The solution of 0.05% w/v of 4-(2-pyridylazo) resorcinol (PAR) at pH 7 was prepared by dissolving 0.05 g of 4-(2-pyridylazo) resorcinol (TCI, Tokyo, Japan) in 1 mol L−1 NaOH, adjusting the pH to 7 with 2 mol L−1 of nitric acid, and diluting it to 100 mL in a volumetric flask with deionized water.
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