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Incb3284

Manufactured by Cayman Chemical
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INCB3284 is a laboratory product manufactured by Cayman Chemical. It is a small molecule inhibitor used for research purposes.

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Lab products found in correlation

Phospho ikba ser32, by Cell Signaling Technology (2 mentions) G 6983, by Cayman Chemical (2 mentions) Cd154, by Santa Cruz Biotechnology (2 mentions) Nfatc1, by BioLegend (2 mentions) Bay11 7082, by Enzo Life Sciences (2 mentions) Recombinant human ccl2, by Thermo Fisher Scientific (1 mentions)

3 protocols using incb3284

1

Signaling Pathways in Cell Activation

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The following chemicals were obtained: Cyclosporin A (TCI Chemical cat no.C2408), Bay11–7082 (Enzo cat no.E1279), PP2 (Tocris cat no.1407), Gö 6983 (Cayman Chemical cat no.13311), INCB3284 (Cayman Chemical cat no.1163). 200,000 cells seeded in 24 well plates were treated with/without inhibitors. Protein cell lysates were prepared and immunoblotted using procedures described24 (link). Membranes were blotted with antibodies to: CD154 (Santa Cruz Biotechnology cat no. sc978), NFATc1 (Biolegend, cat no.649601), phospho-IKBa (ser32) (Cell Signaling Technology cat no. 9246), IΚBα or ACTIN (Sigma, cat no.A5441).
Brummer G., Fang W., Smart C., Zinda B., Alissa N., Berkland C., Miller D, & Cheng N. (2019). CCR2 signaling in breast carcinoma cells promotes tumor growth and invasion by promoting CCL2 and suppressing CD154 effects on the angiogenic and immune microenvironments. Oncogene, 39(11), 2275-2289.
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2

3D Culture Analysis of CCL2 and INCB3284

Cited 1 time
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3D cultures were established and analyzed as previously described27 (link). Cells were treated with/without 100 ng/ml human recombinant CCL2 (Peprotech cat no. 300-04) or 20 nM INCB3284 (Cayman Chemical cat no. 11963) in growth media containing 2.5% Matrigel. Images were captured at day 4 for hDCIS.01 cells and day 8 for SUM225 cells at 10× magnification, 4 fields/well using an EVOS FL Auto Imaging system. Spheroid size was quantified using Image J software.
Fang W.B., Sofia Acevedo D., Smart C., Zinda B., Alissa N., Warren K., Fraga G., Huang L.C., Shyr Y., Li W., Xie L., Staggs V., Hong Y., Behbod F, & Cheng N. (2021). Expression of CCL2/CCR2 signaling proteins in breast carcinoma cells is associated with invasive progression. Scientific Reports, 11, 8708.
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3

Signaling Pathways in Cell Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following chemicals were obtained: Cyclosporin A (TCI Chemical cat no.C2408), Bay11–7082 (Enzo cat no.E1279), PP2 (Tocris cat no.1407), Gö 6983 (Cayman Chemical cat no.13311), INCB3284 (Cayman Chemical cat no.1163). 200,000 cells seeded in 24 well plates were treated with/without inhibitors. Protein cell lysates were prepared and immunoblotted using procedures described24 (link). Membranes were blotted with antibodies to: CD154 (Santa Cruz Biotechnology cat no. sc978), NFATc1 (Biolegend, cat no.649601), phospho-IKBa (ser32) (Cell Signaling Technology cat no. 9246), IΚBα or ACTIN (Sigma, cat no.A5441).
Brummer G., Fang W., Smart C., Zinda B., Alissa N., Berkland C., Miller D, & Cheng N. (2019). CCR2 signaling in breast carcinoma cells promotes tumor growth and invasion by promoting CCL2 and suppressing CD154 effects on the angiogenic and immune microenvironments. Oncogene, 39(11), 2275-2289.
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