A CCK8 kit (Biosharp, CA, USA) was utilized to detect the cell viability. The transfected cells were planted in 96-well plates with 5000 cells per well. After a period of culture, these cells were incubated with CCK8 reagent for 1 h. Finally, the absorbance (450 nm) was measured and analyzed using a microplate reader (Molecular Devices, CA, USA). For the EdU assay, after incubation with the EdU solution (Beyotime, Shanghai, China) in a dark environment at 37 °C for 1 h, the cells were immobilized with paraformaldehyde for 20 min, and DAPI (Sigma, Shanghai, China) was utilized to visualize the nucleus. Subsequently, EDU-positive cells were captured and counted using an Olympus fluorescent microscope.
Cck 8 kit
Manufactured by Biosharp
Sourced in China
The CCK-8 kit is a colorimetric assay for the determination of cell viability. It utilizes the highly water-soluble tetrazolium salt WST-8, which is reduced by dehydrogenases in living cells to produce an orange-colored formazan dye. The amount of formazan dye generated is directly proportional to the number of living cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Antibody for glyceraldehyde 3 phosphate dehydrogenase gapdh, by Merck Group (2 mentions)
Snail, by Abcam (2 mentions)
Vimentin, by Abcam (2 mentions)
E cadherin, by Abcam (2 mentions)
N cadherin, by Abcam (2 mentions)
Microplate reader, by Agilent Technologies (2 mentions)
Microplate reader, by Allsheng (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Edu solution, by Beyotime (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Fluorescent microscope, by Olympus (1 mentions)
Microplate reader, by Molecular Devices (1 mentions)
Coptisine, by Merck Group (1 mentions)
Mfg e8 antibody, by Santa Cruz Biotechnology (1 mentions)
Neutral gum, by Biosharp (1 mentions)
Annexin 5 fitc pi apoptosis kit, by MultiSciences Biotech (1 mentions)
Primescript rt master mix, by Takara Bio (1 mentions)
54 protocols using cck 8 kit
1
Cell Viability and Proliferation Assay
2
Cell Viability Evaluation via CCK-8 Assay
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A CCK-8 kit (Biosharp, China) was used to evaluate the cell viability, according to the manufacturer’s instructions. The cells were inoculated into 96-well plates. After the cells adhered to the walls of the culture plate, drugs of different concentration gradients were added to each well for 24/48 h, then the CCK-8 reagent was added into 96-well plates (10 µL/well) and incubated at 37 °C for 2 h. The absorbance was measured at 450 nm. Cell viability was calculated by the formula (cell viability (%) = [A (dosing) − A (blank)]/[A (0 dosing) − A (blank)] × 100).
3
Coptisine and MFG-E8 Antibody Assay
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Coptisine was purchased from Sigma-Aldrich (St. Louis, MO, USA). MFG-E8 antibody was purchased from Santa Cruz (USA). Antibodies for PI3K, AKT, p-AKT, MMP-9, MMP-2, E-cadherin, snail, N-cadherin and vimentin were purchased from Abcam (CA, USA). Antibody for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was from Sigma-Aldrich (St. Louis, MO, USA). CCK8 kit was purchased from Biosharp (Hefei, China). All other chemicals were from Sigma-Aldrich.
4
Chitosan-based Antimicrobial Nanoparticles
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CA (Table S1) and CS were purchased from Macklin Biological Co., Ltd. (Shanghai, China), the average molecular weight of CS is 3.0 × 105 Da, and the degree of deacetylation is about 10%. Soy lecithin was purchased from Pengrui Biomedicine Co., Ltd. (Pizhou, China). Hematoxylin and eosin (HE) dye solution, neutral gum, and CCK-8 kit were purchased from BioSharp Co., Ltd. (Hefei, China). Miglyol 812N was purchased from Beijing Fengli Jingqiu Pharmaceutical Co., Ltd. (Beijing, China). Phosphate buffered solution (PBS) solution was prepared with NaCl, KCl, Na2HPO4, and KH2PO4. The pH value of PBS was adjusted with hydrochloric acid and sodium hydroxide to 7.4, and the concentration of PBS is 0.1 M. The model strain of S. mutans (UA159) was donated by the stomatology laboratory of Zunyi Medical University, and human oral epithelial cells (HOECs) were derived from the cell-sharing platform of Zunyi Medical University. Sprague-Dawley rats were purchased from Zhuhai BesTest Bio-Tech Co., Ltd. (Zhuhai, China).
5
SNU1076 Cell Line Culture and Transfection
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SNU1076 cell lines were purchased from the Cell Bank of the Shanghai Institute of Cells, Chinese Academy of Science (Shanghai, China). The cell was cultured in RPMI 1640 medium (Gibco, Billings, MT, USA) containing 10% fetal bovine serum (FBS, BI), 100 U/mL penicillin, and 100 μg/Ml streptomycin at 37 °C in a 5% CO2 environment. RiboPECTTM CP Transfection Kit was obtained from Ribobio (Guangzhou, China). RNA Quick Purification kit was purchased from ESscience. PrimeScript RT Master Mix was purchased from TAKARA. CCK8 Kit was purchased from Biosharp. SiRNA NTMT1 was synthesized from Ribobio (Guangzhou) were synthesized using sequences provided in the relevant literature (Zhang & Song, 2021 (link)). siNTMT1-1: GCAUUGGGAGGAUCACCAATT, siNTMT1-2: GCAAGAGGGTGAGGAAC TA. Anti-γ-H2AX was purchased from CST. Annexin V-FITC/PI apoptosis kit was purchased from MultiSciences.
6
Evaluating HSC-3 Cell Viability with CCK-8
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A CCK-8 kit (Biosharp, China) was used to detect the viability of HSC-3 cells. At 8 h after co-infection with PNB, HSC-3 cells were washed three times with PBS (GIBCO, USA) and then were cultured for another 12 h with metronidazole (200 μg/ml) (Solarbio, China) and gentamicin (300 μg/ml) (Solarbio, China) in DMEM [24 (link)]. Those cells were seeded in 96-well plates at 3 × 103 cells/well and cultured for 24, 48, 72, and 96 h. Ten μL CCK-8 solution was added to the medium for 2 h at room temperature and OD450 values were detected using a Varioskan Flash microplate reader (Thermo Fisher Scientific, USA).
7
Fisetin Cytotoxicity Assay on HPNE, PANC-1, Patu-8988
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The CCK-8 Kit (Biosharp, BS350B) was employed to explored viability of HPNE, PANC-1, as well as Patu-8988 cells inoculated with fisetin (160 μM, 140 μM, 120 μM, 100 μM, 80 μM, 60 μM, 40 μM, 20 μM, and 0 μM) and allowed to grow for 24 hours. After that, we introduced 10 μL of the reagent to the well harboring 100 μL of 5 × 103 cell suspensions incubated for one hour, and OD read at 450 nm. Cell viability percentage was computed via comparison with the control cells (untreated).
8
Cell Viability Assessment with CCK-8 Kit
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A CCK‐8 kit (Biosharp) assay was used to evaluate cell viability and performed as previously described.45
9
Parecoxib-Induced EMT Pathway Study
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ParecoxibNa was purchased from Pfizer (Pfizer Inc., New York, USA). Antibodies for ELMO3, COX-2, E-cadherin, Snail, N-cadherin, and Vimentin were purchased from Abcam (CA, USA). Antibody for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was from Sigma-Aldrich (St. Louis, MO, USA). CCK8 kit was purchased from Biosharp (Hefei, China).
10
Cell Viability Assay of SCC7 Cells
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The CCK-8 kit (Biosharp catalog no. BS350B) was used to measure the cell vitality of SCC7 cells. After washing with PBS, CCK-8 was added after 6, 12, 24, and 48 h. The absorbance value of each well was measured at 450 nm.
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