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Synamps2 system

Manufactured by Compumedics
Sourced in United States, Australia

The SynAmps2 system is a high-performance, multi-channel electroencephalography (EEG) and event-related potential (ERP) amplifier designed for use in research and clinical applications. The system provides precise, low-noise amplification and digitization of electrophysiological signals, enabling accurate measurement and analysis of brain activity.

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28 protocols using synamps2 system

1

Acquisition and Pre-processing of EEG Data

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EEG signals were obtained through the SynAmps2 System (Compumedics, Neuroscan) in an electrically shielded, sound-attenuated room. Recorded EEG signals were collected using a 32-channel Quick-Cap, which had 30 Ag/AgCl electrodes (FP1, FP2, F7, F3, FZ, F4, F8, FT7, FC3, FCZ, FC4, FT8, T7, C3, CZ, C4, T8, TP7, CP3, CZ, CP4, TP8, P7, P3, PZ, P4, P8, O1, O2, and OZ) mounted on it according to the international 10–20 system and two reference electrodes placed at the opposite lateral mastoids. EEG data were sampled at 500 Hz. To assure the collection of high-quality data, impedance for the previously mentioned channels was kept below 5 kΩ. Horizontal and vertical eye movements were monitored with electrooculogram (EOG) electrodes. Horizontal electrooculogram (hEOG) electrodes were placed above and below the left eye, and vertical electrooculogram (vEOG) electrodes were placed near the outer canthi of each eye. Impedance for the EOG channels was kept below 10 kΩ.
The EEG and EOG signals were baseline corrected and bandpass filtered between 0.3 and 30 Hz. Ocular artifacts were corrected by applying the covariance method with the data from both hEOG and vEOG channels. The data for each were then epoched from −200 to 1,400 ms with reference to stimulus onsets. Extracted epochs were grouped and labeled by trial type (i.e., old, new, and control).
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2

Scalp EEG Recording Methodology

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Electrophysiological recordings were acquired using a SynAmps2 system and Scan 4.1 software (Compumedics Neuroscan, Charlotte, NC, USA). Ag/AgCl electrodes hand placed at scalp locations Fz, Cz, Pz, and referenced through the left mastoid (off-line the average of both the right and left mastoids were used as the reference). Forehead served as ground. Measurements were taken in according to the 10–20 international placement guide (Jasper, 1958 ). The recordings were sampled at a rate of 1000 Hz with a 0.1 Hz high-pass and 200 Hz low-pass recording filter. Ocular movements were determined through superior and lateral eye electrodes. Impedances were below 10 kΩ.
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3

Detailed EEG Recording Protocol

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EEG activity was recorded using a 32-channel Quik-Cap and amplified with a SynAmps 2 system (Compumedics Neuroscan; Charlotte, NC, USA). The ground electrode was placed on the mid-frontal scalp, and all channels were referred to the left (M1) side of the mastoid. The montage included 6 midline sites (FZ, FCZ, CZ, CPZ, PZ, and OZ) and 12 sites over each hemisphere (Fp1/Fp2, F3/F4, F7/F8, FC3/FC4, FT7/FT8, C3/C4, T3/T4, CP3/CP4, TP7/TP8, P3/P4, T5/T6, and O1/O2). Additional electrodes were employed to monitor ocular artefacts. Electrodes above and below the left eye were used to record the vertical electrooculogram (EOG). The horizontal EOG was recorded from the outer canthus of each eye. The impedance was maintained at or below 5 K before beginning the EEQ acquisition. At each electrode location, continuous EEG activity was sampled at 1000 Hz, amplified with SynAmps 2 AC amplifiers, and digitised at a 24-bit resolution.
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4

High-Density EEG Recordings in Shielded Room

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Participants performed the task in an electrically shielded room while seated in a comfortable high-back chair. The electrophysiological recordings were done using 128 EEG channels with an electrode placement based on the 10% system (EasyCap Montage No. 15, http://www.easycap.de/). The sampling rate during recording was set to 1000 Hz. The electrodes used were EasyCap active ring electrodes (Ag/AgCl) with impedance conversion circuits integrated into the electrode housing that allows high quality recordings even with high impedance values, thus reducing preparation time and noise. The signals were amplified via a Neuroscan SynAmps2 system and filtered online with a 40 Hz low-pass and a 0.15 Hz high-pass analog filter prior to digitization and saving of the continuous data set. During recording, all electrodes were referenced to an electrode placed on the left mastoid. Eye blinks were recorded with one electrode above and one electrode below the left eye, and a ground electrode was placed anteriorly on the midline.
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5

EEG Acquisition and Processing for BCI

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In this study, the EEG data was recorded by the Neuroscan Synamps2 system with 64 electrodes. One referenced electrode was in the central area near Cz and one ground electrode was on the frontal lobe. The sampling rate of the system was set to 1,000 Hz and a 50 Hz notch filter was applied during the EEG acquisition. In the pre-processing, the EEG was firstly re-referenced to the average value of left and right mastoids M1, M2, and then filtered at 1–15 Hz by Chebyshev II filters. To make the data easy to calculate and store, the filtered EEG was down sampled at 500 Hz. This study used MetaBCI for realizing real-time high-speed data retrieving, data offline/online processing and output feedback. MetaBCI is a one-stop software for the construction of BCIs which was proposed as the first open-source software for BCIs in China.1
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6

EEG Recording in Electromagnetic Shielding

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The experiment was performed in an electromagnetic shielding room. EEG data was recorded with a SynAmps2 system (Neuroscan, EI Paso, TX, USA). An elastic cap with 32 mounted Ag/AgCI electrodes were positioned according to the extended 10–20 system which was used to detect EEG activities with the reference at the vertex. The EEG electrode impedances were kept below 5 kΩ during the entire experiment. All data were referenced against an electrode centered on the midline between Fz and Cz. EEG data were band-pass filtered (0.5–100 Hz). All signals were sampled to disk at 1,000 Hz together with event markers and saved on a hard disk for off-line analysis.
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7

Comparative EEG Analysis of Stroke Patients

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EEG signals of stroke patients were recorded using a BrainAmp amplifier (Brain Products, Gilching, Germany) and 32 channels of active Ag/AgCl electrodes (actiCAP, Brain Products, Germany). The low-pass filter setting was 0–100 Hz with a sampling rate of 200 Hz, and a 50 Hz notch filter was used to diminish power line interference. EEG signals of healthy subjects were recorded using a SynAmps2 system (NeuroScan, U.S.A.) and a quick-cap with 64 Ag/AgCl electrodes. The sampling rate of healthy subjects was 250 Hz and raw data were filtered with an analog bandpass filter from 0.5 to 70 Hz and a notch filter of 50 Hz. The electrodes were placed according to the extended 10–20 system. The ground channel was located on the forehead, and the reference channel was located on the vertex. Impedances of all electrodes were kept below 5 kΩ. Different EEG recording systems were used for healthy subjects and stroke patients, because the experiments of stroke patients were carried out in a clinical setting and only the BrainAmp system was allowed and approved in the ethics. Although 64 channels of EEG signals were recorded from healthy subjects, only 32 channels which had the same locations of electrodes as those of stroke patients were used in further analysis.
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8

EEG Recording and Preprocessing for Cognitive Studies

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Here, the EEG recordings were captured using the Synamps2 system (Neuroscan, Inc., Brentwood, UK) at a sampling frequency of 1000 Hz. The EEG data were stored in the “.cnt” format, incorporating all 64 Ag/AgCl electrode channels arranged in alignment with the 10–20 system. The reference electrode was positioned at the left mastoid, and the impedance of the electrodes was kept under 10 kΩ. The system applied a filter to the EEG data ranging from 0.1 Hz to 100 Hz. Additionally, to eliminate interference from electrical power sources, the EEG data underwent filtering to exclude frequencies above 50 Hz. Two malfunctioning channels (M1 and M2), which exhibited electrode impedances exceeding 10 kΩ, were excluded from the analysis.
During the preprocessing stage, the continuous EEG recordings were subjected to a bandpass filter from 2–30 Hz using a 4th-order Butterworth filter. The data for each block were divided into EEG trials, with each trial being associated with a specific picture. In the experimental design of this study, each participant was presented with 14 blocks of EEG samples, with each block consisting of 100 trials, during a single session.
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9

High-Density EEG Scalp Mapping Protocol

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Participants were seated in a comfortable chair in a sound attenuated, darkened room. An electro-cap fitted with tin electrodes was used to record continuous EEG from 29 sites on the scalp including sites over left and right fronto-polar (FP1/FP2), frontal (F3/F4, F7/F8), frontal-central (FC1/FC2, FC5/FC6), central (C3/C4), temporal (T5/T6, T3/T4), central-parietal (CP1/CP2, CP5/CP6), parietal (P3/P4), and occipital (O1/O2) areas and five midline sites over the frontal pole (FPz), frontal (Fz), central (Cz), parietal (Pz) and occipital (Oz) areas (see Figure 2). Four additional electrodes were attached: one below the left eye (to monitor for vertical eye movement/blinks – LE), one to the right of the right eye (to monitor for horizontal eye movements – HE), one over the left mastoid (reference) and one over the right mastoid (recorded actively to monitor for differential mastoid activity). All EEG electrode impedances were maintained below 2.5 kΩ (impedance for eye electrodes was less than 5 kΩ). The EEG was amplified by a NeuroScan Synamps 2 system with a bandpass of DC to 200 Hz and the EEG were continuously sampled at a rate of 500 Hz.
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10

64-Channel EEG Recording with Neuroscan

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The 64-channel electrode array included the 10-20 montage. Neuroscan Synamps 2 system and its accompanying SCAN 4.3 software system were used to record EEG data.
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