MOLM-13, OCI-AML3, or MV4-11 (156 cells per condition) were plated with DMSO or 1 µM EPZ in MethoCult H4035 Optimum Without EPO (StemCell Technologies, Cambridge, MA, USA) and incubated at 37 °C with 5% CO2 for 7 days. Images were taken with the STEMVision Instrument, and the colonies were counted manually. For primary AML samples, cells were plated at the appropriate density (5 × 104–2 × 105 cells/well) with DMSO or 1 µM EPZ in MethoCult H4035 Optimum Without EPO and incubated at 37 °C with 5% CO2 and 1% O2 for 14 days. Images were taken with the STEMVision Instrument, and colonies were counted using the appropriate STEMVision algorithm or manually by two counters blinded to experimental conditions on the Revolve Microscope (ECHO). Samples that produced fewer than 20 colonies counted in the DMSO condition were excluded. For the normal donor CD34+ CFU assays, normal bone marrow was obtained from AllCells. The Human CD34 MicroBead Kit (Miltenyi Biotec, Gaithersburg, MD, USA) was used to isolate CD34+ cells. A total of 1000 CD34+ cells/well were plated with DMSO or 1 µM EPZ in MethoCult H4035 Optimum Without EPO and incubated at 37 °C with 5% CO2 and 1% O2 for 7–10 days. Colonies were counted by two counters blinded to the experimental conditions using the Revolve Microscope (ECHO). Values are reported as averages of the two separate counters.
Revolve microscope
Manufactured by Echo Medical Systems
Sourced in United States
The Revolve microscope is a high-performance laboratory instrument designed for detailed analysis and observation. It features advanced optics and a stable mechanical design to provide clear, detailed images of samples.
Automatically generated - may contain errors
Lab products found in correlation
Nunc glass base dish, by Thermo Fisher Scientific (1 mentions)
Apoptosis and necrosis quantification kit, by Biotium (1 mentions)
Lsrii sorp, by BD (1 mentions)
Sh30028ls, by Corning (1 mentions)
Bdb550825, by BD (1 mentions)
Mitoview green, by Biotium (1 mentions)
Human cd34 microbead kit, by Miltenyi Biotec (1 mentions)
Methocult h4035 optimum without epo, by STEMCELL (1 mentions)
Neutral buffered formalin, by Merck Group (1 mentions)
Mouse on mouse m o m basic kit, by Vector Laboratories (1 mentions)
Ethanol, by Merck Group (1 mentions)
Vectastain abc kit, by Vector Laboratories (1 mentions)
Echo pro, by Echo Medical Systems (1 mentions)
Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions)
Bz x710, by Keyence (1 mentions)
12 protocols using revolve microscope
1
In Vitro Colony Forming Assay
2
Culturing Human Cerebral Organoids with Xanthan Gum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At day 6 of differentiation, five hCO were transferred into one well of 24 well plate containing culture media with 0.1% (wt/vol) of polymers (see Supplementary Table 1). At day 25, the remaining organoids in each well were imaged using REVOLVE microscope (ECHO), and their number were counted. For comparing to hCO cultured in STEMdiff TM Neural Organoid Basal Medium 2 (hCO + STEMdiff TM NOBM, STEMCELL Technologies, 08620), the remaining organoids in each well were imaged at day 12 on a REVOLVE microscope (ECHO) or a BZX-710 (KEYENCE) with 4x lens. To prepare the XG-supplemented media, the XG powder, which is difficult to dissolve, was mixed with 100% ethanol. More specifically, to make 250 ml of XG-supplemented Neurobasal medium (0.2% XG [wt/vol], which is a 2x stock solution), 0.5 g of XG powder was mixed with 2 ml of 100% ethanol, and 248 ml of Neurobasal was then immediately added on top and mixed to avoid the formation of XG clumps. To avoid contamination, dedicated equipment was used inside a biosafety cabinet to weight XG and to mix the medium.
3
Cellular Uptake Kinetics of Nanoparticles
4
Plaque Assay for Rickettsiae Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Vero cells were seeded in a 24-well plate. A series of 10-fold dilutions of rickettsiae, in 200 μL of DMEM containing 10% FBS, were added to each well containing a confluent monolayer of Vero cells. After cells were incubated with rickettsiae at RT for 6 h, they were placed at 37 °C with 5% CO2 overnight before a semi-solid agarose gel overlay was applied on top of the infected Vero cells. After the gel was solidified at RT, the plate was incubated at 37 °C with 5% CO2. Plates were monitored daily by the Revolve microscope (Echo Inc., San Diego, CA, USA). After 3–5 days, plaques were imaged and measured with an Apple iPad digital camera attached to the microscope and further analyzed using Echo Revolve software.
5
Cell Cycle Analysis and Apoptosis Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two million cells were fixed with 95% precooled ethanol, incubated overnight at −20 °C, washed with Dulbecco’s Phosphate Buffered Saline (DPBS, SH30028LS, Corning), and stained with propidium iodide/RNase staining buffer (BDB550825, BD Biosciences) containing 0.1% sodium citrate for 45 min (min) on ice in the dark. These cells were then analyzed by flow cytometry using BD LSRII SORP (BD Biosciences). ModFit LT software (Verify Software House) was used to generate DNA histograms. Each experiment was performed in triplicates.
The Apoptosis and Necrosis Quantification Kit (30017, Biotium) was used to quantify apoptotic and necrotic TNBC cells at 5 days post-transduction, according to the manufacture’s protocol. Images were taken using a Revolve microscope (ECHO) and data were analyzed by Image J software. Apoptotic and necrotic cells were counted and normalized relative to the total number of cells in each well.
The Apoptosis and Necrosis Quantification Kit (30017, Biotium) was used to quantify apoptotic and necrotic TNBC cells at 5 days post-transduction, according to the manufacture’s protocol. Images were taken using a Revolve microscope (ECHO) and data were analyzed by Image J software. Apoptotic and necrotic cells were counted and normalized relative to the total number of cells in each well.
6
Imaging ACT-Treated CAG12184 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CAG12184 cells grown in SILAC medium were treated with 4 μg/mL ACT, collected at indicated time points, washed, and resuspended with PBS to an OD of 1. 2 μL of the cell suspension was spotted onto an agarose (1%)-coated glass slide, and covered with a glass coverslip. Bright field microscopy images were obtained with Revolve Microscope (Echo) with a 100x oil immersion objective.
7
Mitochondria Staining in Fixed Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mitochondria staining of fixed neurons was performed using MitoView™ Green (Biotium, 70054) as the manufacturer’s instruction. Briefly, fixed symNs were incubated with 100 nM dye solution in PBS at RT for 15 min. For co-staining with regular immunofluorescent stain, neurons were stained with MitoView afterward. After staining neurons were imaged using the ECHO Revolve Microscope.
8
Immunohistochemistry of Mouse Tumor Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Extracted tumors from mice were fixed with 10% neutral buffered formalin (Sigma) for 24 h and changed to 70% ethanol (Sigma). Tumors were paraffin-embedded and sectioned by the Brain Tumor Research Center at UCSF and CHLA. For IHC, slides were deparaffinized, and antigen retrieval was performed using a pressure cooker. The Mouse on Mouse (M.O.M.) basic kit (Vector laboratories) was used for masking endogenous mouse antigen. The VECTASTAIN ABC kit (Vector laboratories) was used for signal detection. Images were taken using the Revolve microscope (ECHO).
9
Inducible GFP-CFTR Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The expression of recombinant GFP-P2A-CFTR proteins in 16HBEge-GFP-P2A-WT/W1282X/Δex23 cells was induced with growth medium containing 0 to 2 μg/mL dox for 48 h (Research Products International Corp, D43020-100). The images were taken with a Revolve microscope and acquired by ECHO Pro version 6.0.1 software (Revolve, ECHO). GFP-intensity analysis from the images was performed with ImageJ2 software (version 2.2.0).
10
Immunohistochemical Analysis of EMT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PDX72 tumor tissues were fixed with 3.7% formaldehyde for 48 h and embedded with paraffin. Tissue blocks were cut into 5 μm slides. IHC was performed as previously described 26 (link) to examine expression of E-cadherin and Vimentin using the corresponding antibodies (Table S2 ). Slides were imaged under Revolve microscope (ECHO).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!