Water purification system

Manufactured by Sartorius

Sourced in Germany, France

The Water Purification System is a laboratory equipment designed to produce high-quality purified water. It utilizes a multi-stage filtration process to remove contaminants and impurities from the water, ensuring consistent and reliable purity levels.

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5 protocols using water purification system

Development and Validation of TD-GC-qMS Method

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The TD-GC-qMS method development was done using dimethyl sulphide (1, anhydrous, ≥ 99%), 2-methylbutanal (2, 95%), dimethyl disulphide (3, ≥ 98%), 2-hexanone (4, analytical standard), 1-octanol (6, anhydrous, ≥ 99%), 2-nonanone (7, ≥ 99%), 1-undecene (8, 97%), 1-decanol (9, ≥ 9 8%) and 2-aminoacetophenone (10, analytical standard) purchased from Sigma-Aldrich (Taufkirchen, Germany), and 2-heptanone (5, ≥ 98%) purchased from Merck KGaA (Darmstadt, Germany).
To verify the analysis of cyclic siloxanes by atmospheric pressure ionization (APPI), coupled with thermogravimetry (TG) and a qMS, three cyclic siloxanes were analysed as single standards. Octamethylcyclotetrasiloxane (D4, 98%, Alfa Aesar, Karlsruhe, Germany), decamethylcyclopentasiloxane (D5, 97%, Alfa Aesar, Karlsruhe, Germany) and dodecamethylcyclohexasiloxane (D6, 95%, Alfa Aesar, Karlsruhe, Germany) were used.
For all purposes, liquid chromatography-mass spectrometry (LC-MS) grade methanol from VWR (Leuven, Belgium) and ultrapure water, generated with a water purification system from Sartorius Stedim (Göttingen, Germany), were used. For cleaning purpose, Decon 90 from VWR (Leuven, Belgium) was used.

Koehler T., Ackermann I., Brecht D., Uteschil F., Wingender J., Telgheder U, & Schmitz O.J. (2020). Analysis of volatile metabolites from in vitro biofilms of Pseudomonas aeruginosa with thin-film microextraction by thermal desorption gas chromatography-mass spectrometry. Analytical and Bioanalytical Chemistry, 412(12), 2881-2892.

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Synthesis of ZnO-based Optoelectronic Materials

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Triethoxysilane (TES) was purchased from Tokyo Chemical Industry Co., LTD. (Tokyo, Japan). Colloidal ink of zinc oxide (ZnO), 1-decene, hydrochloric acid (HCl) and molybdenum (IV) oxide (MoO₃, 99.97% trace metal basis) were purchased from Sigma-Aldrich and used as received. 4,4′-Bis(carbazole-9-yl)biphenyl (CBP, 99.9% trace metals basis) was purchased from Luminescence Technology Corp. Electronic-grade hydrofluoric acid (HF, 48% aqueous solution) was purchased from Kanto Chemical Co., INC. Toluene (HPLC-grade), chloroform, ethanol (99.5), methanol, and Zn powder were purchased from Wako Chemical. Milli-Q water (resistivity = 18.2 MΩ·cm) was obtained by Sartorius water purification system (arium 611 UV).

Yamada H., Watanabe J., Nemoto K., Sun H.T, & Shirahata N. (2022). Postproduction Approach to Enhance the External Quantum Efficiency for Red Light-Emitting Diodes Based on Silicon Nanocrystals. Nanomaterials, 12(23), 4314.

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Quantitative Analysis of Mildronate

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1,1‐Dimethylhydrazine, tert‐butylhydroquinone, methyl‐acrylate, dimethylsulphate‐D₆, calcium hydroxide and the reference compound of Mildronate dihydrate were obtained from Sigma‐Aldrich (Deisendorf, Germany), while Mildronate‐D₃ was synthesized in house for the use as internal standard (IS). Acetonitrile and acetone were bought from VWR International (Darmstadt, Germany). Acetic acid, ammonium acetate, and ethanol were supplied by Merck (Darmstadt, Germany). Deionized water was obtained from a water purification system (Sartorius Stedim Biotech S.A., Aubagne, France).

Görgens C., Guddat S., Dib J., Geyer H., Schänzer W, & Thevis M. (2015). Mildronate (Meldonium) in professional sports – monitoring doping control urine samples using hydrophilic interaction liquid chromatography – high resolution/high accuracy mass spectrometry. Drug Testing and Analysis, 7(11-12), 973-979.

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Electrochemical Characterization of Modified Electrodes

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A three-electrode cell was used for performing electrochemical experiments, with a glassy carbon electrode (GCE) or modified GCE, a platinum wire (d = 0.5 mm), and an Ag|AgCl|KClsat electrode employed as the working, counter, and reference electrodes, respectively. Cyclic voltammetry measurements were performed using a µAutolabIII (Metrohm Autolab) potentiostat powered by GPES 4.9 software. All experiments were performed at ambient temperature (22 ± 3 °C). XPS analysis was performed with a Scanning XPS Microprobe instrument -PHI 5000 VersaProbe (ULVAC-PHI, Japan/USA); details are described elsewhere [18] (link). TEM images were obtained and analyzed with a transmission electron microscope (Tecnai G2 Spirit BioTWIN). Demineralized and ultrapure water, with a specific resistivity of 18.2 MΩ cm, from a water purification system (Sartorius), was utilized for experiments.

Szot-Karpińska K., Kudła P., Szarota A., Narajczyk M., Marken F, & Niedziółka-Jönsson J. (2020). CRP-binding bacteriophage as a new element of layer-by-layer assembly carbon nanofiber modified electrodes. Bioelectrochemistry (Amsterdam, Netherlands), 136.

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Bovine Colostrum Immunoglobulin Purification

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Bovine colostrum powder was purchased from Kanpure Corporation (Heilongjiang, China). Bovine colostrums were obtained from Xiaoshan dairy farm (Hangzhou, China). The mixed-mode resin used in this study was HCIC resin; MEP HyperCel was purchased from Pall Corporation. Bovine IgG standard sample was purchased from Sigma (St. Louis, MO). Hi-Trap Protein G column was purchased from GE Healthcare (Piscataway, NJ). Electrophoresis kits were purchased from Bio-Rad (Hercules, CA). Deionized water used for buffer solutions was prepared by water purification system from Sartorius (Gottingen, Germany). All chemical reagents were analytical grade. The ÄKTA explorer 100 systems were purchased from GE Healthcare (Uppsala, Sweden).

Wu M., Zhang F., Liang Y., Wang R., Chen Z., Lin J, & Yang L. (2015). Isolation and purification of immunoglobulin G from bovine colostrums by hydrophobic charge-induction chromatography. Journal of dairy science, 98(5).

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