An enhanced mitochondrial membrane potential assay kit with JC-1 (Beyotime) was used to examine BMSCs treated with H2O2 and cisplatin. A Reactive Oxygen Species (ROS) Assay Kit (Beyotime) was used to detect the antioxidative capacity of cells. Both JC-1 and ROS assays were observed under a fluorescence microscope.
Assay kit with jc 1
Manufactured by Beyotime
Sourced in China
The Assay kit with JC-1 is a laboratory product designed for scientific research purposes. The kit utilizes the fluorescent dye JC-1 to measure mitochondrial membrane potential, which is an indicator of cellular health and function. The core function of this product is to provide researchers with a tool to assess mitochondrial activity in various experimental models.
Automatically generated - may contain errors
4 protocols using assay kit with jc 1
1
Mitochondrial Potential and ROS Assays
2
Quantifying Mitochondrial Membrane Potential
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ΔΨm was determined by an assay kit with JC-1, according to the manufacturer's instructions (Beyotime Institute of Biotechnology, Haimen, Jiangsu. China). JC-1 stains the mitochondria in cells with a high ΔΨm by forming red fluorescence J-aggregates, whereas in cells with depolarized mitochondria, JC-1 is present as green fluorescent monomers. In this way, mitochondrial depolarization was determined by a decreased ratio of red-to green fluorescence intensity by a fluorescence microscope (Zeiss Inc., Jena, Germany).
3
Assessing Mitochondrial Membrane Potential in GCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Briefly, to examine the MMP of GCs in all treated groups, the mitochondrial membrane potential assay kit with JC-1 (Beyotime, China) was used according to the manufacturer’s instructions. Enzymatic digestion using trypsin was used to harvest the GCs and washed with warm PBS. After collection, the MMP assay kit with JC-1 (Beyotime, China) was used to stain GCs. Flow cytometry was used to count labeled cells using a fluorescence activating cell sorter (FACS) and a Calibur flow cytometer (BD Biosciences, CA, USA). The data was analyzed using FlowJo software (version Win 64-10.4.0).
4
Mitochondrial Membrane Potential Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ΔΨm was determined by an assay kit with JC-1, according to the manufacturer's instructions (Beyotime Institute of Biotechnology). JC-1 stains the mitochondria in cells with a high ΔΨm by forming red fluorescence J-aggregates, whereas in cells with depolarized mitochondria, JC-1 is present as green fluorescent monomers.31 (link) In this way, mitochondrial depolarization was determined by a decreased ratio of red-to-green fluorescence intensity by a fluorescence microscope (Zeiss Inc.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!