Individuals for scanning electron microscopy were sexed and the heads, carrying the antennae, were removed under a stereomicroscope, stored in 70% ethanol and subsequently dehydrated in a graded alcohol series of 80%, 90%, and 100% ethanol. Then, the antennae were mounted on a stub with double-sided adhesive tape and gold sputtered in a Baltec SCD 005 sputter coater. The antennae were mounted on dorsal and ventral sides (both male and female usually keep the antennae extended in front of the head so that the general downward facing curvature of the antennae is retained and a dorsal and ventral side can be easily distinguished) on the stubs and examined and micrographed with a Hitachi TM3030 scanning electron microscope.
Tm3030 scanning electron microscope
Manufactured by Hitachi
Sourced in Japan
The Hitachi TM3030 is a compact scanning electron microscope that provides high-quality imaging and analysis capabilities. It features a simple and intuitive interface, enabling users to easily capture detailed images and perform basic analysis of a wide range of samples.
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Lab products found in correlation
6 protocols using tm3030 scanning electron microscope
1
Antennae Morphology Analysis via SEM
2
Scanning Electron Microscopy of Floral Tissues
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The petals of the ‘FT’, pdm, pdm + JA and pdm + MeJA plants were fixed in a solution of FAA (100 mL: 89 mL 50% ethanol, 6 mL glacial acetic acid, 5 mL formaldehyde) at 4 ℃ for 12 h. The fixed samples were then dehydrated using gradient series of 50%, 75%, 90%, and 100% ethanol (each for 3 min) and then with 50% ethanol + 50% tert-butanol, 25% ethanol + 75% tert-butanol, 10% ethanol + 90% tert-butanol, and 100% tert-butanol (each for 3 min). Thereafter, the samples were immersed in 100% tert-butanol and desiccated in an ES-2030 lyophilizer (Hitachi, Tokyo, Japan). The dried samples were adhered to a sample platform using conductive adhesive and were gold-palladium sputter-coated using an MSP-2S Carbon coater (Hitachi, Tokyo, Japan). Finally, the samples were observed and photographed using a TM3030 scanning electron microscope (Hitachi, Tokyo, Japan).
3
Ultrastructural Examination of Tibial Nerves
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Tibial nerves around ligation or severance sites were removed from mice after fixation with 4% (w/v) paraformaldehyde in PB, then post-fixed with mixed solution, containing 4% paraformaldehyde and 0.5% glutaraldehyde in PB for 20 min. After exposure to 1% (w/v) OsO4 in PB for 2 h at 4°C, specimens were stained with 2% (w/v) uranyl acetate aqueous solution overnight, and embedded in epoxy resin in the usual manner. Semi-thin transverse sections with a 250 nm thickness were stained with lead citrate solution (Reynolds, 1963) and observed under an TM3030 scanning electron microscope using backscattered electron mode (Hitachi, Japan). Image data shown were processed by reversing black/white.
4
SEM Analysis of Spray-Dried Powder
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For scanning electron microscopy (SEM), the spray-dried powder was placed onto sticky carbon tape mounted on SEM stubs and observation was conducted using a field emission TM3030 scanning electron microscope (Hitachi, Japan) at different magnifications (i.e., × 1000 and × 4000).
5
Biochar Characterization Techniques
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The C, H, N, O, and S element contents of the biochars were determined using an element analyzer (Vario MicroCube, Elementar Company, Frankfurt, Germany). The surface functional groups of biochar were determined by Fourier transform infrared spectroscopy (is50 FT-IR, Thermo Fisher Scientific, Waltham, MA, USA) and an X-ray photoelectron spectrometer (Escalab 250Xi, Thermo Fisher Scientific, MA, USA). The Se content was measured by an atomic fluorescence spectrophotometer (AFS-620, Beijing Rayleigh Analytical Instrument Corp, Beijing, China). The Zeta potential was measured using a ZETASIZER 3000 HSA system (Malvern Panalytical, Shanghai, China). The sample was scanned using a TM3030 scanning electron microscope (SEM) (Hitachi, Tokyo, Japan).
6
Scanning Electron Microscopy of Dermanyssus gallinae
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Dermanyssus gallinae specimens used in the current study were sourced from a farm located in the Dutch province of Gelderland. Mites were collected from under the horizontal fixed u-profiles of the aviary housing system using a fine brush, and stored for transport to the laboratory at Wageningen in a vial with a lid. In the laboratory, the collected mites were placed into vials containing 70% ethanol. The mites were then sent to the laboratory at Foggia University, where they were prepared for observation by scanning electron microscopy (SEM).
When at Foggia, mites were dehydrated through a graded ethanol series (80%, 90%, 100%), dried using a Baltec CPD030 critical point dryer, mounted on SEM stubs using conductive carbon adhesive tabs and sputter-coated with palladium-gold using a Baltec SCD005 coating apparatus. Specimens were then examined and micrographed with a Hitachi TM3030 scanning electron microscope, equipped with a digital camera.
When at Foggia, mites were dehydrated through a graded ethanol series (80%, 90%, 100%), dried using a Baltec CPD030 critical point dryer, mounted on SEM stubs using conductive carbon adhesive tabs and sputter-coated with palladium-gold using a Baltec SCD005 coating apparatus. Specimens were then examined and micrographed with a Hitachi TM3030 scanning electron microscope, equipped with a digital camera.
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