Pharmaceutical grade DMSO (99.9%) was obtained from Cayman® Chemicals Company, Ann Arbor, United States. Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), and lipid hydroperoxide (LPO) assay kits were obtained from Cayman® chemical company, Ann Arbor, United States S100B ELISA kit was obtained from Fine Biotech, China. Ketamine hydrochloride was obtained from Rotexmedica®, Trittau, Germany. RIPA Lysis buffer kit was bought from Invitrogen, United States. Primers were sourced from Apical Scientific, Selangor, Malaysia, cDNA synthesis kit, and qPCR kit were obtained from Bioline reagent limited, United Kingdom.
Superoxide dismutase sod
Manufactured by Cayman Chemical
Sourced in United States
Superoxide dismutase (SOD) is an enzyme that catalyzes the dismutation of superoxide radicals into oxygen and hydrogen peroxide. It is an important antioxidant defense in nearly all cells exposed to oxygen.
Automatically generated - may contain errors
Lab products found in correlation
Quantifast sybr green kit, by Qiagen (1 mentions)
Creatinine, by Merck Group (1 mentions)
Catalase activity assay kit, by Cayman Chemical (1 mentions)
Albumin assay kit, by Merck Group (1 mentions)
Elisa kits for il 6 and tnfα, by BD (1 mentions)
Insulin, by Crystal Chem (1 mentions)
8 isoprostane, by Cayman Chemical (1 mentions)
Malondialdehyde mda, by Merck Group (1 mentions)
Troponin 1, by Life Diagnostics (1 mentions)
Catalase, by Cayman Chemical (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
P pten, by Cell Signaling Technology (1 mentions)
Smad2 3, by Cell Signaling Technology (1 mentions)
Smad7, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
4 protocols using superoxide dismutase sod
1
Oxidative Stress Biomarker Assays
2
Coagonist Peptide Synthesis and Analysis
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The coagonist of GCGR and GLP-1R, Aib2 C24 Chimera2 (H1SQGT5FTSDY10SKYLD15EQAAK20EFIAW25LMNT-NH2) was synthesized at Zydus Research Centre, Ahmedabad, India[20 (link)]. Kits for triglycerides, cholesterol, and glucose were purchased from Avantor Performance Materials India Ltd, India. ELISA kits for IL-6 and TNF-α were obtained from BD Biosciences, United States; Insulin from Crystal Chem, United States; FGF21 from Wuhan Eiaab science, Co., Ltd. China; leptin and Adiponectin were purchased from B-bridge international, United States. Superoxide dismutase (SOD) and catalase activity assay kit were obtained from Cayman Chemical, United States. Creatinine, blood urea nitrogen, and albumin assay kit were obtained from Sigma-Aldrich, United States. TRIzol reagent and cDNA reverse transcription kit purchased from Invitrogen, Life Technology, United States and QIAGEN Quanti Fast SYBR Green kit were purchased from Qiagen, Germantown, United States. All other chemicals and reagents were purchased from Sigma-Aldrich chemicals, United States unless stated otherwise.
3
Comprehensive Cardiac Biomarker Assessment
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Preparation of heart homogenates for the assessment of markers of injury inflammation and oxidative stress was achieved as previously reported [19 (link)]. The levels of troponin I (Life Diagnostics, West Chester, PA, USA), tumor necrosis factor α (TNF-α; R&D Systems, Minneapolis, MN, USA), interleukin-1β (IL-1β; R&D systems, Minneapolis, MN, USA), 8-isoprostane (Cayman Chemicals, Michigan, USA), malondialdehyde (MDA; Sigma-Aldrich Fine Chemicals, St. Louis, MO, USA), superoxide dismutase (SOD; Cayman Chemicals, Michigan, USA), and catalase (Cayman Chemicals, Michigan, USA) were assayed according to the protocols described by the respective manufacturers.
4
Hepatoprotective Effects of EX-527
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EX-527 (6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1carboxamide) was procured from Sigma–Aldrich Biotechnology (St. Louis, MO, USA). ELISA kits for the analysis of alanine aminotransferase (ALT), aspartate aminotransferase (AST), r-glutamyl transferase (r-GPT), and alkaline phosphatase (ALP) were procured from Abcam (Cambridge, UK). Assay kits for malondialdehyde (MDA), glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) were purchased from Cayman Chemical (Ann Arbor, MI, USA). Primary antibodies specific to liver fibrosis markers α-SMA, collagen 1, vimentin, Smad2/3, p-Smad2/3, Smad4, Smad7, p-PI3K, PI3K, p-Akt, Akt, p-PTEN, PTEN, Bcl-2, Bax, cleaved-caspase-3, p53, and β-actin were procured from Cell Signaling Technology (Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
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