The human pancreatic cancer cell lines SW1990 and BxPC-3 were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The cells were cultured in Dulbecco's modified Eagle's medium containing 10% foetal bovine serum (Thermo Fisher Scientific) and 1% penicillin-streptomycin at 37 °C with 5% CO2. Adenosine (018–10,492) was purchased from Wako (Osaka, Japan) and 8-CPT (#C0735), DMPX (#D134), alloxan (#A7413), MRS1523 (#M1809), EHNA (#E114), forskolin (#F6886), SQ22536 (#S153), H89 (#B1427), and dipyridamole (#D9766) were purchased from Sigma (Shanghai, China); HPBCD (#A600388) was from Sangon Biotech (Shanghai, China); and GSK690693 (#HY-10249) was from MCE (New Jersey, USA). For in vivo studies, Adenosine and GSK690693 were dissolved in 10% 2-hydroxypropyl-β-cyclodextrin (Sangon Biotech, Shanghai, China).
2 hydroxypropyl β cyclodextrin
Manufactured by Sangon
Sourced in China
2-hydroxypropyl-β-cyclodextrin is a modified cyclodextrin compound. Cyclodextrins are cyclic oligosaccharides that can form inclusion complexes with various guest molecules. 2-hydroxypropyl-β-cyclodextrin is used to enhance the solubility, stability, and bioavailability of certain compounds in pharmaceutical, food, and chemical applications.
Automatically generated - may contain errors
Lab products found in correlation
Dipyridamole, by Merck Group (1 mentions)
Adenosine, by Fujifilm (1 mentions)
Adenosine, by Sangon (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Alloxan, by Merck Group (1 mentions)
Sq22536, by Merck Group (1 mentions)
Forskolin, by Merck Group (1 mentions)
Mrs1523, by Merck Group (1 mentions)
Bxpc 3, by National Collection of Authenticated Cell Cultures (1 mentions)
Sw1990, by National Collection of Authenticated Cell Cultures (1 mentions)
3 protocols using 2 hydroxypropyl β cyclodextrin
1
Pancreatic Cancer Cell Culture Protocol
2
Celastrol Purification and Dissolution
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Celastrol was acquired from MCE (MedChem Express, Shanghai, China), and the purity of the compounds was ≥99.65%. Celastrol was dissolved in dimethyl sulfoxide (DMSO), and DMSO was used as the vehicle control. For in vivo study, celastrol was dissolved first in DMSO and then in 10% 2-hydroxypropyl-β-cyclodextrin (Sangon Biotech, Shanghai, China).
3
Fangchinoline Compound Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fangchinoline was purchased from MCE (MedChem Express, Shanghai, China), and the purity of the compounds was ≥99.92%. FCL was dissolved in dimethyl sulfoxide (DMSO) and stored at −80°C for the in vitro study. For the in vivo study, FCL was dissolved first in 5% DMSO and then in 10% 2-hydroxypropyl-β-cyclodextrin (Sangon Biotech, Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!