Acy1215

Manufactured by Chemietek

The ACY1215 is a laboratory-grade analytical instrument designed for chemical analysis. It features high precision and accuracy for the measurement of various parameters. The core function of this equipment is to provide reliable and consistent data for scientific research and testing purposes.

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Lab products found in correlation

Tubacin, by Merck Group (3 mentions) Mg132, by Merck Group (1 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions) Acy 1215, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Peg400, by Merck Group (1 mentions) Hdac6, by Cell Signaling Technology (1 mentions) Mc1568, by Merck Group (1 mentions) α tubulin, by Cell Signaling Technology (1 mentions) Tubastatin, by Merck Group (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Recombinant human tgf β1, by R&D Systems (1 mentions) Trichostatin a, by Merck Group (1 mentions)

4 protocols using acy1215

Modulation of HDAC6 and Notch1 in Cells

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Tubacin and the proteasome inhibitor, MG132, were purchased from Sigma (St. Louis, MO, USA). ACY1215 was purchased from Chemietek (Indianapolis, IN). siRNA targeting human HDAC6 (SI02663808 [siHDAC6_A], SI02757769 [siHDAC6_B], SI03058706 [siHDAC6_C], and SI04438490 [siHDAC6_D]), Notch1 (SI00119035), and AllStars Negative Control siRNA (SI03650318) was purchased from Qiagen (Valencia, CA, USA). Transfections were conducted using the Lipofectamine 2000 Transfection Reagent following the manufacturer's protocol (Invitrogen).

Deskin B., Yin Q., Zhuang Y., Saito S., Shan B, & Lasky J.A. (2019). Inhibition of HDAC6 Attenuates Tumor Growth of Non-Small Cell Lung Cancer. Translational Oncology, 13(2), 135-145.

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Enhancing ACY-1215 Bioavailability

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The reagent 2-(diphenylamino)-N-(7-(hydroxyamino)-7-oxoheptyl) pyrimidine-5-carboxamide (ACY-1215) was purchased from Chemietek (Indianapolis, IN). ACY-1215 (ACY) was first dissolved in DMSO (Sigma–Aldrich, St. Louis, MO) and then in culture medium (1–2 µM) for cell culture. Because PEG-400 enhances the bioavailability of ACY, it was dissolved in PEG-400 (Sigma–Aldrich) and diluted with sterile water. ACY was administrated to mice by gavage.

Tsuji G., Okiyama N., Villarroel V.A, & Katz S.I. (2014). Histone deacetylase 6 inhibition impairs effector CD8 T-cell functions during skin inflammation. The Journal of allergy and clinical immunology, 135(5), 1228-1239.

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Comprehensive Fibrosis Evaluation Protocol

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Tubacin, Tubastatin, and MC1568 were purchased from Sigma-Aldrich (St.Louis, MO). ACY1215 was purchased from ChemieTek　(Indianapolis, IN). Human recombinant TGF-β1 was purchased from R&D systems (Minneapolis, MN). Bleomycin was purchased from TEVA Pharmaceutical Industries (Petach Tikva, Israel). The following primary antibodies were purchased from the following companies: Santa Cruz (Dallas, TX; HDAC6 [H-300], HIF-1α), Abcam (Cambridge, MA; type-1 collagen), Sigma-Aldrich (α-SMA, acetylated α-tubulin), Cell Signaling Technology (Danvers, MA; β-actin, Smad2, phosphorylated Smad2, Smad3, phosphorylated Smad3, Akt, phosphorylated Akt (Ser473), PHLPP, Erk, phosphorylated Erk, p38, phosphorylated p38, HDAC6 [for the mouse samples], α-tubulin, LC3B). Secondary antibodies used were anti-mouse or anti-rabbit IgG HRP-linked antibodies (Cell Signaling Technology).

Saito S., Zhuang Y., Shan B., Danchuk S., Luo F., Korfei M., Guenther A, & Lasky J.A. (2017). Tubastatin ameliorates pulmonary fibrosis by targeting the TGFβ-PI3K-Akt pathway. PLoS ONE, 12(10), e0186615.

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Histone Deacetylase Inhibitor Effects on Xenograft Tumor Growth

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Cells were treated with 20μM Tubacin (Sigma), 10-20μM Tubastatin A (ChemScene), and 2μM ACY1215 (ChemieTek) or 1μg/ml Trichostatin A (Sigma) for 2-12h, and disrupted in a PTY buffer (35 (link)) and processed for WB or IP. b) Xenograft studies. Compound administration began 1 week after the injection of the tumor cells into the mammary fat pad of the mice. Tubastatin A was dissolved to 70mg/ml in DMSO and brought to 10mg/ml with PBS. A 40 mg/kg/dose was administered via intra peritoneal injection once a day for 5 days/week for a total of 2 weeks (36 (link)). For the lung metastasis studies, Tubastatin A was administered in the same manner as above, starting 24 h after the tumor cell tail vein injections. ACY-1215 was dissolved to 40mg/ml in DMSO and brought to 24mg/ml with saline. A 75 mg/kg/dose was administered via intra peritoneal injection once a day for 5 days/week for a total of 2 weeks (37 (link)).

Kozyreva V.K., McLaughlin S.L., Livengood R.H., Calkins R.A., Kelley L.C., Rajulapati A., Ice R.J., Smolkin M.B., Weed S.A, & Pugacheva E.N. (2014). “NEDD9 Regulates Actin Dynamics through Cortactin Deacetylation in an AURKA/HDAC6-dependent Manner”. Molecular cancer research : MCR, 12(5), 681-693.

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