Fl 1201

Manufactured by Vector Laboratories

Sourced in United States

The FL-1201 is a spectrofluorometer designed for the detection and analysis of fluorescent samples. It features a wide excitation and emission wavelength range, allowing for the study of a variety of fluorescent molecules. The FL-1201 provides accurate and reliable fluorescence measurements, making it a versatile tool for various scientific applications.

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Lab products found in correlation

7 protocols using fl 1201

Suprachoroidal Nanoparticle Delivery and Imaging

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At several time points after suprachoroidal injection of 3 μl of NPs containing 1 μg of pEGFP-N1, rats were euthanized and eyes were frozen in optimal cutting temperature embedding compound (Miles Diagnostics, Elkhart, IN). Frozen sections (10 μm) were fixed in 4% paraformaldehyde, and nonspecific binding was blocked by a 30-min incubation in 8% normal rabbit serum at 25°C. The sections were incubated with a polyclonal antibody (1:300) against EGFP conjugated with Alexa Fluor 594 (A-21312, ThermoFisher, Waltham, MA) at 23°C for 2 hours. After washing with PBS containing 0.05% Tween 20, slides were counterstained with Hoechst 33258 (861405, Sigma-Aldrich, St. Louis, MO) and viewed with a Nikon fluorescence microscope. Ocular sections from rats that had been given a suprachoroidal injection of NPs containing VEGF expression plasmid or RPE whole mounts from rho/VEGF mice were stained with fluorescein isothiocyanate–labeled (FL-1201, Vector Laboratories, Burlingame, CA) or DyLight 594–labeled Griffonia simplicifolia lectin (DL-1207, Vector Laboratories), which selectively stains vascular cells as previously described (38 (link)).

Shen J., Kim J., Tzeng S.Y., Ding K., Hafiz Z., Long D., Wang J., Green J.J, & Campochiaro P.A. (2020). Suprachoroidal gene transfer with nonviral nanoparticles. Science Advances, 6(27), eaba1606.

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Histological Analysis of Cardiac Tissue

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At day
28, after the completion of the study, animals were sacrificed and
the hearts resected. The hearts were fixed in 10% formalin overnight
and then transferred to 30% sucrose buffer for 2–3 days until
the sucrose penetrated through the tissue (and the hearts “sank”).
The hearts were then embedded in an optimal cutting temperature (OCT)
compound (Tissue-Tek, Fisher Scientific) and stored at −80
°C. For histological analysis, embedded hearts were sectioned
into 8 μm thick slices with the Leica CM1520 Cryostat and immunostained
with isolectin-B4 (Vector Laboratories FL-1201) for capillary assessment,
WGA (Vector Laboratories, Rhodamine-labeled RL10225) for hypertrophy
assessment, and alpha-SMA (Cy3-labeled C6198 Millipore Sigma) or SM-MHC-11
(Ab50967, Abcam; Alexa Fluor 647, 560400, BD Biosciences) for arteriole
and vessel assessment. The sections were also stained with picrosirius
red connective tissue stain (Ab150681, Abcam) to assess myocardial
fibrosis. All stained sections were imaged by the Cancer Tissue Pathology
Core (Winship Cancer Institute) at 20× immunofluorescence or
bright-field microscopy, as required.

Bheri S., Brown M.E., Park H.J., Brazhkina O., Takaesu F, & Davis M.E. (2023). Customized Loading of microRNA-126 to Small Extracellular Vesicle-Derived Vehicles Improves Cardiac Function after Myocardial Infarction. ACS Nano, 17(20), 19613-19624.

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Immunostaining of Retinal Flat Mounts

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Eyeballs were harvested from mice that were transcardially perfused with 4% paraformaldehyde and then prepared for RPE–choroid flat mounts or cryosections. The antibodies used for staining were anti-isolectin (FL-1201, 1:500; Vector Labs), anti-F4/80 (ab6640, 1:500; Abcam), anti-SULT2B1 (ab254616, 1:500; Abcam), anti-YM1 (1404, 1:500; Stem Cell), anti-ARG1 (16001-1-AP, 1:500; Proteintech), anti-LXRα (ab176323, 1:100; Abcam), anti-LXRβ (ab28479, 1:200; Abcam), anti-ABCA1 (ab18180, 1:200; Abcam), anti-ABCG1 (ab52617, 1:100; Abcam), and anti-STS (17870-1-AP, 1:500; Proteintech). Images were visualized by a fluorescence microscope (Olympus). ImageJ (National Institutes of Health, Bethesda, MD, USA) was used for analysis and quantification.

Wang Y., Liu Y., Wang Y., Wu Y., Chen Z., Wang F., Wan X., Wang F, & Sun X. (2023). Macrophage Sult2b1 promotes pathological neovascularization in age-related macular degeneration. Life Science Alliance, 6(11), e202302020.

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Cardiac Tissue Preparation and Immunostaining

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Processed frozen and paraffin sections were used for downstream analyses. For frozen sections, after fixation, hearts were dehydrated in 15 and 30% sucrose gradients and then embedded into optimal cutting temperature compound (catalog no. 25608-930, VWR International, Radnor, PA, USA) for sectioning. Slides were sectioned at 10-μm intervals for immunofluorescence staining. For paraffin sections, samples were deparaffinized and rehydrated, treated with 3% H₂O₂ in EtOH, treated with antigen retrieval solution (Vector Laboratories Inc., Burlingame, CA, USA), blocked with 10% donkey serum in phosphate-buffered saline, and then incubated with primary antibodies. Antibodies were as follows: goat anti-GFP (ab6673, Abcam, Cambridge, UK), rabbit anti-Yap (NB110-58358, Novus Biologicals, Littleton, CO, USA), rat anti-pHH3 (ab10543, Abcam), rabbit antisarcomeric actinin (ab9465, Abcam), mouse anti-CX43 (sc-13558, Santa Cruz Biotechnology Inc., Dallas, TX, USA), rabbit anti-CD45 (Abcam ab10559), rabbit anti-Aurora kinase B (Abcam ab2254), and isolectin B4 (FL-1201, Vector Laboratories).

Liu S., Li K., Florencio L.W., Tang L., Heallen T.R., Leach J.P., Wang Y., Grisanti F., Willerson J.T., Perin E.C., Zhang S, & Martin J.F. (2021). Gene therapy knockdown of Hippo signaling induces cardiomyocyte renewal in pigs after myocardial infarction. Science translational medicine, 13(600), eabd6892.

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Immunohistochemical Analysis of Retinal Cells

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FVMs and OIR mice retinas were embedded in paraffin and sliced to 3 µm sections. After removal of the paraffin, we rehydrated, blocked and incubated the sections with primary antibodies (Supplementary Table) for 24 h at 4 °C. For immunohistochemistry, the bound antibodies were visualized by an avidin-biotin peroxidase procedure with 3,3′-diaminobenzidine (415172; Nichirei) as the substrate. Counterstaining of nuclei was performed using hematoxylin and eosin procedure. For immunofluorescence analysis, the fluorescence-conjugate secondary antibodies (Supplementary Table) were added for an hour at room temperature and subsequent staining of fluorescein-labeled isolectin-B4 (IB4) (FL-1201; VECTOR laboratories) for 90 minutes at room temperature was performed in FVM sections. Nuclei were counterstained with Hoechst33342 (H3570; Invitrogen). The specimens were mounted in mounting medium (TA030FM; Thermo Fisher) and examined with a microscope (BZ-X800: Keyence).

Kubo Y., Ishikawa K., Mori K., Kobayashi Y., Nakama T., Arima M., Nakao S., Hisatomi T., Haruta M., Sonoda K.H, & Yoshida S. (2020). Periostin and tenascin-C interaction promotes angiogenesis in ischemic proliferative retinopathy. Scientific Reports, 10, 9299.

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Adipose, Liver, and Brain Tissue Harvest

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After 16 weeks of HFD, mice were deeply anesthetized (using a mixture of ketamine hydrochloride [75 mg/kg body weight] and xylazine hydrochloride [15 mg/kg body weight)] in 0.9% sodium chloride solution), and whole blood was taken by cardiac puncture to measure fasting glucose levels, as above. The rest was allowed to clot for 30 min at room temperature followed by serum preparation. Mice were killed by cervical dislocation under deep anesthesia. Their brain, visceral perigonadal adipose tissue (VAT) and liver were removed, weighed and prepared for subsequent molecular or histological analyses, or endothelial cell isolation. In some mice, rhodamine-labeled leptin (FR-003-13, Phoenix Pharmaceuticals; 5 mg/kg body weight) was injected 45 min before tissue harvest via intraperitoneal (i.p.) injection. Then, fluorescein-labeled Lectin I from Griffonia Simplicifolia (FL-1201, Vector Laboratories) was injected intracardially (i.c.) 15 min before tissue harvest to label functional blood vessel and microglial cells in vivo. Tissues were prepared for cryo-preservation and fluorescence microscopy analysis.

Gogiraju R., Witzler C., Shahneh F., Hubert A., Renner L., Bochenek M.L., Zifkos K., Becker C., Thati M, & Schäfer K. (2023). Deletion of endothelial leptin receptors in mice promotes diet-induced obesity. Scientific Reports, 13, 8276.

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Capillary Density Quantification in Myocardial Tissue

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To detect capillaries, slides were incubated with fluorescein-labeled Isolectin B4 (FL-1201, Vector Labs) for 3 h at 37°C and washed 3 times in 1× PBS. As an added measure to facilitate capillary identification, slides were also counterstained with rhodamine-conjugated wheat germ agglutinin (WGA) (RL-1022, Vector) for 30 min at room temperature and washed in 1× PBS (3 times). Tissue sections were then counterstained with DAPI, autofluourescence quenched with 0.1% Sudan Black solution, and mounted under glass coverslips. Images were randomly collected from regions corresponding to infarct, border, and remote myocardial zones. Myocardial regions exhibiting significant morphologic heterogeneity (i.e., infarct zones) were imaged with near complete coverage—acquiring approximately 60-80 images per infarct zone; compared to regions which were more uniform in nature, namely border and remote regions, wherein 6-8 images and 4-6 images were collected and enumerated for each myocardial tissue section, respectively. Capillary density (for each interrogated region) is expressed as the average number of capillaries per unit area (mm²) ± SEM.

Moore JB I.V., Tang X.L., Zhao J., Fischer A.G., Wu W.J., Uchida S., Gumpert A.M., Stowers H., Wysoczynski M, & Bolli R. (2018). Epigenetically Modified Cardiac Mesenchymal Stromal Cells Limit Myocardial Fibrosis and Promote Functional Recovery in a Model of Chronic Ischemic Cardiomyopathy. Basic research in cardiology, 114(1), 3.

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