Mouse egf

Low-passage (P16) 293T cells purchased from the American Type Culture Collection (ATCC, Manassas, VA) were used for the production of lentivirus particles. The MS1 (mouse pancreatic islet) endothelial cell line was also purchased from the ATCC. Immortalized H5V (mouse heart endothelial cells) were previously acquired (23 (link)). 293T, MS1, and H5V cell lines were grown in RPMI supplemented with 10% FBS, 100 IU mL⁻¹ penicillin, and 100 μg mL⁻¹ streptomycin sulfate. HMEC-1 cells, an SV40 transformed human microvascular endothelial cell line (24 (link)), were obtained from the Centers for Disease Control and Prevention (Atlanta, GA) and grown in MCDB 131 media (Invitrogen, Carlsbad, CA) containing mouse EGF (BD, Franklin Lakes, NJ), hydrocortisone (Sigma-Aldrich, St. Louis, MO), 10% FBS, 100 IU mL⁻¹ penicillin, and 100 μg mL⁻¹ streptomycin sulfate and 1x GlutaMax (Invitrogen, Carlsbad, CA). All cell lines used were tested by the University of Pennsylvania Cell Center (Philadelphia, PA) and found to be mycoplasma-free. No other authentication assay was performed.

HMVEC cells (Lonza) were expanded and used from passage 3–7. Endothelial cells were grown using EGM-2MV media (Lonza) on cell culture plates coated with 0.2% gelatin. HMEC-1 cells, an SV40 transformed human microvascular endothelial cell line ^{46 (link)}, were obtained from the Centers for Disease Control and Prevention (Atlanta, GA) and grown in MCDB 131 media (Invitrogen) containing mouse EGF (BD), hydrocortisone (Sigma), 10% FBS, 1% PenStrep and 1× GlutaMax (Invitrogen). Ovarian cancer cell lines were obtained from either from ATCC or were a gift from the laboratory of Carl June developed from primary ovarian tumors. All lines were grown in RPMI containing 10% FBS and 1% PenStrep.