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Mouse anti mef2d

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Mouse-anti-MEF2D is a primary antibody that binds to the transcription factor MEF2D (Myocyte Enhancer Factor 2D). It is designed for use in various immunoassays and research applications.

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Lab products found in correlation

Mouse anti gfap, by Merck Group (1 mentions) Rat anti cd68, by Bio-Rad (1 mentions) Mouse anti map2, by Merck Group (1 mentions) Rabbit anti nse, by Abcam (1 mentions) Rabbit anti caspase 3, by Cell Signaling Technology (1 mentions) Rabbit anti actin, by Merck Group (1 mentions) Hoechst dye, by Merck Group (1 mentions) Mouse anti gephyrin, by Synaptic Systems (1 mentions) Enhanced chemiluminescence system, by Thermo Fisher Scientific (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions)

3 protocols using mouse anti mef2d

1

Immunohistochemistry Antibody Protocol

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Goat-Alexa Fluor® 488 and 594 conjugated anti-mouse or anti-rabbit secondary antibodies were used (Molecular Probes, Eugene, USA). Primary antibodies included rat-anti-CD68 (Serotec, Edinburgh, UK), rabbit-anti-actin, mouse-anti-GFAP, mouse-anti-MAP-2 (Sigma Aldrich, Steinheim, Germany), mouse-anti-huntingtin (Millipore, Schwalbach, Germany), rabbit-anti-LC3 and rabbit-anti-p62 (Enzo Life Sciences, Lörrach, Germany), rat-anti-LAMP-2 (Abl93) and rat-anti-LAMP-1 (1D4B) (DSHB, Iowa City, US), rabbit-anti-LAMP-2A (Pineda, Berlin, Germany), rabbit-anti-cathepsin D (a kind gift from Prof. J. Aerts), mouse-anti-MEF2D (BD Biosciences (Heidelberg, Germany), rabbit-anti-GAPDH and rabbit-anti-α-synuclein (C-20) (Santa Cruz, Dallas, US), rabbit-anti-caspase-3, rabbit-anti-phospho-PRAS40 and rabbit-anti-PRAS40 (Cell Signalling, Frankfurt am Main, Germany) and rabbit-anti-NSE (Abcam, Cambridge, UK).
Rothaug M., Stroobants S., Schweizer M., Peters J., Zunke F., Allerding M., D’Hooge R., Saftig P, & Blanz J. (2015). LAMP-2 deficiency leads to hippocampal dysfunction but normal clearance of neuronal substrates of chaperone-mediated autophagy in a mouse model for Danon disease. Acta Neuropathologica Communications, 3, 6.
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2

Immunocytochemistry of Cultured Mouse Neurons

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Embryonic mouse cortical neurons were cultured on PDL/laminin coated glass coverslips (Bellco) and fixed in 4% paraformaldehyde at room temperature for 10mins. Neurons were blocked in 10% normal goat serum and permeabilized in 0.3% Triton X-100 prior to antibody incubation. Coverslips were incubated in primary antibodies overnight at 4°C. Secondary antibodies were incubated at room temperature for 1 hr. Hoechst dye (0.1μg/ml, Sigma) was used to label nuclei. Primary antibodies used in this study for immunocytochemistry were mouse anti-MEF2D, 1:1000 (BD Biosciences; #610774). mouse anti-Gephyrin, 1:500 (Synaptic Systems; 147021), rabbit anti-GAD65, 1:500 (Millpore; AB5082), chicken anti-GFP, 1:2000 (Millpore; AB16901).
Michelle R.L., Liang-Fu C., Deng J.V., Finn C., Pfenning A.R., Sabhlok A., Wilson K.M, & West A.E. (2016). The transcription factor CaRF limits NMDAR-dependent transcription in the developing brain. Journal of neurochemistry, 137(2), 164-176.
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3

Western Blot Analysis of Myogenic Proteins

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Cells were lysed in lysis buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 0.5% Tween-20, 0.5% NP-40 and protease inhibitors) on ice for 30 min. The supernatant was centrifuged for 30 min at 13500 rpm and 4 °C, ran on SDS–PAGE and then transferred to nitrocellulose membrane. The membranes were blocked with 5% non-fat milk for 1 h at room temperature and then incubated with anti-MyHC (Upstate, 05-716), anti-GAPDH (Cell signaling technology, 2118) antibodies, mouse anti-Mef2d (BD, 610774) at 4 °C overnight. Finally, secondary horseradish peroxidase-labeled antibody was added and incubated for 1 h at room temperature. Signals were detected with an enhanced chemiluminescence system (Thermo Scientific) and visualized by image analyzer.
Fu X., Li S., Jia M., Xu B., Yang L., Ma R., Cheng H., Yang W, & Hu P. (2022). Myogenesis controlled by a long non-coding RNA 1700113A16RIK and post-transcriptional regulation. Cell Regeneration, 11, 13.
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