Nci h69

Human small cell carcinoma cell lines (NCI-H69; ATCC#HTB-119 and NCI-H82; ATCC#HTB-171) and human adenocarcinoma cell lines (PC9; RIKEN BioResource Center#RCB4455) were originally purchased from ATCC or RIKEN BioResource Center and stocked at our institution. The CAFs were obtained from surgically resected small cell carcinoma specimens (Supplementary Table 1), and were cultured according to a previously described method. [28 (link), 29 (link)] NCI-H69 and NCI-H82 were cultured in RPMI1640 (SIGMA-Aldrich, MO) containing 10% fetal bovine serum (FBS; Nichirei Bioscience, Japan) and 1% penicillin and streptomycin (SIGMA-Aldrich). The CAFs were cultured in MEM alpha (Life Technologies Corporation [Gibco], CA) supplemented with 10% FBS and 1% penicillin and streptomycin.

The human SCLC cell line SBC3 was gifted by Dr. Takashi Kijima (Osaka University, Japan), and SBC5 was obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan. BEAS-2B, DMS273, DMS53, NCI-H82, NCI-H526, NCI-H69 and Colo668 were originally obtained from ATCC (Rockville, MD, USA). All the cell lines were routinely maintained and cultured in standard culture conditions, in a CO₂ incubator at 37 °C, and regularly validated using STR profiling and checked for mycoplasma infections prior to experiments. For routine maintenance, SBC3, SBC5, NCI-H82, NCI-H69, NCI-H526, DMS273, DMS53, and Colo668 cells were cultured in RPMI-1640 medium supplemented with 1500 mg/L sodium bicarbonate, 10% fetal bovine serum (Sigma Cat#12303C), 1% penicillin/streptomycin cocktail solution (Invitrogen Cat#15,140–122), 1% sodium pyruvate (Invitrogen Cat#11,360,070), and 1% L-glutamine (Invitrogen Cat#25,030–081).

Neuroblastoma cell lines: IMR32, SKNBE2, SKNAS, SKNSH and SHSY5Y were a kind gift from Marie Arsenian Henriksson’s lab. CHP212 and NB1 were a kind gift from Susanne Schlisio’s lab.
SCLC cell lines (NCIH82, NCIH69) and NSCLC cell lines (NCIH2030, NCIH358 and HCC44) were purchased from the ATCC. Except for the spheroid experiment in Fig. 3D, NCIH82 cells were adapted to grow as monolayers. Single tandem repeat analysis conducted by Public Health England verified the integrity of the NCIH82 cells adapted to grow as monolayers. Profile match was 96%. Human normal dermal fibroblasts (HNDF) were purchased from PromoCell.
IMR32 cells were grown in Nutrient mixture F12 (Sigma-Aldrich #N6658) + Minimum Essential Medium Eagle (Sigma-Aldrich #M4655) (1:1), FBS 10% (Nordic biolabs #SV30160.03HI), Pen-Strep 1% and Non-essential amino acids 1%. SKNBE2, SKNAS, SKNSH and SHSY5Y were grown in 50% DMEM (high glucose) and 50% F12 medium from Sigma-Aldrich supplemented with 10% FBS and 1% penicillin/streptomycin. CHP212 and NB1 were grown in DMEM medium from Sigma-Aldrich (high glucose) supplemented with 10% FBS and 1% penicillin/streptomycin. NCIH82, NCIH69, NCIH2030, NCIH358 and HCC44 were grown in RPMI medium (Sigma-Aldrich #R8758) supplemented with 10% iFBS and 1% penicillin/streptomycin. All cells were grown at 37 °C and 5% CO₂.