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Mouse il 1β duoset elisa kit

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The Mouse IL-1β DuoSet ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse interleukin-1 beta (IL-1β) in cell culture supernates, serum, and plasma samples.

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Lab products found in correlation

Mouse il 6 duoset elisa kit, by R&D Systems (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Caspase glo 1 inflammasome assay kit, by Promega (1 mentions) Cilengitide, by Selleck Chemicals (1 mentions) Mouse inflammation kit, by BD (1 mentions) Duoset elisa kit, by R&D Systems (1 mentions) Mouse tnf α duoset elisa kit, by R&D Systems (1 mentions) Omniscript rt kit, by Qiagen (1 mentions) Kapa sybr fast qpcr master mix, by Roche (1 mentions) Z yvad fmk, by Abcam (1 mentions) Nec 1, by Merck Group (1 mentions) Z vad fmk, by Abcam (1 mentions) Cellular reactive oxygen species detection kit, by Abcam (1 mentions)

Close spelling variants of this product

Mouse IL-1β DuoSet ELISA IL-1β DuoSet ELISA kit Duoset IL-1β Mouse DuoSet ELISA kits Mouse IL-1β DuoSet ELISA kits IL-1β DuoSet ELISA DuoSet Mouse Mouse ELISA kits

7 protocols using mouse il 1β duoset elisa kit

1

Inflammasome Activation in Microglia

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Microglia isolated from 9-mo-old 5XFAD and OPN-KO.5XFAD mice were seeded into 96-well plates at 6×104 cells/well in a conventional microglia culture medium (DMEM-F12 with 10% fetal bovine serum + 1% penicillin/ streptomycin + 10 ng/mL recombinant mouse M-CSF). To induce inflammasome activation, microglia were primed with 100 ng/mL LPS for 3 h followed by stimulation with 10 μM Aβ1-42 fibrils overnight in the presence or absence of 12.5 μg/mL rmOPN. The αVβ3 inhibitor (Cilengitide, Selleck, 10 μM) was added into culture 1 h before the addition of rmOPN. Microglial intracellular caspase-1 activity was analyzed by the bioluminescent Caspase-Glo® 1 Inflammasome Assay Kit (Promega) per the manufacturer’s instruction. The detection specificity of caspase-1 activity was validated using a selective caspase-1 inhibitor (Ac-YVAD-CHO, 1 μM) included in the kit. Culture medium was collected for quantitative determination of microglial IL-1β production by the mouse IL-1β DuoSet ELISA kit (R&D Systems) according to the manufacturer’s instructions.
Qiu Y., Shen X., Ravid O., Atrakchi D., Rand D., Wight A.E., Kim H.J., Liraz-Zaltsman S., Cooper I., Schnaider Beeri M, & Cantor H. (2023). Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer’s disease. Proceedings of the National Academy of Sciences of the United States of America, 120(6), e2218915120.
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2

Plasma Cytokine Profiling by ELISA

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The levels of TNF-α, IL-1β, and IL-6 in human plasma were measured after the blood experiment described above. The assay was performed by using a human inflammation DuoSet ELISA kit (R&D Systems, Minneapolis, MN) specific for each cytokine, according to the manufacturer’s instructions. Absorbance was measured at a wavelength of 450 nm. Data shown are mean values ± standard error of the mean (SEM) obtained from at least 4 independent experiments, all performed in duplicate. For mouse plasma and peritoneal lavage, IL-1β was assessed using the Mouse IL-1β DuoSet ELISA kit (R&D Systems), whereas TNF-α, IL-6, IL-10, MCP-1, and gamma interferon (IFN-γ) were assessed using a mouse inflammation kit (Becton, Dickinson AB) according to the manufacturer’s instructions.
Dahlman A., Puthia M., Petrlova J., Schmidtchen A, & Petruk G. (2021). Thrombin-Derived C-Terminal Peptide Reduces Candida-Induced Inflammation and Infection In Vitro and In Vivo. Antimicrobial Agents and Chemotherapy, 65(11), e01032-21.
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3

Cytokine Measurement Using ELISA Kits

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The levels of TNF-α, IL-1β, and IL-6 were measured using a mouse TNF-α DuoSet ELISA kit (DY-410; R&D Systems), mouse IL-1β DuoSet ELISA kit (DY-401; R&D Systems), and mouse IL-6 DuoSet ELISA kit. (DY-406; R&D Systems), respectively. All ELISAs were performed following the manufacturer’s instructions.
Hwang Y.S., Jang J.P., Park S.H., Kim A., Jang J.H., Yoon H.R., Yoon S.R., Park J.H., Cho H.J, & Lee H.G. (2022). Ponciri Fructus Immaturus ethanol extract attenuates septic shock through inhibition of the STAT1 signaling pathway. Frontiers in Nutrition, 9, 988309.
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4

Cytokine and DNase-I Quantification

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DNase-I levels were analyzed from blood plasma using the Mouse Deoxyribonuclease I ELISA kit (MyBiosource, USA) following the manufacturer’s protocols. IL-1β and IL-6 levels were analyzed from colon lysates using the Mouse IL-1β DuoSet ELISA kit and Mouse IL-6 DuoSet ELISA kit (R&D Systems, USA) following the manufacturer’s protocols, respectively. All assays were performed in at least duplicates.
Wang C.P., Ko G.R., Lee Y.Y., Park J., Park W., Park T.E., Jin Y., Kim S.N., Lee J.S, & Park C.G. (2024). Polymeric DNase-I nanozymes targeting neutrophil extracellular traps for the treatment of bowel inflammation. Nano Convergence, 11, 6.
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5

Measuring IL-1β in Macrophages and Plasma

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Mouse IL-1β cytokine was measured in macrophage culture supernatants and mouse plasma using mouse IL-1β DuoSet ELISA Kit (R&D Systems) according to the manufacturer's instructions. qPCR Total RNA was isolated from macrophages using Trizol reagent (Thermo Fisher Scientific). cDNA was synthesized with Omniscript RT Kit (Qiagen). The relative mRNA expression level of each target gene was quantified by qPCR using KAPA SYBR fast qPCR master mix (Roche). Data were normalized to GAPDH and expressed relative to control unstimulated macrophages. The sequences of the primers are listed in Table S1.
Meyers A.K., Wang Z., Han W., Zhao Q., Zabalawi M., Duan L., Liu J., Zhang Q., Manne R., Lorenzo F.R., Quinn M., Song Q., Fan D., Lin H., Furdui C.M., Locasale J.W., McCall C.E., & Zhu X. (2021). Pyruvate dehydrogenase kinase supports macrophage NLRP3 inflammasome activation during acute inflammation.
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6

Oxidized LDL Detection and Macrophage Assay

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Hi-TBAR (thiobarbituric acid reactive)–oxidized human LDL (BT-910X) and human LDL (BT-903) were purchased from Biomedical Technologies Inc. Macrophage colony-stimulating factor (M-CSF) and mouse IL-1β DuoSet ELISA kits were purchased from R&D Systems. zYVAD.fmk and zVAD.fmk were purchased from BioVision Inc. and ApexBio, respectively. Nec-1 and DPI were obtained from Sigma-Aldrich. Cellular Reactive Oxygen Species Detection Kit was purchased from Abcam.
Karunakaran D., Geoffrion M., Wei L., Gan W., Richards L., Shangari P., DeKemp E.M., Beanlands R.A., Perisic L., Maegdefessel L., Hedin U., Sad S., Guo L., Kolodgie F.D., Virmani R., Ruddy T, & Rayner K.J. (2016). Targeting macrophage necroptosis for therapeutic and diagnostic interventions in atherosclerosis. Science Advances, 2(7), e1600224.
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7

Quantifying Cytokine Levels

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Serum and tissue homogenate were analyzed for cytokine levels using commercially available mouse IL-1β DuoSet ELISA kits (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions.
Kim H.J., Joe Y., Rah S.Y., Kim S.K., Park S.U., Park J., Kim J., Ryu J., Cho G.J., Surh Y.J., Ryter S.W., Kim U.H, & Chung H.T. (2018). Carbon monoxide-induced TFEB nuclear translocation enhances mitophagy/mitochondrial biogenesis in hepatocytes and ameliorates inflammatory liver injury. Cell Death & Disease, 9(11), 1060.
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