Doxorubicin hydrochloride dox

MCF7 human breast cells were obtained from American Type Culture Collection (ATCC) in Manassas, VA, USA. Cells were cultured in 75 cm tissue culture flasks (Corning) in a humidified atmosphere (5% CO₂) at 37 °C. MCF7 cells were cultured in DMEM high glucose media (Gibco, Grand Island, NY, USA) with 10% FBS and penicillin–streptomycin (Gibco). Doxorubicin hydrochloride (DOX; Pfizer, Manhattan, NY, USA), tamoxifen (TAM; Cayman Chemical, Ann Arbor, MI, USA), APCIN (Sigma), Mad2-Inhibitor 1 (Cayman Chemical), nocodazole (Sigma), and thymidine (Sigma-aldrich cat # t1895) were acquired from the indicated providers. All treatment compounds were reconstituted in dimethylsulfoxide (DMSO). Drug treatments were applied at the concentrations and times as indicated. Flow cytometry was performed as described previously [32 (link)].

Doxorubicin hydrochloride (DOX) was purchased from Pfizer (New York, United States). Lauric acid, 2-(tert-butoxycarbonyloxyimino)-2-phenylacetonitrile (BOC-ON), L-ornithine, 2-iminothiolane, glycerol monostearate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), fetal bovine serum (FBS), penicillin, streptomycin, and trypsin EDTA (0.25%) were all purchased from Sigma-Aldrich (St Louis, USA). Trifluoroacetic acid (CF₃COOH), 1,4-dioxane, 1,6-hexanediol, LiChrosolv® HPLC grade methanol, LiChrosolv® HPLC grade acetonitrile, Tween 80, dichloromethane, mannitol, trifluoroacetic acid (TFA), sodium hydroxide (NaOH), and sodium dihydrogen phosphate (NaH₂PO₄) were all obtained from Merck (Darmstadt, Germany). Dexamethasone was purchased from Kimiadaro (Alborz, Iran). The cDNA synthesis kit and total RNA extraction kit were purchased from BioFACT (South Korea). The T7 transcription kit was purchased from Roboklon (Berlin, Germany). Roswell Park Memorial Institute (RPMI) 1640 medium was purchased from the Beta Gene (Mashhad, Iran). Doubled-distilled water was obtained via the Millipore Milli-Q® water purification system (Massachusetts, United States). All other chemicals and reagents were of pharmaceutical grade and used as received.