G7111a pump

Manufactured by Agilent Technologies

Sourced in United States

The G7111A is a high-performance liquid chromatography (HPLC) pump designed for analytical and preparative applications. It is capable of delivering mobile phases at precise flow rates and stable pressures, ensuring reliable and reproducible separations. The pump features advanced electronic controls and programmable parameters to meet the demands of modern HPLC systems.

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Lab products found in correlation

G7115a dad wr, by Agilent Technologies (2 mentions) Diamonsil c18 column, by Agilent Technologies (2 mentions) Eclipse plus c18 column, by Agilent Technologies (1 mentions) G7114a vwd detector, by Agilent Technologies (1 mentions) 1260 high performance liquid chromatography system, by Agilent Technologies (1 mentions) 1260 infinity 2, by Agilent Technologies (1 mentions) Lichrospher rp 18e column, by Merck Group (1 mentions)

5 protocols using g7111a pump

HPLC Quantitation of Drug Samples

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High performance liquid chromatography (HPLC) analysis was performed for drug quantitation using an Agilent 1260II HPLC system comprised of an Agilent G7111A pump and an Agilent G7114A-VWD detector. Data acquisition and processing were performed using Agilent openlab2.3 software.
Separation was performed on an Agilent Eclipse Plus C18 column (250 × 4.6 mm, 5 μm) maintained at 30 °C. The mobile phase consisted of methanol: acetonitrile: water (35:38:27 v/v/v). The flow rate was 1.0 mL/min and the injection volume was 10 μL. Absorbance was monitored at 235 nm.

Huang S., Huang Z., Fu Z., Shi Y., Dai Q., Tang S., Gu Y., Xu Y., Chen J., Wu X, & Ren F. (2020). A Novel Drug Delivery Carrier Comprised of Nimodipine Drug Solution and a Nanoemulsion: Preparation, Characterization, in vitro, and in vivo Studies. International Journal of Nanomedicine, 15, 1161-1172.

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HPLC Quantification of POD Flavones

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The concentrations of the flavones in the treated POD flavone samples were determined by high-performance liquid chromatography (HPLC) on a 4.6 mm × 250 mm (5 μm) YMC-Pack ODS-AM column using an Agilent G7111A pump and a UV detector at 297 nm. The mobile phase consisted of methanol and 0.1% glacial acetic acid solution (60/40, v/v) with a flow rate of 0.8 mL min⁻¹. The treated POD flavone samples (not lyophilized) were diluted 100 times with 60% methanol and were filtered prior to HPLC analysis. The injection volume and column temperature were 20 μL and 25°C, respectively.

Xia G., Li X., Zhang Z, & Jiang Y. (2021). Effect of food processing on the antioxidant activity of flavones from Polygonatum odoratum (Mill.) Druce. Open Life Sciences, 16(1), 92-101.

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HPLC Analysis of Compound CD-AC

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The CD-AC was determined with an Agilent 1260 high-performance liquid chromatography system (consisting of a G7111A Pump, G7115A DAD WR, G7129A vial sampler) equipped with a Diamonsil C18 column (150 mm by 4.6 mm, 5 μm; Agilent Technologies Inc. CA, USA). The mobile phase was acetonitrile delivered at a flow rate of 0.5 mL/min. The UV detection wavelength was 196 nm, the column temperature was 30 °C, and the injection volume was 10 μL.

Xiong X., Chen Y., Wang Z., Liu H., Le M., Lin C., Wu G., Wang L., Shi X., Jia Y.G, & Zhao Y. (2023). Polymerizable rotaxane hydrogels for three-dimensional printing fabrication of wearable sensors. Nature Communications, 14, 1331.

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Vitamin B Analysis by HPLC

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Vitamins of the B class were analyzed according to the method proposed by Li and Chen [46] (link) using an Agilent 1260 Infinity II liquid chromatograph equipped with a G7129A automatic sample feeder, G7111A pump, and G7115A DAD WR detector. The spectral range for analysis spanned from 210 to 400 nm. Extracts obtained according to the method described in Section 2.6 were subjected to analyses performed using a Lichrospher ® RP-18e column (5 µm, 250 × 4 mm, Merck, Darmstadt, Germany).
Elution was performed at a flow rate of 1 mL/min, utilizing a mobile phase composed of 0.1 M KH 2 PO 4 at pH 7 (phase A) and acetonitrile (phase B). The elution followed a gradient starting at 0 min with 3% B, progressing to 10% B at 15 min, reaching 30% B at 40 min, maintaining 30% B until 45 min, reverting to 3% B at 46 min, and stabilizing at 3% B until 56 min. The chromatographic analysis was conducted at a temperature of 30 • C, and the samples were introduced into the column in a volume of 10 µL.

Čmiková N., Kowalczewski P.Ł., Kmiecik D., Tomczak A., Drożdżyńska A., Ślachciński M., Królak J, & Kačániová M. (2024). Characterization of Selected Microalgae Species as Potential Sources of Nutrients and Antioxidants. Foods (Basel, Switzerland), 13(13).

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Rapamycin Encapsulation Efficiency in PLGA Particles

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To determine the encapsulation efficiency (EE) of rapamycin in PLGA particles, a standard curve was first prepared by adding different concentrations of rapamycin in methanol solution. The concentration of rapamycin was then determined with an Agilent 1260 high-performance liquid chromatography system (consisting of a G7111A Pump, G7115A DAD WR, G7129A vialsampler) equipped with a Diamonsil C18 column (150 mm by 4.6 mm, 5 μm; Agilent Technologies Inc. CA, USA). The mobile phase consisted of methanol and water (80:20, v/v) delivered at a flow rate of 1.0 mL/min. The UV detection wavelength was set to 278 nm. The column temperature was maintained at 40 °C, and the injection volume was 20 µL. The EE and LC was calculated using the following equations,

Ma J.C., Luo T., Feng B., Huang Z., Zhang Y., Huang H., Yang X., Wen J., Bai X, & Cui Z.K. (2023). Exploring the translational potential of PLGA nanoparticles for intra-articular rapamycin delivery in osteoarthritis therapy. Journal of Nanobiotechnology, 21, 361.

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