Dulbecco’s Modified Eagle Medium (DMEM), Fetal bovine serum (FBS), streptomycin and penicillin were obtained from Welgene (Daegu, Republic of Korea). Primers shown in Table 1 were acquired from Bioneer (Daejeon, Republic of Korea). Lipopolysaccharide (LPS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), and dextran sulfate sodium (DSS) were from Sigma-Aldrich (Catalogue# 42867, Molecular weight is 40,000 Da, St. Louis, MA, USA). Antibodies for Western blot, iNOS (#2982), COX-2 (#4842), p-TAK1 (#4531), p-NFκB p65 (#3033), IRAK-1 (#4359), p-IKKα/β (#2697), p-IκBα (#2859), β-actin (#4967), T-ERK (#9102), p-ERK (#9101), T-JNK (#9252), p-JNK (#9251), T-p38 (#9212), p-p38 (#9211), NLRP3 (#15101), HRP-linked antibody (#7074) were all obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies conjugated with fluorescence for flow cytometry analysis, PE-Cy5 anti-CD3 (145-2C11; 553065), PE anti-CD4 (RM4-5; 553049), FITC anti-CD8 (53-6.7; 553031), FITC anti-CD25 (3C7; 558689), FITC anti-CD19 (ID3; 553785) and PerCP-Cy5.5 anti-CD69 (H1.2F3; 561931), and CD3e monoclonal antibody (145-2C11; 553058) were obtained from BD Biosciences (San Diego, CA, USA).
Fitc anti cd8 53 6.7
Manufactured by BD
FITC anti-CD8 (53-6.7) is a fluorescently-labeled monoclonal antibody that binds to the CD8 glycoprotein expressed on the surface of certain T cells. It can be used to detect and quantify CD8+ T cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Cox 2, by Cell Signaling Technology (1 mentions)
P iκbα, by Cell Signaling Technology (1 mentions)
P tak1, by Cell Signaling Technology (1 mentions)
Lipopolysaccharide lps 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazoliumbromide mtt, by Merck Group (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
Dextran sulfate sodium (dss), by Merck Group (1 mentions)
Hrp linked antibody, by Cell Signaling Technology (1 mentions)
P jnk, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
P nf κb p65, by Cell Signaling Technology (1 mentions)
Irak1, by Cell Signaling Technology (1 mentions)
Pe anti cd4 rm4 5, by BD (1 mentions)
Dulbecco s modified eagle medium dmem, by Welgene (1 mentions)
T erk, by Cell Signaling Technology (1 mentions)
T jnk, by Cell Signaling Technology (1 mentions)
P ikkα β, by Cell Signaling Technology (1 mentions)
Penicillin, by Welgene (1 mentions)
Fixable viability dyes efluor 506, by Thermo Fisher Scientific (1 mentions)
2 protocols using fitc anti cd8 53 6.7
1
Molecular Mechanisms of Anti-Inflammatory Effects
2
Profiling T cell subsets by flow cytometry
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Cells were sorted on a BD influx (BD Biosciences). Single-cell suspensions of freshly purified naïve CD8+ T cells and activated CD8+ T cells were incubated with PBS containing 1% FBS and then stained with the indicated antibodies for 30 min on ice. Staining reagents included FITC anti-CD8 (53-6.7) (BD, 553031), eFluor 450 anti-CD44 (IM7) (eBioscience, 48-0441-82), APC anti- CD62L (MEL-14) (eBioscience, 47-0621-82), APC anti-PD-1 (J43) (BD, 562671) and PE anti-TIM-3 (5D12) (BD, 566346). Dead cells and cell aggregates were excluded from analyses by Fixable Viability Dyes eFluor™ 506 (eBioscience, 65-0866-18) and FSC-H/FSC-A characteristics. Gating criteria were as follows: for Tn: CD8+, CD44−, CD62L+; for Teff1 and Teff2: CD8+, PD-1+, TIM-3−; for Tex: CD8+, PD-1+, TIM-3+.
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