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2 protocols using flavin adenine dinucleotide disodium

1

Antioxidant and Cytotoxicity Assays

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Hydrogen peroxide solution, 5-Fluorouracil, trhizome® base, malondialdehyde (MDA), thiobarbituric acid (TBA), 3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyl tetrazolium bromide (MTT), gallic acid, glutathione (GSH, reduced form), 1-chloro-2,4-dinitrobenzene (CDNB), β-nicotinamide adenine dinucleotide (NAD, reduced disodium salt hydrate), flavin adenine dinucleotide disodium (FAD, salt hydrate), 2.6-dichloroindophenol (2.6-DCIP, sodium salt hydrate) and DL-Sulforaphane (SFN) were purchased from Sigma-Aldrich, Madrid, Spain.
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2

Cytotoxicity Assay Protocol with Diverse Chemicals

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Chemicals including 1-chloro-2,4-dinitrobenzene (CDNB), 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT), 2,6-dichloroindophenol (2,6-DCIP, sodium salt hydrate), 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), 5-Fluorouracil (5-FU), acetonitrile (can), Bradford Reagent, cumene hydroperoxide, dimethyl sulfoxide (DMSO), flavin adenine dinucleotide disodium (FAD, salt hydrated), glutathione (GSH, reduced form), hydrogen peroxide (H2O2) solution, β-nicotinamide adenine dinucleotide (NAD, reduced disodium salt hydrated), β -nicotinamide adenine dinucleotide phosphate (NADPH), phosphate buffered saline (PBS), sodium chloride, sodium phosphate (NaH2PO4), sulforaphane (SFN), 0.1% aqueous trifluoroacetic acid (TFA) solution, and Trizma® base were purchased from Sigma-Aldrich (Madrid, Spain). Absolute ethanol, di-sodium hydrogen phosphate anhydrous (Na2HPO4), and hydrochloric acid (HCl) were obtained from Panreac AppliChem (Barcelona, Spain). The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Munich, Germany).
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