Mouse liver tissues were fixed with 4% formaldehyde and used to prepare 5-µm thick paraffin sections that were routinely dewaxed to water before being stained (xylene I and II for 20 min each, absolute ethanol I and II and 75% alcohol for 5 min each, and a wash with water).
We used a Masson staining kit (G1006; Servicebio, Wuhan, China). The sections were immersed in Masson A solution overnight and washed on the second day. After being stained with a mixture of Masson B and C solution for 1 min, the sections were differentiated with 1% hydrochloric acid alcohol and stained with Masson D solution for 6 min, Masson E solution for 1 min, and Masson F solution for 2–30 s, successively. Following this, 1% glacial acetic acid was used for rinsing and final differentiation. Sirius red staining solution (G1018; Servicebio) was used to stain the sections for 8 min.
All sections were dehydrated 2–3 times with absolute ethanol and then treated with xylene for 5 min. After mounting with neutral resin was completed, images were taken using a microscope.
We used a Masson staining kit (G1006; Servicebio, Wuhan, China). The sections were immersed in Masson A solution overnight and washed on the second day. After being stained with a mixture of Masson B and C solution for 1 min, the sections were differentiated with 1% hydrochloric acid alcohol and stained with Masson D solution for 6 min, Masson E solution for 1 min, and Masson F solution for 2–30 s, successively. Following this, 1% glacial acetic acid was used for rinsing and final differentiation. Sirius red staining solution (G1018; Servicebio) was used to stain the sections for 8 min.
All sections were dehydrated 2–3 times with absolute ethanol and then treated with xylene for 5 min. After mounting with neutral resin was completed, images were taken using a microscope.