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2 protocols using mouse mcp 1 ccl2 elisa kit

1

Comprehensive Metabolic and Inflammatory Profiling

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Plasma samples were analyzed for total cholesterol (TG), triglyceride (TC), low density lipoprotein-cholesterol (LDL-C), free fatty acid (FFA), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), monocyte chemoattractant protein 1 (MCP-1), adiponectin and leptin by using Cholesterol Quantitation Kit (Sigma), Triglyceride Quantification Kit (Abcam), HDL and LDL/VLDL Cholesterol Assay Kit (Abcam), Free Fatty Acid Quantification Kit (Abcam), Mouse Tumor Necrosis Factor α ELISA Kit (Sigma), Mouse IL-6 ELISA Kit (Sigma), Mouse MCP-1 / CCL2 ELISA Kit (Sigma), Mouse Adiponectin ELISA Kit (Abcam) and Mouse Leptin ELISA Kit (Abcam) according to the manufacturers’ instructions.
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2

Quantitative CXCL5 and CCL2 Assay

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Serum CXCL5 levels were measured using Quantikine ELISA kit (R&D, Minneapolis, MN, USA) according to the manufacturer's instruction. In brief, mouse serum samples were diluted 1:20 and assayed in duplicate, along with CXCL5 controls and working standards. Upon completion of the assay, the optical density (OD) value of each well was measured by a microplate reader (BioTek, Winooski, VT, USA) at wavelengths of 450 nm and 540 nm. The OD values were corrected by subtracting readings at 540 nm from those at 450 nm. Serum CCL2 concentration was analyzed using a mouse MCP-1/CCL2 ELISA Kit (Sigma) according to the manufacturer's protocol. The concentration of each sample was calculated using an online software (http://elisaanalysis.com).
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