Antibody against p65

Human salusin-β was obtained from Phoenix Pharmaceuticals (Belmont, CA, USA). Cell culture supplies were purchased from Costar (Corning Inc., Cypress, CA, USA). Antibodies against ACAT-1, VCAM-1, GAPDH, Lamin B1 and indicated horseradish peroxidase (HRP) conjugated secondary antibodies were purchased from Santa Cruz Biotechnology Co. (Santa Cruz, CA, USA). Antibody against p65 was obtained from Cell Signaling Technology (Beverly, MA, USA). Antibody against NOX2 was purchased from Abcam (Cambridge, MA, USA). Sequence of human miR155 inhibitor is 5′-ACCCCUAUCACAAUUAGCAUUAA-3′ and the sequence of its control is 5′-CAGUACUUUUGUGUAGUACAA-3′ (GenePharma, Shanghai, China). Bay11-7082, N-acetyl-L-cysteine (NAC), deihydroethidium and the reporter plasmid pGL6-NFκB-Luc for measurement of NFκB activity were obtained from Beyotime Biotechnology (Shanghai, China). Apocynin and dimethyl sulfoxide (DMSO) were obtained from Sigma Chemical (St. Louis, MO, USA).

The HeLa and Caski cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin at 37°C in 5% CO₂ atmosphere. The anti-E-cadherin antibody (#ab15148) and anti-CBX7 antibody (#ab91431) were purchased from Abcam. The antibody against p65 (#4764) was purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-tubulin antibody (#SC5286) was bought from Santa Cruz Biotechnology (Santa Cruz, CA). The second antibodies were purchased from Thermo Scientific. Lipofectamine 3000 was purchased from Invitrogen.