Pmod biomedical image quantification software version 3.1

Prior to acquiring the baseline PET scan, T1-weighted magnetic resonance images were acquired with a 3.0-T Siemens SKYRA scanner. PMOD Biomedical Image Quantification Software (version 3.1; PMOD Technologies, Zurich, Switzerland) was used to define anatomical regions of interest (ROI), including the caudate nucleus, putamen, ventral striatum (VS), ventral pallidum (VP) and globus pallidis (GP) and cerebellum, for each subject. PET scans with the D2R radioligand [¹¹C]raclopride were conducted determine D2R availability using published procedures^{45 (link)}. Two scans were conducted in each monkey: one when monkeys were ethanol- and cocaine-naïve (“baseline”) and another when each monkey reached ~400 mg/kg of total cocaine intake (“400 mg/kg”). Monkeys had not self-administered cocaine or consumed ethanol for at least 24 hours prior to each scan.

Details of brain imaging procedures were published previously (Say et al. 2022 (link)). Briefly, prior to acquiring the baseline PET scan, T1-weighted magnetic resonance images were acquired with a 3.0-T Siemens SKYRA scanner. PMOD Biomedical Image Quantification Software (version 3.1; PMOD Technologies, Zurich, Switzerland) was used to define anatomical regions of interest (ROI), including the caudate nucleus, putamen, ventral striatum (VS), ventral pallidum (VP) and globus pallidus (GP) and cerebellum, for each subject. PET scans with the D2R radioligand [¹¹C]raclopride were conducted determine D2R availability using published procedures (John et al. 2018). Two scans were conducted in each monkey: one when monkeys were ethanol- and cocaine-naïve (reported in Say et al. 2022 (link)) and another after each monkey had completed acquisition of cocaine self-administration and a subsequent generation of a cocaine self-administration dose-effect curve (PET scan presented in the present report). Monkeys had not self-administered cocaine or consumed ethanol for at least 24 hours prior to each scan.