ELISA assay kit (Antibodies-online Inc., Atlanta, GA) was used to measure LTβ levels in CMs. Before use, an aliquot of conditioned medium was appropriately diluted with buffers recommended by the manufacturer. Optical density (OD) was measured on a Plate Chameleon V plate reader (Hidex, Finland) at an absorbance of 450 nm. Protein concentration was determined after correcting for optical imperfections in the plate by subtracting OD values at 540/570 nm from OD values obtained at 450 nm.
Elisa assay kit
Manufactured by Antibodies-online
The ELISA assay kit is a laboratory tool used to detect and quantify specific proteins or other analytes in a sample. It employs an enzyme-linked immunosorbent assay (ELISA) technique to measure the target analyte through a series of antigen-antibody reactions and color change reactions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using elisa assay kit
1
Quantifying LTβ Levels in Conditioned Media
2
Quantifying LTβ Levels in Conditioned Media
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