Human c peptide elisa

Manufactured by Mercodia

The Human C-peptide ELISA is a laboratory test kit used to measure the concentration of C-peptide, a byproduct of insulin production, in human blood samples. It provides a quantitative analysis of C-peptide levels without interpretation or extrapolation on intended use.

Automatically generated - may contain errors

Lab products found in correlation

Human ultrasensitive c peptide elisa, by Mercodia (1 mentions) Human insulin elisa, by Mercodia (1 mentions) Onetouch ultra2, by LifeScan (1 mentions)

Close spelling variants of this product

Human C-peptide ELISA kit Human Ultrasensitive C-peptide ELISA Ultrasensitive human C-peptide ELISA kit Mercodia C-peptide ELISA C-peptide ELISAs

3 protocols using human c peptide elisa

Glucose-Stimulated Insulin Secretion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

For static glucose-stimulated insulin secretion assays, stage-8 cells (usually 1–2 clusters, equivalent to ~0.2–0.4 million cells in total), or 15 human islets were rinsed twice with Krebs buffer (129 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl₂, 1.2 mM MgSO₂, 1 mM Na₂HPO₄, 1.2 mM KH₂PO₄, 5 mM NaHCO₃, 10 mM HEPES, and 0.1% BSA in deionized water and sterile filtered) and then pre-incubated in Krebs buffer for 60 min. Cells were then incubated in Krebs buffer containing 3.3 mM glucose for 60 min. The cells were then transferred to a new plate containing Krebs buffer with 16.7 mM glucose (or other reagents) for 60 min. Supernatant samples were collected after each incubation period and frozen for ELISA analysis. ELISA kits included human C-peptide ELISA (#10-1141-01; Mercodia).

Liu H., Li R., Liao H.K., Min Z., Wang C., Yu Y., Shi L., Dan J., Hayek A., Martinez Martinez L., Nuñez Delicado E, & Izpisua Belmonte J.C. (2021). Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells. Nature Communications, 12, 3330.

+ Open protocol

+ Expand

Pancreatic Islet Insulin Secretion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

First, cells of a prefixed number were collected by a cell sorter (differentiated cell clusters: 1 × 10⁶ cells, Human pancreatic islets: 4 × 10⁵ cells). The cells were clustered in a shaking culture and used for the experiment. Human pancreatic islets or differentiated cell clusters from iPS cells were washed twice with Krebs–ringer solution and then pre-incubated for 2 h in low glucose (2.8 mM). The cell clusters were washed twice with glucose-free Krebs–ringer solution, incubated in low glucose Krebs–ringer solution for 1 h, and supernatants were removed. The cells were then incubated in 1 ml of low glucose medium for 45 min, and supernatants were collected, followed by incubated in 1 ml of high glucose (28 mM) Krebs–ringer solution for 45 min and the supernatant was collected. In some cases, cells were again incubated in a low glucose Krebs–ringer solution for 45 min. Insulin in the supernatants were detected using Human Ultrasensitive C-peptide ELISA and Human C-peptide ELISA (MERCODIA).

Watanabe A., Tanaka A., Koga C., Matsumoto M., Okazaki Y., Kin T, & Miyajima A. (2021). CD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells. Scientific Reports, 11, 9530.

+ Open protocol

+ Expand

Evaluating Glucose Regulation in Islet-Grafted Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animals were weighed 2 − 3 times per week and blood glucose was measured using portable glucometers (OneTouchUltra2; LifeScan, CA). Blood samples (~100 μL) for hormone measurements during glucose challenge were collected from the tail vein into tubes containing K2 EDTA, and immediately supplemented with 5 μL aprotinin (10000 KIU/ml). Given the time constraints of bleeding mice through the tail vein, glycemia was not measured during these challenges which were conducted specifically for the purpose of hormone measurements. Plasma insulin levels were measured with Human insulin ELISA (Mercodia, Uppsala, Sweden; 10-1113-01, normal range). C-peptide levels were measured with Human C-Peptide ELISA (Mercodia, 10-1136-01). Enucleation (i.e., removal of eyes bearing the islet graft) was approved by the University of Miami IACUC and was performed under general anesthesia. The eyes were carefully resected and the orbit was packed with sterile gauze saturated with neomycin ointment to prevent bleeding. Glycemia during the first 20–30 mins was measured while the mouse is still under anesthesia and without thereafter. The mice were euthanized immediately after the last glycemia measurement.

Abdulreda M.H., Rodriguez-Diaz R., Caicedo A, & Berggren P.O. (2016). Liraglutide compromises pancreatic beta cell function in a humanized mouse model. Cell metabolism, 23(3), 541-546.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!