Rabbit anti glur1

Manufactured by Merck Group

Sourced in United States

Rabbit anti-GluR1 is a primary antibody that specifically recognizes the GluR1 subunit of the AMPA-type glutamate receptor. It is a laboratory tool commonly used in research applications to detect and study the expression and distribution of the GluR1 receptor in various biological samples.

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GluR1 (11 citations) Anti-GluR1 (10 citations) Rabbit anti- (2 citations)

4 protocols using rabbit anti glur1

Immunostaining of Synaptic Proteins

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Cells (10–13 DIV) were fixed with 4% PFA in 20% sucrose, as previously described65 (link). Staining was performed using mouse anti-synapsin1 (1:300; BD Bioscience, CA, USA), rabbit anti-GluR1 (1:300; Merck, KGaA, Germany) and Oyster-650 conjugated mouse anti-synaptophysin (1:200; Synaptic Systems GmbH, Germany). Secondary antibodies were Dylight594-labeled goat anti-rabbit and Dylight488-labeled goat anti-mouse (1:800, Jackson ImmunoResearch Laboratories, Inc., PA, USA). Pictures were captured using a Nikon A1R MP confocal microscope with a 60x/1.49 NA Apo-TIRF objective.

Koppensteiner P., Trinchese F., Fà M., Puzzo D., Gulisano W., Yan S., Poussin A., Liu S., Orozco I., Dale E., Teich A.F., Palmeri A., Ninan I., Boehm S, & Arancio O. (2016). Time-dependent reversal of synaptic plasticity induced by physiological concentrations of oligomeric Aβ42: an early index of Alzheimer’s disease. Scientific Reports, 6, 32553.

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Immunostaining Protocol for iMNs

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iMNs were fixed in 4% paraformaldehyde (PFA) for 1h at 4 ºC, permeabilized with 0.5% PBS-T overnight at 4 ºC, blocked with 10% FBS in 0.1% PBS-T at room temperature for 2 h, and incubated with primary antibodies at 4 ºC overnight. Cells were then washed with 0.1% PBS-T and incubated with Alexa Fluor® secondary antibodies (Life Technologies) in blocking buffer for 2 h at room temperature. To visualize nuclei, cells were stained with DAPI (Life Technologies) then mounted on slides with Vectashield® (Vector Labs). Images were acquired on an LSM 780 confocal microcope (Zeiss). The following primary antibodies were used: mouse anti-HB9 (Developmental Studies Hybridoma Bank); mouse anti-TUJ1 (EMD Millipore); rabbit anti-VACHT (Sigma); rabbit anti-C9ORF72 (Sigma-Aldrich); mouse anti-EEA1 (BD Biosciences); mouse anti-RAB5 (BD Biosciences); mouse anti-RAB7 (GeneTex); mouse anti-LAMP1 (Abcam); mouse anti-LAMP3 (DSHB, cat. no. H5C6); rabbit anti-LAMP3 (Proteintech, cat. no. 12632); mouse anti-LAMP2 (DSHB, cat. no. H4B4); mouse anti-M6PR (Abcam, cat. no. Ab2733); rabbit anti-GluR1 (EMD Millipore, cat. no. pc246); mouse anti-GluR1 (Santa Cruz); rabbit anti-NR1 (EMD Millipore); mouse anti-NR1 (EMD Millipore, cat. no. MAB363); chicken anti-GFP (GeneTex).

Shi Y., Lin S., Staats K.A., Li Y., Chang W.H., Hung S.T., Hendricks E., Linares G.R., Wang Y., Son E.Y., Wen X., Kisler K., Wilkinson B., Menendez L., Sugawara T., Woolwine P., Huang M., Cowan M.J., Ge B., Koutsodendris N., Sandor K.P., Komberg J., Vangoor V.R., Senthilkumar K., Hennes V., Seah C., Nelson A.R., Cheng T.Y., Lee S.J., August P.R., Chen J.A., Wisniewski N., Victor H.S., Belgard T.G., Zhang A., Coba M., Grunseich C., Ward M.E., van den Berg L.H., Pasterkamp R.J., Trotti D., Zlokovic B.V, & Ichida J.K. (2018). Haploinsufficiency Leads to Neurodegeneration in C9ORF72 ALS/FTD Human Induced Motor Neurons. Nature medicine, 24(3), 313-325.

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Western Blot Analyses of Synaptic Proteins

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For Western blot analyses, the following primary antibodies were used: mouse anti-PSD-95 (1:250; BD transduction), rabbit anti-CDK5 (1:500; Santa Cruz biotechnology, Dallas TX), mouse anti-Akt (1:1000, Cell signal technology, Danvers, MA), rabbit anti-p44/42 MAPK (Erk1/2) (1:1000; Cell signal technology), mouse anti-GFAP (1:500, Sigma Aldrich, Oakville, Ontario), mouse anti-syntaxin (1:10 000, Sigma Aldrich), rabbit anti-GluR1 (1:2000, Millipore, Billrica, MA), mouse anti-GluR2 (1:2000, Millipore), mouse anti-NR1 (1:2000), rabbit anti-D2R (1:500, Millipore) and mouse anti-actin (1:10 000; Millipore). Secondary antibodies IRDye 680 Goat Anti-Rabbit IgG (1:10 000; Mandel Scientific, Guelf, Ontario) or IRDye 800 Goat Anti-Mouse (1:10 000; Mandel Scientific) were then used.
For immunochemistry analysis, mouse monoclonal anti-neuronal nuclei (NeuN) (1:250; Millipore) and mouse monoclonal anti-actin (1:5000; Millipore) were used as primary antibodies. Revelation of labeling using the Odyssey imager was performed using IRDye 800 Goat Anti-mouse IgG (1:1000) and IRDye 680 Goat Anti-mouse IgG (1:1000) secondary antibodies.

Etiévant A., Manta S., Latapy C., Magno L.A., Fecteau S, & Beaulieu J.M. (2015). Repetitive transcranial magnetic stimulation induces long-lasting changes in protein expression and histone acetylation. Scientific Reports, 5, 16873.

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Detailed Immunoblotting and Immunofluorescence Antibodies

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The following antibodies were used for immunoblotting: Mouse anti-calbindin (Sigma), rabbit anti-EAAT2 (Cell Signaling Technology), mouse anti-NeuN (Millipore), Rabbit anti-GluR1 (Millipore), rabbit anti-EAAT4 (Abcam) and goat anti-Lcn2 (R&D Systems). Goat anti-IKK2 (human specific, detects only the transgene), rabbit anti-IKK1/2, rabbit anti-ERK2, rabbit anti-EAAT1, rabbit anti-EAAT3, rabbit anti-galectin 3 (Mac-2), mouse anti-GFAP and all corresponding HRP coupled secondary antibodies were obtained from Santa Cruz Biotechnologies.
Rabbit anti-Prosap1 was kindly provided by Prof. Tobias Böckers (Ulm, Germany).
Goat anti-IKK2, mouse anti-GFAP, mouse anti-NeuN and Mouse anti-calbindin were also used for immunofluorescence. Additionally, following antibodies were used for immunofluorescence: mouse anti-Aldh1l1 (Abcam), rabbit anti-RelA (Santa Cruz Biotechnologies), PE-labeled rat anti-CD11b (eBioscience), and rat anti-CD45, rat anti-CD8, rat anti CD4 (all from BD Biosciences), rabbit anti-Iba1 (Wako) and chicken anti-GFP (Abcam). Corresponding Alexa Fluor-conjugated secondary antibodies were obtained from Molecular Probes (Life Technologies).

Lattke M., Reichel S.N., Magnutzki A., Abaei A., Rasche V., Walther P., Calado D.P., Ferger B., Wirth T, & Baumann B. (2017). Transient IKK2 activation in astrocytes initiates selective non-cell-autonomous neurodegeneration. Molecular Neurodegeneration, 12, 16.

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