Mouse anti cdh1

Manufactured by BD

Sourced in United Kingdom

Mouse anti-CDH1 is a laboratory reagent used for the detection and analysis of the CDH1 protein in various biological samples. CDH1, also known as E-cadherin, is a cell-cell adhesion molecule that plays a crucial role in maintaining cell-cell interactions and epithelial tissue integrity. The mouse anti-CDH1 antibody can be used in applications such as Western blotting, immunohistochemistry, and flow cytometry to investigate the expression and localization of the CDH1 protein.

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Lab products found in correlation

Dylight 488 conjugated goat anti mouse igg, by Jackson ImmunoResearch (3 mentions) Lsm 700 confocal microscope, by Zeiss (2 mentions) Rabbit anti cdh1, by Cell Signaling Technology (2 mentions) Dylight 549 conjugated goat anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Biotin conjugated goat anti rabbit igg ba 1000, by Vector Laboratories (2 mentions) Goat anti mouse af546, by Thermo Fisher Scientific (1 mentions) Axiocam icc1 camera, by Zeiss (1 mentions) Stemi 2000 c, by Zeiss (1 mentions) Zen 2012, by Zeiss (1 mentions) Pgem t easy vector, by Promega (1 mentions) Dylight 594 conjugated goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Rabbit anti dnmt1, by Cell Signaling Technology (1 mentions) Bz x700, by Keyence (1 mentions) Chicken anti gfp, by Thermo Fisher Scientific (1 mentions) Anti mouse alexa 488, by Thermo Fisher Scientific (1 mentions) Anti mouse cy3, by Thermo Fisher Scientific (1 mentions) Anti chicken alexa488, by Thermo Fisher Scientific (1 mentions) Mouse anti gfp, by Thermo Fisher Scientific (1 mentions) Mouse anti gapdh, by Thermo Fisher Scientific (1 mentions) Irdye 700 goat anti rabbit igg, by LI COR (1 mentions)

8 protocols using mouse anti cdh1

In Situ Hybridization and Immunostaining Protocol

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The protocol of ISH was previously described (16 (link)). Specific regions of lefty1 and foxj1a were cloned into pGEM T easy vector (Promega). ISH probe vectors for cmlc1, dand5, spaw, and pan-akap12 were previously described (12 (link), 16 (link)) and primer sequences of lefty1 and foxj1a for ISH probe vectors are summarized in Supplementary Table S1. The protocol of whole-mount immunostaining for Tg(sox17:egfp) embryos was described previously (16 (link)). Mouse anti-Cdh1 (1:200, BD Biosciences) and goat anti-mouse AF546 (1:1000, Invitrogen) were used for immunofluorescence. Stained embryos were mounted in glycerol and images were obtained by an AxioCam ICC-1 camera (Zeiss) on a Stemi 2000C (Zeiss) for immunohistochemistry and an LSM700 confocal microscope (Zeiss) for immunofluorescence, respectively, and processed using ZEN 2012 software (Zeiss).

Kim J.G., Kim H.H, & Bae S.J. (2019). Akap12beta supports asymmetric heart development via modulating the Kupffer’s vesicle formation in zebrafish. BMB Reports, 52(8), 526-531.

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Immunohistochemical Analysis of Mouse Embryos

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Mouse embryos and fetuses at indicated time points were fixed in 4% paraformaldehyde overnight and dehydrated through a graded series into 100% methanol for storage at −20 °C. Embryos and fetuses were rehydrated, and immunohistochemistry was performed as previously described (73 (link)). The following primary and secondary antibodies were used: 1:50 rabbit anti-DNMT1 (#5032, Cell Signaling Technologies), 1:200 mouse anti-CDH1 (BD 610181), 1:250 DyLight 488-conjugated goat anti-mouse IgG, and 1:250 DyLight 594-conjugated goat anti-rabbit IgG (#35502 and #35560, Jackson ImmunoResearch). Sections were imaged using a Keyence BZ-X700 (Keyence) fluorescence microscope.

Ulschmid C.M., Sun M.R., Jabbarpour C.R., Steward A.C., Rivera-González K.S., Cao J., Martin A.A., Barnes M., Wicklund L., Madrid A., Papale L.A., Joseph D.B., Vezina C.M., Alisch R.S, & Lipinski R.J. (2024). Disruption of DNA methylation–mediated cranial neural crest proliferation and differentiation causes orofacial clefts in mice. Proceedings of the National Academy of Sciences of the United States of America, 121(3), e2317668121.

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Immunostaining Protocol for Developmental Biology

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Immunostainings were performed essentially as previously described (Hammerschmidt et al., 1996 (link)). Larvae were fixed in EAF (40% ethanol, 5% acetic acid, 4% formaldehyde in PBS) for whole mount immunostainings with mouse anti Cdh1 (BD Biosciences, 610188, 1:200), or 4% PFA (paraformaldehyde) in PBS for immunostainings using the following primary antibodies: mouse anti Cdh1 (for cryosections), mouse anti-GFP (Invitrogen; A10262, 1:300), chicken anti-GFP (Invitrogen, A10262, 1:500), and mouse anti-tp63 BC4A4 (Zytomend, 1:200). Secondary antibodies were anti-mouseCy3, anti-mouseAlexa488, and anti-chickenAlexa488 (all Invitrogen, 1:500).

Hatzold J., Wessendorf H., Pogoda H.M., Bloch W, & Hammerschmidt M. (2021). The Kunitz-type serine protease inhibitor Spint2 is required for cellular cohesion, coordinated cell migration and cell survival during zebrafish hatching gland development. Developmental biology, 476, 148-170.

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Quantification and Immunoblotting of Proteins

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Protein quantification was performed using 1D-SDS-PAGE/SYPRO Ruby protein staining densitometry, as previously described33 (link). For immunoblotting (10 μg), membranes were probed with primary antibodies [mouse anti-CDH1 (BD Biosciences; 1:1000), rabbit anti-MMP1 (Santa Cruz Biotechnology; 1:1000), mouse anti-VIM (Cell signalling; 1:1000), rabbit anti-YBX1 (Abcam; 1:1000), mouse anti –FN1 (Sigma, 1:1000), rabbit anti-LAMA5 (Santa Cruz Biotechnology; 1:1000), rabbit anti-COL12A1 (Santa Cruz Biotechnology; 1:1000), rabbit anti-p44/42 MAPK (ERK 1/2) (Cell signalling; 1:1000), rabbit anti-phospho-p44/42 MAPK (p-ERK 1/2) (Cell Signalling; 1:1000), or mouse anti-GAPDH (Life technologies; 1:12,000)] for 1 hr at RT in TTBS (50 mM Tris, 150 mM NaCl, 0.05% (v/v) Tween 20 in PBS) followed by incubation with the corresponding secondary antibodies (IRDye 800 goat anti-mouse IgG or IRDye 700 goat anti-rabbit IgG (1:15,000, LI-COR Biosciences) for 1 hr at RT in TTBS. Immunoblots were imaged and visualised using the Odyssey Infrared Imaging System, (v3.0, LI-COR Biosciences, Nebraska USA).

Gopal S.K., Greening D.W., Zhu H.J., Simpson R.J, & Mathias R.A. (2016). Transformed MDCK cells secrete elevated MMP1 that generates LAMA5 fragments promoting endothelial cell angiogenesis. Scientific Reports, 6, 28321.

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Comprehensive Immunohistochemical Profiling of Lung Cells

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The following antibodies were used: rat anti-CDH1 (1:200, Santa Cruz, sc-59778); mouse anti-CDH1 (1:100, BD Biosciences, 560062); rabbit anti cleaved caspase-3 (1:600, Cell Signaling Technologies, #9661); goat anti-SCGB1A1 (1:200, Santa Cruz, T-18); rabbit anti-NKX2.1 (1:400, Santa Cruz, H-190); mouse anti-acetyl-α tubulin (1:2000, Sigma, MABT868); mouse anti-FOXJ1 (1:400, Thermo Fisher Scientific, 14-9965-82); rabbit anti-MUC5AC (1:400, Santa Cruz, H-160); rabbit anti-MUC5B (1:400, Novus Biologicals, NBP1-92151); mouse anti-BrdU (1:400, Thermo Fisher Scientific, B35141); hamster anti-PDPN (1:20, DSHB, 8.1.1); rat anti-SCGB3A1 (1:200, R&D Systems, MAB2954); rat anti-SCGB3A2 (1:200, R&D Systems, MAB3465); rabbit anti-Ki67 (1:400, Thermo Fisher Scientific, PA5-19462); goat anti-NGFR (1:200, Santa Cruz, C-20); mouse anti-α-SMA-Cy3 (1:1000, Sigma-Aldrich, C6198); rabbit anti-SOX9 (1:400, Millipore, AB5535MA); goat anti-SOX9 (1:500, R&D Systems, AF3075); rat anti-Cd140a (1:100, Biolegend, 135901); rabbit anti-RFX3 (1:500, Sigma, HPA035689); mouse anti-TRP63 (1:500, Abcam, ab735); rabbit anti-TRP63 (1:100, Cell Signaling Technology, 13109S) and chicken anti-KRT5 (1:400, Biosite, 905901).

Yin W., Liontos A., Koepke J., Ghoul M., Mazzocchi L., Liu X., Lu C., Wu H., Fysikopoulos A., Sountoulidis A., Seeger W., Ruppert C., Günther A., Stainier D.Y, & Samakovlis C. (2022). An essential function for autocrine hedgehog signaling in epithelial proliferation and differentiation in the trachea. Development (Cambridge, England), 149(3), dev199804.

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Immunofluorescent Staining of ISH-Stained Tissues

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Immunofluorescent staining of ISH-stained tissues and paraffin sections was performed as described previously. Primary antibodies were diluted as follows: 1:200 rabbit anti-CDH1 (3195, Cell Signaling Technology, Beverly, MA), 1:250 mouse anti-CDH1 (610181, BD Transduction Laboratories, San Jose, CA), 1:100 rabbit anti-phosphoSMAD1/5/8 (9511, Cell Signaling Technology, Danvers, MA). Secondary antibodies were diluted as follows: 1:250 Dylight 549-conjugated goat anti-rabbit IgG (111-507-003, Jackson ImmunoResearch, West Grove, PA), and 1:250 Dylight 488-conjugated goat anti-mouse IgG (115-487-003, Jackson ImmunoResearch). Immunofluorescently labeled tissues were counterstained with 4’,6-diamidino-2-phenylindole, dilactate (DAPI), and mounted in anti-fade medium (phosphate buffered saline containing 80% glycerol and 0.2% n-propyl gallate). Whole mount immunohistochemistry was performed as described previously. Primary antibody was diluted 1:750 rabbit anti-CDH1 and secondary antibody was diluted 1:500 biotin conjugated goat anti-rabbit IgG (BA-1000, Vector, Burlingame, CA).

Keil K.P., Altmann H.M., Abler L.L., Hernandez L.L, & Vezina C.M. (2015). Histone acetylation regulates prostate ductal morphogenesis through a BMP dependent mechanism. Developmental dynamics : an official publication of the American Association of Anatomists, 244(11), 1404-1414.

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Immunofluorescent Staining of ISH-Stained Tissues

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Immunofluorescent staining of ISH-stained tissues and paraffin sections was performed as described previously (12 (link), 23 (link)). Primary antibodies were diluted as follows: 1:200 rabbit anti-CDH1 (3195, Cell Signaling Technology, Beverly, MA), 1:250 mouse anti-CDH1 (610181, BD Transduction Laboratories, San Jose, CA) 1:50 mouse anti-KRT14 (ms-115-p0, Thermo Fisher Scientific), 1:250 rabbit anti-AR (sc-816, Santa Cruz Biotechnology, Santa Cruz, CA), 1:200 rabbit anti-DNMT1 (5032, Cell Signaling Technology). Secondary antibodies were diluted as follows: 1:250 Dylight 549-conjugated goat anti-rabbit IgG (111-507-003, Jackson ImmunoResearch, West Grove, PA), 1:250 Dylight 488-conjugated goat anti-rabbit IgG (111-487-003, Jackson ImmunoResearch) and 1:250 Dylight 488-conjugated goat anti-mouse IgG (115-487-003, Jackson ImmunoResearch). Immunofluorescently labeled tissues were counterstained with 4’,6-diamidino-2-phenylindole, dilactate (DAPI), and mounted in antifade medium (phosphate buffered saline containing 80% glycerol and 0.2% n-propyl gallate). Whole mount immunohistochemistry was performed as described previously (23 (link)). Primary antibody was diluted 1:750 rabbit anti-CDH1 and secondary antibody was diluted 1:500 biotin conjugated goat anti-rabbit IgG (BA-1000, Vector, Burlingame, CA).

Keil K.P., Abler L.L., Laporta J., Altmann H.M., Yang B., Jarrard D.F., Hernandez L.L, & Vezina C.M. (2014). Androgen receptor DNA methylation regulates the timing and androgen sensitivity of mouse prostate ductal development. Developmental biology, 396(2), 237-245.

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Antibody-Based Immunophenotyping Protocol

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The following antibodies used in this study were purchased from BD PharMingen (San Diego, CA): mouse anti-CD4 PE (H129. 19 ), anti-CD8a PE (53-6.7), anti-CD11b PE (M1/70), anti-F4/80 PE (BM8), and anti-Ly6C FITC (AL-21) monoclonal antibodies. In addition, PE-conjugated anti-mouse CD44, PE rat IgG1, and FITC rat IgG2a isotype control antibodies were purchased from eBioscience (San Diego, CA). The remaining antibodies used were as follows: anti-MST3/STK24 (EP1468Y; Abcam, UK), mouse anti-CDH1 (BD Transduction Laboratories, San Jose, CA), rabbit anti-beta catenin (GeneTex, Inc., San Antonio, TX), rabbit anti-AKT1 and peroxidase-conjugated goat anti-rabbit IgG (Cell Signaling, Boston, MA), mouse antiβ-actin (GeneTex, Inc.), and peroxidase-conjugated sheep anti-mouse IgG (Chemica, San Diego, CA).
Cell culture MKN45 cells were kindly provided by Professor Ming-Derg Lai (National Cheng Kung University, Tainan, Taiwan). The MKN45 cell lines were authenticated in 2013 by DNA short tandem repeat pro ling at Bioresource Collection and Research Center. These cell lines were maintained in RPMI 1640 medium containing 10% fetal bovine serum (FBS) (Gibco, Life Technologies, Grand Island, NY) and 1% penicillin/streptomycin. M12 cells were maintained in Dulbecco's modi ed Eagle's medium/high glucose supplemented with 10% FBS and 1% penicillin/streptomycin.

Chen Y., Wang C., Fang J., & Hsu H. (2021). Serine/threonine-protein kinase 24 is an inhibitor of gastric cancer metastasis.

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