Jsm 5510

Manufactured by JEOL

Sourced in Japan

The JSM-5510 is a scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of materials. It features a tungsten filament electron source, secondary electron and backscattered electron detectors, and a variable pressure capability to examine samples without the need for extensive sample preparation. The JSM-5510 provides reliable performance and versatility for a variety of applications in materials science, life science, and industrial research and development.

Automatically generated - may contain errors

Lab products found in correlation

Jsc 1200, by JEOL (4 mentions) Jfc 1200 fine coater, by JEOL (3 mentions) Jfc 1200, by JEOL (2 mentions) G 306, by Agar Scientific (2 mentions) Carbon tabs 9 mm, by Agar Scientific (2 mentions) 7650 tem, by Hitachi (1 mentions) Escalab 250, by Thermo Fisher Scientific (1 mentions) Nanoscope 5, by Veeco (1 mentions) S 400, by Hitachi (1 mentions) Jsm 5510 sem, by JEOL (1 mentions) D8 advance, by Bruker (1 mentions) Ex 250, by Horiba (1 mentions) Oca 15ec, by Dataphysics (1 mentions) Jem 1200ex, by JEOL (1 mentions) Jsm 7600fa, by JEOL (1 mentions) Jem 2100, by JEOL (1 mentions) Vacuum evaporator, by Edwards Lifesciences (1 mentions) Application suite, by Leica (1 mentions) Scd 004 sputter coater, by Hitachi (1 mentions) Mc170 hd camera, by Leica (1 mentions)

35 protocols using jsm 5510

Leaf Surface and Chloroplast Ultrastructure

Check if the same lab product or an alternative is used in the 5 most similar protocols

The micromorphological characteristics (cuticular and epicuticular waxes) of the leaf adaxial and abaxial surface were studied by SEM. The SEM analysis was performed on pre-herbarium leaves in the air-dry state. Leaf lamina was gold-coated with thickness of 0.12 nm using a vacuum evaporator, Jeol JFC-1200 fine coater. Sample observations were performed using a scanning electron microscope Jeol JSM-5510 (Tokyo, Japan) and at least 15 micrographs of each treatment were analyzed.
Chloroplast ultrastructure was studied by transmission electron microscopy following the procedure described elsewhere [65 (link)]. Briefly, leaf segments (1 mm²) derived from the 2nd and 3rd well-developed leaves were fixed in glutaraldehyde and post-fixed in KMnO₄. The samples were dehydrated in a gradient of ethyl alcohol (25–100%) and embedded in Durcupan (Fluka, Buchs, Switzerland). Ultra-thin cross-sections were examined by means of an electron microscope (JEOL 1200 EX, Tokyo, Japan). At least 15 micrographs from five plants of each treatment were analyzed.

Velikova V., Petrova N., Kovács L., Petrova A., Koleva D., Tsonev T., Taneva S., Petrov P, & Krumova S. (2021). Single-Walled Carbon Nanotubes Modify Leaf Micromorphology, Chloroplast Ultrastructure and Photosynthetic Activity of Pea Plants. International Journal of Molecular Sciences, 22(9), 4878.

+ Open protocol

+ Expand

Characterization of Magnetic Graphene Oxide

Check if the same lab product or an alternative is used in the 5 most similar protocols

The structure and morphology of MRGO were characterized by TEM (TEM-7650, Hitachi, Chiyoda-ku, Japan) at an acceleration voltage of 100 kV. The binding energy characteristics of GO and MRGO were investigated using X-ray photoelectron spectroscopy (XPS) (ESCALAB 250, Thermo VG Scientific, West Sussex, UK), equipped with Mg Kα at 1253.6 eV at the anode. X-ray diffraction (XRD) was conducted using a D₂ Phaser BRUKER X-ray powder diffractometer to evaluate the phase of the material by scanning dried powder in the 2θ range of 20–80° with Cu K_α (1.5406 Å) radiation. The zeta potential of the graphene-based material before and after polymeric modification was determined through electrophoretic mobility measurements (Horiba Instrument). Vibrating sample magnetometry (VSM) (Lakeshore model 7400) was used to evaluate the magnetization of MRGO as a function of the magnetic field (Oe) at room temperature.
The bacteria capture by MRGO was observed using scanning electron microscopy (SEM) (JSM 5510, JEOL, Tokyo, Japan).

Hardiansyah A., Yang M.C., Liao H.L., Cheng Y.W., Destyorini F., Irmawati Y., Liu C.M., Yung M.C., Hsu C.C, & Liu T.Y. (2020). Magnetic Graphene-Based Sheets for Bacteria Capture and Destruction Using a High-Frequency Magnetic Field. Nanomaterials, 10(4), 674.

+ Open protocol

+ Expand

Surface Characterization by Advanced Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ramé-Hart model 290 automated goniometer with DROPimage Advanced v2.4 and WTW inoLab 720 with a conductivity cell Tetracon 325, BAM – Nanofilm_ultrabam (Accurion); Image sizes 720 × 400 micrometers. AFM – Nanoscope V (Veeco Instruments Inc.). Scale bare is indicated. SEM – Jeol scanning electron microscope JSM-5510 (Jeol Ltd.) was used for the observation of dried samples.

Anchev B.A., Tsekova D.S., Mircheva K.M, & Grozev N.A. (2019). Monolayer formed by l-Asp-based gemini surfactants self-assembled in 1D nanostructures. RSC Advances, 9(57), 33071-33079.

+ Open protocol

+ Expand

Biofilm Morphology and Composition Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Scanning electron microscopy for biofilm was performed. The bacterial consortium control (absence of nanoparticles) and treated cells with 1 μg/ml of TiO₂-NPs in waste water medium was grown in 24 well microtitre plates and incubated at 37°C for 24 h. The samples were washed with 1x PBS after 24 h incubation and was fixed with 2.5% glutaraldehyde. The fixation step was further followed by dehydration steps with 20, 40, 60, 80 and 100% ethanol and dried overnight. The glass slides were fixed on the specimen mount with the help of carbon tape. To avoid surface charge interference, gold sputtering was executed in an argon atmosphere. Morphological analysis and surface elemental composition of the biofilm was performed with the help of SEM-EDX (S-400, HITACHI and Tokyo, Japan JEOL JSM-5510) [16 (link)].

Mathur A., Kumari J., Parashar A., T. L., Chandrasekaran N, & Mukherjee A. (2015). Decreased Phototoxic Effects of TiO₂ Nanoparticles in Consortium of Bacterial Isolates from Domestic Waste Water. PLoS ONE, 10(10), e0141301.

+ Open protocol

+ Expand

Synthesis of Stabilized Nanocrystals via Ball Milling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The nanocrystals were prepared using a wet media milling technique with a ball mill, and Tween 80 was used as a stabilizer to prevent aggregation. The stabilizer was dissolved in water at 1% w/w, and the QUE powder was added to the solution and stirred mechanically to ensure adequate wetting. The resulting suspension was introduced into a milling chamber containing zirconium oxide beads, and the milling procedure was conducted at a rotational speed of 300 rpm for four cycles, each consisting of a 5 min milling period followed by a 5 min interval. The nanocrystals were dried and stored in a low-temperature, low-humidity environment until required for subsequent applications. The particle size, zeta potential, and polydispersity index were measured using a Horiba Particle Size Analyzer SZ-100 instrument, and the particle sizes and morphologies of the samples were observed using a scanning electron microscope (JSM–5510-SEM, Make: Jeol, Model: JSM-5510, Tokyo, Japan) [30 (link)].

Lakshmi Y.S., Prasanth D.S., Kumar K.T., Ahmad S.F., Ramanjaneyulu S., Rahul N, & Pasala P.K. (2023). Unravelling the Molecular Mechanisms of a Quercetin Nanocrystal for Treating Potential Parkinson’s Disease in a Rotenone Model: Supporting Evidence of Network Pharmacology and In Silico Data Analysis. Biomedicines, 11(10), 2756.

+ Open protocol

+ Expand

Characterizing Lyophilized SLN Morphology

Check if the same lab product or an alternative is used in the 5 most similar protocols

The shape and surface morphology of lyophilized SLNs were examined utilizing SEM, JSM-5510 (Jeol Ltd, Tokyo, Japan) supplemented with a digital camera. The samples were coated with gold and mounted on a sample holder. The electron micrographs were taken at an accelerating voltage of 5 kV.

Zuhair Alshawwa S., Salah Labib G., Badr-Eldin S.M, & Ahmed Kassem A. (2023). Solid lipid Lyo-Nanosuspension: A promising stabilized oral delivery system for the antihyperglycemic extract of mistletoe Plicosepalus acacia. Saudi Pharmaceutical Journal : SPJ, 31(8), 101689.

+ Open protocol

+ Expand

Scanning Electron Microscopy of Xanthomonas-Infected Leaves

Check if the same lab product or an alternative is used in the 5 most similar protocols

Two types of probes were prepared: 1, from leaves infected with X. euvesicatoria strain 269p, and 2, from leaves infected with X. euvesicatoria 269p and subsequently treated with BsXeu269p/3. The preparation of the specimens was carried out according to a procedure described by Ganeva et al. [46 (link)]. Air-dried 0.5 cm² segments from leaf surfaces of the middle part of the leaf lamina were attached to aluminum specimen stubs by double-sided carbon tape. After being sputter-coated with gold (Jeol JFC-1200 fine coater, Tokyo, Japan), the specimens were observed by scanning electron microscope Jeol JSM-5510.

Shopova E., Brankova L., Ivanov S., Urshev Z., Dimitrova L., Dimitrova M., Hristova P, & Kizheva Y. (2023). Xanthomonas euvesicatoria-Specific Bacteriophage BsXeu269p/3 Reduces the Spread of Bacterial Spot Disease in Pepper Plants. Plants, 12(19), 3348.

+ Open protocol

+ Expand

Microstructural Analysis of Polycrystalline Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Microstructure investigation of polycrystalline samples and crystals was performed with JEOL JSM 5510 (LaB₆ cathode, 30 kV) scanning microscope. Phase identification and lattice parameters determination (for polycrystalline sample and crystals) were performed using room-temperature X-ray powder diffraction data obtained by image plate Guinier camera Huber G670, (CoK_α1 radiation, λ = 1.78892 Å). Crystalline Ge (a = 5.6576 Å, ref. 21 ) was used as internal standard. Ex-situ X-ray diffraction data for polycrystalline electrodes were collected in air using Bruker D8-Advance diffractometer (CuK_α1 radiation, λ = 1.540598 Å, LynxEye PSD) in reflection mode. The electrodes previously were covered by air-protective one side sticky tape.

Alekseeva A.M., Drozhzhin O.A., Dosaev K.A., Antipov E.V., Zakharov K.V., Volkova O.S., Chareev D.A., Vasiliev A.N., Koz C., Schwarz U., Rosner H, & Grin Y. (2016). New superconductor LixFe1+δSe (x ≤ 0.07, Tc up to 44 K) by an electrochemical route. Scientific Reports, 6, 25624.

+ Open protocol

+ Expand

Bread Surface Structural Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bread samples were freeze-dried, mounted on stubs (G 306; 10 mm × 10 mm Diameter; Agar Scientific, Stansted, UK) and fixed using carbon tape (G3357N; Carbon Tabs 9 mm; Agar Scientific, Stansted UK). Mounted bread samples were sputter-coated with a gold-palladium alloy (ratio of 80: 20), using a Polaron E5150 sputter coating unit, and imaging was captured with a JEOL Scanning Electron Microscope (JSM-5510, Jeol Ltd., Tokyo, Japan). Settings for analysis were as follows: 5 kV 185 voltage, 20 mm working distance and a magnification factor of 1000.

Neylon E., Arendt E.K., Zannini E, & Sahin A.W. (2021). Fermentation as a Tool to Revitalise Brewers’ Spent Grain and Elevate Techno-Functional Properties and Nutritional Value in High Fibre Bread. Foods, 10(7), 1639.

+ Open protocol

+ Expand

SEM Analysis of Root Canal Resin Adhesion

Check if the same lab product or an alternative is used in the 5 most similar protocols

The sectioned root specimens were air-dried, dehydrated, desiccated, and sputter-coated with palladium (Desk II Cold Sputter/Etch Unit, Denton Vacuum LLC, Moorestown, NJ). The sputter-coated cross-sectioned root surfaces were imaged under SEM (JSM5510, JEOL USA Inc. Peabody, MA) at 60–90× magnification for an overall view of the entire root canal circumference and at 250× magnification for a site-specific view of an area of resin adhesion on the root canal circumference. Each image was identified with regard to its specimen number, cross-sectional surface region (cervical, middle, and apical), and conditioning group. SEM digital images were then randomly coded and loaded into the VixWin Digital Imaging Software Program (Gendex Dental Systems, Des Plains, IL) for an independent and blind assessment of resin adhesion by four calibrated examiners. Examples of SEM cross-sectional circumferential and site-specific images for each group are shown in Figure 1a-e.

Fiore P.M., Phebus J.G., Himel V.T, & de Rijk W.G. (2017). Methacrylate Resin Adhesion in Root Canals Conditioned with Phosphoric Acid and Ethylenediaminetetraacetic Acid. European Endodontic Journal, 2(1), 1-6.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!