Os workstation

The AB ExionLC system (AB SCIEX, Framingham, MA, USA) coupled to the AB SCIEX API 6500 Qtrap+ system (AB SCIEX) was used to analyze targeted phenolic metabolite profiles. Specifically, the positive and negative electrospray ionization modes and the AB SCIEX OS workstation (version 1.7.1) were used. A Waters UPLC HSS T3 column (1.8 μm, 2.1 × 100 mm) was used in the positive and negative ion modes. The binary gradient elution system consisted of (A) water (containing 0.1% formic acid, v/v) and (B) acetonitrile. The injection volume was 5 μL. Using an electrospray ion source, the analyte was analyzed in the multiple reaction monitoring (MRM) mode for both positive and negative ion scanning, resulting in increased sensitivity. Mass spectrometry parameters, such as the declustering potential and collision energy, were optimized to enable the rapid screening of target compound ion pairs. The optimized mass spectrometry conditions were as follows: positive mode—collision gas: 35, ion spray voltage: 5500 V, ion spray temperature: 600 °C, ion source gas 1: 60, and ion source gas 2: 50; negative mode—collision gas: 35, ion spray voltage: −4500 V, ion spray temperature: 600 °C, ion source gas 1: 60, and ion source gas 2: 50.

The AB ExionLC system (AB SCIEX, Framingham, MA, USA) coupled to the AB SCIEX API 6500 Qtrap+ system (AB SCIEX) was used to analyze targeted phenolic metabolite profiles. Specifically, the positive and negative electrospray ionization modes and the AB SCIEX OS workstation (version 1.7.1) were used. A Waters UPLC HSS T3 column (1.8 μm, 2.1 × 100 mm) was used in the positive and negative ion modes. The binary gradient elution system consisted of (A) water (containing 0.1% formic acid, v/v) and (B) acetonitrile. The injection volume was 5 μL. Using an electrospray ion source, the analyte was analyzed in the multiple reaction monitoring (MRM) mode for both positive and negative ion scanning, resulting in increased sensitivity. Mass spectrometry parameters, such as the declustering potential and collision energy, were optimized to enable the rapid screening of target compound ion pairs. The optimized mass spectrometry conditions were as follows: positive mode—collision gas: 35, ion spray voltage: 5500 V, ion spray temperature: 600 °C, ion source gas 1: 60, and ion source gas 2: 50; negative mode—collision gas: 35, ion spray voltage: −4500 V, ion spray temperature: 600 °C, ion source gas 1: 60, and ion source gas 2: 50.