Anti e cadherin mab

The following primary monoclonal antibodies (mAbs) and polyclonal antibodies (pAbs) were used to detect junctional and signaling proteins by immunofluorescence labeling and immunoblotting: anti-occludin, anti–ZO-1, and anti–E-cadherin mAb (Thermo-Fisher Scientific, Waltham, MA); anti–β-catenin mAb (BD Bioscience, San Jose, CA), Anti-claudin 1 and 4 pAbs (Abcam, Cambridge, UK), anti-cortactin mAb (p80/85 clone 4F11, EMD Millipore, Billerica, MA), anti-cortactin pAb (H222), anti-phospho-CREB (Ser133; 87G3) rabbit mAb, and anti-CREB (48H2) rabbit mAb (Cell Signaling Technologies, Danvers, MA), anti-GAPDH mAb (6C5, Abcam, Cambridge, MA). Fluorescently labeled phalloidin 488 (Thermo-Fisher Scientific) was used to visualize actin filaments. Anti-rabbit and anti-mouse secondary antibodies conjugated to Alexa-488 or Alexa-568 dyes were obtained from Thermo-Fisher Scientific. Mouse and rabbit secondary HRP-conjugated antibodies were purchased from GE Healthcare (Pittsburgh, PA).

The following monoclonal (mAbs) and polyclonal antibodies (pAbs) were used to detect tight junction and adherens junction proteins by immunofluorescent labeling and/or immunoblotting: anti-RSV A2 pAb, which reacts with glycoprotein G of the A2 strain (GTX70381; GeneTex, Irvine, CA), anti-β-catenin mAb, anti-GAPDH mAb (Abcam, Cambridge, MA), anti-zonula occludens (ZO)-1 pAb, anti-occludin mAb, anti-E-cadherin mAb (Thermo-Fisher, Waltham, MA), and cleaved caspase-3 pAb (Cell Signaling, Danvers, MA). Alexa Fluor 488- and 568=conjugated anti-rabbit and anti-mouse secondary antibodies were obtained from Thermo-Fisher Scientific (Waltham, MA). Mouse and rabbit secondary horseradish peroxidase-conjugated antibodies were purchased from GE Healthcare (Pittsburgh, PA). N-acetylcysteine (NAC) was purchased from Sigma.