Approximately 20 mg of each spleen and brain sample from the small mammal was homogenized by adding 200 μL sterilized trypsin soy broth (BD Biosciences, Franklin Lakes, NJ, USA), plated onto two trypsin soy agars containing 5% (vol/vol) defiber sheep blood (BD Biosciences), and incubated at 37 °C in an atmosphere containing 5% CO2. Pure colonies of Bartonella species were obtained according to previous methods22 (link).
Defiber sheep blood
Manufactured by BD
Sourced in United States
Defiber sheep blood is a lab equipment product. It is a processed form of sheep blood, with the fibrin removed. This processing creates a uniform, defibrinated blood material for use in various laboratory procedures and applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using defiber sheep blood
1
Isolating Bartonella from Small Mammal Samples
2
Isolation of Bartonella from Spleens
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Spleens were harvested under sterile conditions from each animal after euthanasia. Approximately 20 mg of each spleen sample was homogenized by adding 200 μL sterilized trypsin soy broth (BD Biosciences, Franklin Lakes, NJ, USA), plated onto two trypsin soy agars containing 5% (vol/vol) defiber sheep blood (BD Biosciences), and incubated at 37 °C in an atmosphere containing 5% CO2. Pure Bartonella colonies were obtained using a protocol described in previous studies19 (link).
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