Plasmin activity was measured by fluorogenic substrate cleavage. Plasmin substrate (Bachem, Fremont, CA, USA) at a concentration of 0.1 mM was used and the cleavage of the substrate due to free plasmin, and plasmin nanoparticles was determined at 370/440 nm excitation and emission using a spectramax M3 plate reader. Nanoformulation activity was compared to that of equivalent plasmin activity with and without anti-plasmin at equimolar concentration.
Plasmin substrate
Manufactured by Bachem
Sourced in United States
Plasmin substrate is a laboratory reagent used in biochemical and biomedical research. It is a synthetic compound that serves as a substrate for the enzyme plasmin, which is involved in the fibrinolytic system. The core function of the plasmin substrate is to provide a standardized and measurable platform for assessing plasmin activity in various experimental settings.
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Lab products found in correlation
2 protocols using plasmin substrate
1
Fluorogenic Plasmin Activity Assay
2
Investigating Plasmin-Mediated Apoptosis
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Human lys-plasmin (HPLM), human fibrinogen, and human thrombin were purchased form Molecular Innovations., Novi, MI. Plasmin substrate was purchased from Bachem, Torrance, CA. The apoptosis and necrosis assay kit was purchased from Biotium, Fremont, CA. Human α2-antiplasmin, and all the other chemicals, were purchased from Millipore Sigma, St. Louis, MO, or Thermo Fisher Scientific.
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