Baff r

All standard culture reagents were obtained from Gibco (Life Technologies), Carlsbad, CA. Puromycin was purchased from Sigma-Aldrich (Shanghai) Trading Co., LTD. FITC-labeled monoclonal antibodies specific to BAFF and B-cell activating factor receptor (BAFF-R) were purchased from eBioscience, USA. Monoclonal antibodies specific to HBe was purchased from Abcam (Hong Kong) Ltd., New Territories, Hong Kong. The reverse transcription polymerase chain reaction (PCR) kit and SYBR green master used for semiquantitative real time-PCR were purchased from Roche Diagnostics Corporation, Roche Applied Science, Indianapolis, IN. BAFF, IL-6, and TNF-α enzyme-linked immunosorbent assay (ELISA) kits were purchased from R&D Systems, Inc., Minneapolis, MN. IL-10 and APRIL ELISA kits were purchased from Abcam. 6.5 mm Transwell^® with 0.4 μm pore polycarbonate membrane inserts was purchased from Corning. Cell Counting Kit-8 was from Dojindo Laboratorise (Shanghai), Co., Ltd. All primers were synthesized and validated by Sangon Biotech (Shanghai, China) Co., Ltd.

Tumors were excised, weighed, crushed, strained through a 0.45-μm filter, and surface stained with: anti-LY6G, LY6C, CD8, NK1.1, CD11b, CD45.2, F4/80, PD-L1, BAFFr, CD5, CD25, IgM, and CD19 antibodies (eBioscience). Intracellular staining for Granzyme B and IFNγ was performed using the Foxp3 mouse Treg Cell Kit (eBioscience). Experiments were performed using a FACS Fortessa and analyzed with FlowJo software.