Mira analyzer

Manufactured by Roche

Sourced in France

The MIRA analyzer is a compact and efficient laboratory instrument designed for clinical chemistry analysis. It offers automated processing of a wide range of clinical chemistry tests, including assays for enzymes, electrolytes, and other analytes. The MIRA analyzer provides reliable and consistent results, making it a valuable tool for clinical laboratories and healthcare settings.

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Lab products found in correlation

Precast mini gels, by Thermo Fisher Scientific (1 mentions) Bodipy labeled ldl, by Thermo Fisher Scientific (1 mentions) Quickchange lightning kit, by Agilent Technologies (1 mentions) Nefa hr kit, by Fujifilm (1 mentions) Glucometer, by Roche (1 mentions)

4 protocols using mira analyzer

Humanized LDLR Mice for Cholesterol Analysis

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Animal studies were performed in accordance with the University of Pennsylvania, institutional review board (IRB). LDLR^-/-, APOBEC-1^-/- double knockout (DKO) and LDLR^-/-, APOBEC-1^-/-, human ApoB100 transgenic (LAHB) mice were injected via tail vein for vector administration and serum collected by retro-orbital bleeds. At end of study animals were sacrificed and livers harvested for analysis. Vector were obtained from the Vector Core at the University of Pennsylvania and expressed cDNAs for hLDLR, hPCSK9 or hIDOL driven from a thyroxine binding globulin promoter (TBG). Mutations were introduced into wild type hLDLR using the Quick Change lightning kit (Agilent). Serum cholesterol levels were analyzed on a MIRA analyzer (Roche). Western blotting was done using precast mini gels (Invitrogen) and probed using a polyclonal hLDLR antibody. In vitro LDLR assay was performed by transiently transfecting HEK293 cells and pulsing the cells the following day with BODIPY labeled LDL (Invitrogen). Data were analyzed using one-way Analysis of Variance models with pair-wise group differences in mean cholesterol level assessed using Tukey's post-hoc tests.

Somanathan S., Jacobs F., Wang Q., Hanlon A.L., Wilson J.M, & Rader D.J. (2014). AAV Vectors Expressing LDLR Gain-of-Function Variants Demonstrate Increased Efficacy in Mouse Models of Familial Hypercholesterolemia. Circulation research, 115(6), 591-599.

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Plasma NEFA Quantification in Equines

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Plasma NEFA concentrations were measured in mares and foals in duplicate with an enzymatic-colorimetric method using a Cobas Mira-analyzer with a commercial kit (Roche, Mannheim, Germany) as previously described [8 (link)]. The minimum level of detection was 10 μmol/L. Intra- and inter-assay coefficients of variation were 2.7% and 4.5%, respectively.

Peugnet P., Robles M., Mendoza L., Wimel L., Dubois C., Dahirel M., Guillaume D., Camous S., Berthelot V., Toquet M.P., Richard E., Sandersen C., Chaffaux S., Lejeune J.P., Tarrade A., Serteyn D, & Chavatte-Palmer P. (2015). Effects of Moderate Amounts of Barley in Late Pregnancy on Growth, Glucose Metabolism and Osteoarticular Status of Pre-Weaning Horses. PLoS ONE, 10(4), e0122596.

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Equine Metabolite Measurement Protocols

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Plasma TG, NEFA and urea concentrations were measured in mares in duplicate by an enzymatic-colorimetric method with a Cobas Mira-analyzer, using commercial kits (TG: Provet triglycerides SL, Kitvia, France; NEFA: NEFA-HR kit, Wako Chemicals GmbH, Germany; urea: Provet urea SL,Kitvia, France) and following the manufacturers’ instructions. For TG assay, intra- and inter-assay coefficients of variation were 4.6% and 7.2%, respectively. For NEFA assay, intra- and inter-assay coefficients of variation were 2.3% and 2.7%, respectively. For urea assay, intra- and inter-assay coefficients of variation were 3.0% and 6.9%, respectively.

Robles M., Nouveau E., Gautier C., Mendoza L., Dubois C., Dahirel M., Lagofun B., Aubrière M.C., Lejeune J.P., Caudron I., Guenon I., Viguié C., Wimel L., Bouraima-Lelong H., Serteyn D., Couturier-Tarrade A, & Chavatte-Palmer P. (2018). Maternal obesity increases insulin resistance, low-grade inflammation and osteochondrosis lesions in foals and yearlings until 18 months of age. PLoS ONE, 13(1), e0190309.

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Metabolic and Oxidative Profiling in Rats

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Fasting blood glucose (FBG) levels were measured by a Glucometer (Roche) at indicated time points during the study. Venous blood samples were collected after a hemodynamic study at the age of 28 weeks. Plasma glucose, glycated hemoglobin (HbA1c), creatinine, atrial natriuretic peptide (ANP), NT-proBNP, triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C), and high-density lipoprotein-cholesterol (HDL-C) were measured using Roche Diagnostics System (Madison, WI, USA). After dosing with vehicle or dapagliflozin to determine glucosuria, rats were immediately placed into metabolism cages for 24 h urine collection. Quantitative assays of urine glucose were performed by using the Cobas Mira Analyzer. Urine glucose data were normalized per 200 g body weight. A commercially available ELISA kit was used for serum 3-NT determination. The activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and the concentration of malondialdehyde (MDA) in heart homogenate was determined by commercial assay kits according to the manufacturer’s (Jiancheng Bioengineering Institute, Nanjing, China).

Feng B., Yu P., Yu H., Qian B., Li Y., Sun K., Shi B., Zhang N, & Xu G. (2023). Therapeutic effects on the development of heart failure with preserved ejection fraction by the sodium-glucose cotransporter 2 inhibitor dapagliflozin in type 2 diabetes. Diabetology & Metabolic Syndrome, 15, 141.

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