Mouse anti gfp

Manufactured by Sungene Biotech

The Mouse anti-GFP is a monoclonal antibody that specifically binds to the Green Fluorescent Protein (GFP). It can be used for the detection and quantification of GFP-tagged proteins in various applications, such as Western blotting, immunoprecipitation, and immunocytochemistry.

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Lab products found in correlation

Hrp conjugated goat anti mouse or rabbit igg, by Thermo Fisher Scientific (2 mentions) Control rabbit igg, by Merck Group (2 mentions) Control mouse igg, by Santa Cruz Biotechnology (2 mentions) Mouse anti ha, by Fortrea (2 mentions) Mouse anti flag, by Fortrea (2 mentions) Mouse anti β actin, by Fortrea (2 mentions) Anti pirf3, by Abcam (1 mentions) Anti ubiquitin, by Abcam (1 mentions) Anti tbk1, by Abcam (1 mentions) Anti p iκbα, by Abcam (1 mentions) Anti irf3, by Abcam (1 mentions) Anti usp13, by Abcam (1 mentions) Anti sting, by Abcam (1 mentions) Anti iκbα, by Abcam (1 mentions) Goat anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions) Ez link sulfo nhs lc biotin, by Thermo Fisher Scientific (1 mentions) Rabbit anti na k atpase, by Abcam (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Mouse anti β actin, by Transgene (1 mentions) Anti flag affinity gel, by Selleck Chemicals (1 mentions)

3 protocols using mouse anti gfp

Western Blotting Antibody Reagents

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Mouse control IgG (Santa Cruz Biotechnology, sc-2025) and rabbit control IgG (Millipore, 12–370), HRP-conjugated goat-anti mouse or rabbit IgG (Thermo Scientific, PA1-86717 and SA1-9510)(1:3,000), mouse anti-GFP (Sungene Biotech, KM8009)(1:1,000), mouse anti-FLAG (KM8002)(1:2,000), mouse anti-β-Actin (KM9001)(1:1,000), mouse anti-HA (COVANCE, MMS-101 R)(1:2,000), anti-pIκBα (9246L)(1:1,000), anti-Ubiquitin (sc-8017)(1:1,000), anti-IRF3 (sc-9082)(1:500), anti-IκBα (sc-371)(1:500), anti-p-IRF3 (4947 S)(1:1,000), anti-USP13 (abcam, GR56969-12)(1:500), anti-TBK1 (GR96328-11)(1:1,000) and anti-STING (13647 S)(1:1,000) were purchased from the indicated manufactures. Poly(I:C), ISD45, DNA90 and HSV120 were previously described36 (link)37 (link)38 (link)39 (link). ISD45: 5′-TACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACA-3′; DNA90: 5′-TACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACATACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACA-3′; HSV120: 5′-AGACGGTATATTTTTGCGTTATCACTGTCCCGGATTGGACACGGTCTTGTGGGATAGGCATGCCCAGAAGGCATATTGGGTTAACCCCTTTTTATTTGTGGCGGGTTTTTTGGAGGACTT-3′.

Sun H., Zhang Q., Jing Y.Y., Zhang M., Wang H.Y., Cai Z., Liuyu T., Zhang Z.D., Xiong T.C., Wu Y., Zhu Q.Y., Yao J., Shu H.B., Lin D, & Zhong B. (2017). USP13 negatively regulates antiviral responses by deubiquitinating STING. Nature Communications, 8, 15534.

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Biochemical Reagents for Cell Signaling

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Rabbit anti-TRPV1 (Alomone laboratory, catalog no. ACC-030), mouse anti-Kvβ1 (Santa Cruz, catalog no. sc-373986), rabbit anti-Na⁺/K⁺ ATPase (Abcam, catalog no. ab76020), mouse anti-GST (Abclonal, catalog no. AE001), mouse anti-β-actin (Transgen Biotech, catalog no. 6609-1), mouse anti-FLAG (Sigma, catalog no. F3165), mouse anti-FLAG (MBL, catalog no. M185-3L), rabbit anti-FLAG (Proteintech, catalog no. 20543-1-AP), mouse anti-HA (MBL, catalog no. M180-3), rabbit anti-HA (Earthox, catalog no. E02218002), mouse anti-GFP (Tianjin Sungene Biotech, catalog no. KM8009), rabbit anti-GFP (WB: Solarbio, catalog no. B1025F), anti-FLAG affinity gel (Bimake, catalog no. B23100), anti-HA affinity gel (Bimake, catalog no. B23301), ProteinIso GST resin (Transgen Biotech, catalog no. DP201-01), goat anti-mouse IgG (H + L) (Jackson Immunoresearch, catalog no. 115-035-003), and goat anti-rabbit IgG (H + L) (Jackson Immunoresearch, catalog no. 111-005-003) were purchased from the indicated manufactures. The EZ-Link Sulfo-NHS-LC-Biotin (catalog no. 21335) and high-capacity NeutrAvidin-agarose (catalog no. 29202) were ordered from Thermo Fisher Scientific.

Wang Y., Mo X., Ping C., Huang Q., Zhang H., Xie C., Zhong B., Li D, & Yao J. (2020). Site-specific contacts enable distinct modes of TRPV1 regulation by the potassium channel Kvβ1 subunit. The Journal of Biological Chemistry, 295(50), 17337-17348.

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Immunoblotting for Protein Analysis

Cited 1 time

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Mouse control IgG (Santa Cruz Biotechnology, sc-2025) and rabbit control IgG (Millipore, 12–370), HRP-conjugated goat-anti mouse or rabbit IgG (Thermo Scientific, PA1-86717 and SA1-9510) (1:3000), mouse anti-GFP (Sungene Biotech, KM8009) (1:2000), mouse anti-FLAG (KM8002) (1:2000), mouse anti-β-Actin (KM9001) (1:2000), mouse anti-HA (COVANCE, MMS-101R) (1:2000), anti-pIκBα (9246 L)(1:1000), anti-Ubiquitin (sc-8017) (1:500), anti-Ubiquitin, K48 specific (Merck, 05-1307), anti-IRF3 (sc-9082) (1:1000), anti-IκBα (sc-371) (1:1000), anti-p-IRF3 (4947 S) (1:1000), anti-TBK1 (GR96328-11) (1:2000) and anti-cGAS (sc-515777) (1:1000)were purchased from the indicated manufactures. LPS (Sigma, L2630) and CpG-B (Invivogen, tlr-1826) were purchased from the indicated manufactures. Poly(I:C), ISD45, DNA90, and HSV120 were previously described.^{35 (link)} ISD45: 5′-TACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACA-3′; DNA90: 5′-TACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACATACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACA-3′; HSV120: 5′-AGACGGTATATTTTTGCGTTATCACTGTCCCGGATTGGACACGGTCTTGTGGGATAGGCATGCCCAGAAGGCATATTGGGTTAACCCCTTTTTATTTGTGGCGGGTTTTTTGGAGGACTT-3′.

Zhang Q., Tang Z., An R., Ye L, & Zhong B. (2020). USP29 maintains the stability of cGAS and promotes cellular antiviral responses and autoimmunity. Cell Research, 30(10), 914-927.

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