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Anti b raf

Manufactured by Cell Signaling Technology
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Anti-B-Raf is a laboratory antibody product. It is used to detect and analyze the B-Raf protein, which is involved in cellular signaling pathways.

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Lab products found in correlation

Anti 4e bp1, by Cell Signaling Technology (2 mentions) Anti phospho braf, by Cell Signaling Technology (1 mentions) Anti erk, by Cell Signaling Technology (1 mentions) Phospho akt thr308 rabbit mab, by Cell Signaling Technology (1 mentions) Anti phospho erk, by Cell Signaling Technology (1 mentions) Phospho 4ebp1, by Cell Signaling Technology (1 mentions) Hvin 1, by Merck Group (1 mentions) Anti dusp4, by Cell Signaling Technology (1 mentions) Anti vinculin, by Merck Group (1 mentions) Anti p70s6k, by Cell Signaling Technology (1 mentions) Anti phospho mek1 2 ser217 221, by Cell Signaling Technology (1 mentions) Anti mnk1, by Cell Signaling Technology (1 mentions) Ly3214996, by Selleck Chemicals (1 mentions) Anti phospho 4e bp1 thr37 46, by Cell Signaling Technology (1 mentions) Anti cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions) Anti eif4e, by Cell Signaling Technology (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Anti phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Ko 947, by Selleck Chemicals (1 mentions) Anti phospho p70s6k thr421 ser424, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

B-Raf (21 citations) Anti-RAF (5 citations)

5 protocols using anti b raf

1

Western Blot Assay of Signaling Pathways

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The Western blots were carried out according to the standard protocol. The following primary antibodies were used: phospho‐Akt (Thr308) rabbit mAb (13038), phospho‐Akt rabbit mAb (Ser 473) (4060), anti‐Akt (pan) rabbit mAb (4691), phospho‐P70s6k rabbit mAb (9205), P70s6k rabbit mAb (2708), phospho‐4ebp1 (#13396, monoclonal), anti‐4ebp1 (#9644), anti‐phospho‐B‐Raf (#2696, polyclonal), anti‐B‐Raf (#9433, monoclonal), anti‐phospho‐Erk (#4370, monoclonal), anti‐Erk (#4695, monoclonal) (all from Cell Signaling Technology, Beverly, MA, USA). The band density was quantified using ImageJ software (NIH, Rockville, MD, USA).
Chang F., Zhang Y., Mi J., Zhou Q., Bai F., Xu X., Fisher D.E., Sun Q, & Wu X. (2018). ROCK inhibitor enhances the growth and migration of BRAF‐mutant skin melanoma cells. Cancer Science, 109(11), 3428-3437.
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2

Immunoblot Analysis of MAPK Pathway

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A375‐Cas9, IGR1‐Cas9, and WM983B‐Cas9 cells were transduced with lentiviral sgRNA expression vectors and cell lysates were prepared for immunoblot analysis as described previously (Christodoulou et al., 2019). Antibodies used for detection included anti‐DUSP4 (1:1,000), anti‐PPP2R2A (1:1,000), anti‐BRAF (1:1,000) (Cell Signaling Technology), and anti‐Vinculin (1:1,000, clone hVIN‐1, Sigma‐Aldrich) as a loading control. Secondary anti‐mouse and anti‐rabbit antibodies were used at 1:10,000 (Jackson ImmunoResearch Europe).
Christodoulou E., Rashid M., Pacini C., Droop A., Robertson H., van Groningen T., Teunisse A.F., Iorio F., Jochemsen A.G., Adams D.J, & van Doorn R. (2020). Analysis of CRISPR‐Cas9 screens identifies genetic dependencies in melanoma. Pigment Cell & Melanoma Research, 34(1), 122-131.
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3

Bilirubin and MAPK Pathway Inhibitors

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Bilirubin (B4126-5G) was purchased from Sigma-Aldrich (USA). Vemurafenib (S1267), KO-947 (S8569), and LY3214996 (S8534) were obtained from Selleckchem (Houston, TX). Antibodies, including anti-PARP (#9532), Caspase 3 (#9662), anti-cleaved Caspase-3 (Asp175) (#9661), anti-Caspase 9 (#9502), anti-cleaved Caspase-9 (Asp315) (#9505), anti-phospho-B-Raf (Ser445) (#2696), anti-B-Raf (#14814), anti-phospho-MEK1/2 (Ser217/221) (#3958), anti-MEK1/2 (#4694), anti-phospho-ERK1/2 (Thr202/Tyr204) (#4370), anti-ERK1/2 (#4695), anti-phospho-MNK1 (Thr197/202) (#2111), anti-MNK1 (#2195), anti-phospho-eIF4E (Ser209) (#9741), anti-eIF4E (#2067), anti-phospho-p70S6K (Thr421/Ser424) (#9204), anti-p70S6K (#2708), anti-phospho-4eBP1(Thr37/46) (#2855), anti-4eBP1 (#9644), and anti-GAPDH (#5174) were purchased from Cell Signaling Technology (Beverly, MA).
Tan Y., Zhong X., Wen X., Yao L., Shao Z., Sun W., Wu J., Wen G., Tang D., Zhang X., Liao Y, & Liu J. (2021). Bilirubin Restrains the Anticancer Effect of Vemurafenib on BRAF-Mutant Melanoma Cells Through ERK-MNK1 Signaling. Frontiers in Oncology, 11, 698888.
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4

Pancreatic Marker Antibody Validation

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Pancreatic marker Kit (Catalogue #8679; anti-α-amylase, anti-keratin, anti-PLA-2GB), anti-pERK, anti-phos-AKT, anti-BRAF, anti-Bcl2, anti-Ki67 and anti-β-actin were purchased from Cell Signaling technology (Danvers, MA) whereas anti-KRAS-GTP antibody was procured from New East Biosciences (Malvern, PA).
Ganaie A.A., Siddique H.R., Sheikh I.A., Parray A., Wang L., Panyam J., Villalta P.W., Deng Y., Konety B.R, & Saleem M. (2020). A novel terpenoid class for prevention and treatment of KRAS-driven cancers: Comprehensive analysis using in situ, in vitro and in vivo model systems. Molecular carcinogenesis, 59(8), 886-896.
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5

Antibody-based protein expression analysis

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Antibodies used were anti-PLCε, anti-PPARβ (santa cruz); anti-PPARα, anti-PPARγ, anti-Twist1, anti- phosphorylated (p)-Twist1 (Ser68), anti-PGC-1α, anti- CPT1B, anti-ERRα, anti-UCP-1, anti-ACADM, anti-MEKK3, anti-MEK4, anti-MEKK2, anti-ERK1/2, anti-p-MEK (S218/S222), anti-MEK, anti-p-JNK (Thr183/Tyr185), anti-JNK (Abcam); anti-p-ERK1/2 (Thr202/Tyr204), anti-p-P38, anti-P38, anti-B-Raf, N-Cadherin, E-Cadherin, Vimentin (Cell Signaling Technology); anti-Ras, anti-H3(wanlei); β-actin(Immunoway biotechnology); Anti-DDK (FLAG) (ORIGENE). The total protein, cell cytoplasmic protein and nuclear protein extracts and western blot assays were performed with the above antibodies by standard protocols we described before [22 (link)].
Fan J., Fan Y., Wang X., Niu L., Duan L., Yang J., Li L., Gao Y., Wu X, & Luo C. (2019). PLCε regulates prostate cancer mitochondrial oxidative metabolism and migration via upregulation of Twist1. Journal of Experimental & Clinical Cancer Research : CR, 38, 337.
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