Anti eos pe

Manufactured by Thermo Fisher Scientific

The Anti-Eos-PE is a fluorescent-labeled antibody used to detect and quantify eosinophils in biological samples. It is designed for flow cytometry applications.

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2 protocols using anti eos pe

Multi-parameter Flow Cytometry Analysis

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Flow cytometric analysis was performed using an LSR II flow cytometer (BD). Cell phenotypes were analyzed using combinations of anti-GITR-PE or -efluor450, anti-PD-1-PE, anti-FoxP3-PE, -efluor450 or -APC, anti-CD8-PE, anti-C45.1 PECy7, anti-Helios-FITC, anti-Eos-PE, anti-CD103-APC, anti-CD4-AlexaFluor700 (all from eBioscience), and anti-Neuropilin-1-PE or -APC, anti-CD103-PerCPCy5.5 (Biolegend) antibodies. Fixable viability dye efluor780 (eBioscience) was used in all experiments to exclude dead cells. Cell proliferation dye (CPD)-efluor450 and CFSE (both from eBioscience) were used to monitor cell proliferation. Anti-Ki67-PE (eBioscience) was used to identify dividing cells. Results were analyzed using FlowJo analysis software (Tree Star, Inc.).

Verhagen J., Gabryšová L., Shepard E.R, & Wraith D.C. (2014). CTLA-4 Modulates the Differentiation of Inducible Foxp3+ Treg Cells but IL-10 Mediates Their Function in Experimental Autoimmune Encephalomyelitis. PLoS ONE, 9(9), e108023.

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Multiparametric Immunophenotyping of Immune Cells

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The anti-mouse antibodies, anti-TER119-FITC, anti-CD4 FITC, anti-CD24-PE, anti-CD44-PE-Cy7, and anti-CD25-APCCy7, were purchased from eBioscience (San Diego, CA, USA). Anti-CD8-FITC, anti-CD8-AF647, and anti-CD4-Pacific Blue were purchased from BD Biosciences (San Jose, CA, USA). The anti-human antibodies, anti-CD1a-PerCP-Cy5.5, anti-CD1a-PECy5, anti-CD3-APCCy7, anti-CD4-Pacific Blue, anti-CD7-FITC, anti-CD8-BV785, Anti-CD8-FITC, anti-CD34-PE, anti-CD34-PECy7, and anti-CD38-AF700 were purchased from Biolegend (San Diego, CA). Anti-Aiolos-PE, anti-Eos-PE, and anti-Helios-AF647 were purchased from e-Bioscience. Anti-Mouse IgG1κ-PE and anti-Ikaros-PE were purchased from BD Biosciences. The anti-Armenian Hamster IgG-AF647 control was purchased from Biolegend.

Mitchell J.L., Seng A, & Yankee T.M. (2017). Expression and splicing of Ikaros family members in murine and human thymocytes. Molecular immunology, 87, 1-11.

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