Transwell chambers with 8 μm polycarbonate filters (BD Bioscience, San Diego, CA, USA) were applied for cell invasion assessment. In the procedure, chambers were coated with Matrigel on the lower side. Then, approximately 2 × 105 T24 or UM-UC-3 cells were resuspended with FBS-free medium and were planted in the upper chamber with 600 μl medium supplemented with 20% FBS in the lower chamber. Following 48 hours of incubation at 37°C, cells in the top of the membranes were removed using cotton buds and cells at the bottom of the membrane were fixed and stained with 0.2% crystal violet (Solarbio, Beijing, China). The stained cells were counted under a light microscope (magnification: 200x) to assess cell invasiveness.
Transwell chambers with 8 μm polycarbonate filters
Manufactured by BD
Sourced in United States
Transwell chambers with 8 μm polycarbonate filters are a type of cell culture insert. They consist of a porous membrane that separates the upper and lower chambers, allowing for the study of cell migration, invasion, and other cell-based assays.
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Lab products found in correlation
3 protocols using transwell chambers with 8 μm polycarbonate filters
1
Transwell Invasion Assay for Cancer Cells
2
Transwell Invasion Assay for Cancer Cells
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Transwell chambers with 8-μm polycarbonate filters (BD Bioscience, San Diego, CA, U.S.A.) were applied for cell invasion assessment. In procedure, chambers were coated with Matrigel on the lower side. Then, approximately 2 × 105 of A549 or H1975 cells resuspended with FBS-free medium were seeded in the upper chamber, while 600 μl medium supplemented with 20% FBS were added into the lower chamber. Following 48 h of incubation at 37°C with 5% CO2 or 60% H2, cells in the top of the membranes were removed using cotton buds and cells at the bottom of the membrane were fixed and stained with 0.2% Crystal Violet (Solarbio, Beijing, China). The stained cells were counted under a light microscope (magnification: 200×) to assess cell invasiveness.
3
Cell Invasion Assay Using Transwell
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Transwell chambers with 8‐μm polycarbonate filters (BD Bioscience) were applied for cell invasion assessment. In procedure, chambers were coated with Matrigel on the lower side. Then, approximately 2 × 105 HT‐1376 and T24 cells resuspended with FBS‐free medium were seeded in the upper chamber, while 600 μL medium supplemented with 20% FBS was added into the lower chamber. Following 48 hours of incubation at 37°C with 5% CO2 or 60% H2, cells in the top of the membranes were removed using cotton buds and cells at the bottom of the membrane were fixed and stained with 0.2% crystal violet (Solarbio). The stained cells were counted under a light microscope (magnification: 200×) to assess cell invasiveness.
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