Asnao2

All procedures involving mice were approved by the University of North Carolina (UNC) Institutional Animal Care and Use Committee. Male and female C57BL/6J WT mice were obtained from Jackson Laboratories (Bar Harbor, ME, USA) and let to acclimatize at the UNC Animal Facilities for one week. Male and female As3mt-KO mice on a C57BL/6 background (Drobná, et al. 2009 (link)) were bred at the UNC Animal Facilities. Both WT and As3mt-KO mice were housed under controlled conditions with 12-h light/dark cycle at 22±1°C and 50±10% relative humidity (3–5 mice per cage) and with unlimited access to pelleted 2920X Teklad rodent chow (Envigo, Madison, WI, USA). All mice were 21-week old at the beginning of the study when they were randomly assigned to treatment groups with 10–14 animals per group. Both WT and As3mt-KO mice drank for 24 weeks (ad libitum) either deionized water or deionized water containing sodium arsenite (AsNaO₂, ≥99% pure; Sigma-Aldrich, St. Louis, MO, USA) at final concentrations of 0.1 or 1.0 mg As/L (i.e., 0.1 or 1 ppm). Water with sodium arsenite was prepared weekly to minimize oxidation of iAs^III to iAs^V. Food and water consumption and body weight were monitored in all treatment groups weekly.

All procedures involving mice were approved by the University of North Carolina (UNC) Institutional Animal Care and Use Committee. Male and female C57BL6 WT mice were obtained from Jackson Laboratories (Bar Harbor, ME) and let to acclimatize at the UNC Animal Facilities for one week. These WT mice were 17 weeks (±3 days) old at the beginning of the study. Male and female As3mt-KO mice on a C57BL6 background (Drobna, et al. 2009 (link)) were bred at the UNC Animal Facilities and were 17–20 weeks old at the beginning of the study. All mice were housed under controlled conditions (12-h light/dark cycle at 22+−1°C and 50±10% relative humidity) with ad libitum access to pelleted rodent chow (Prolab Isopro RMH 3000, LabDiet, St. Louis, MO). Mice drank either deionized water or deionized water containing 1 ppm As as sodium arsenite (AsNaO₂, ≥99% pure; Sigma-Aldrich, St. Louis, MO, USA) ad libitum for four weeks (N=15–21 per experimental group). Water with iAs^III was freshly prepared every week to minimize oxidation of iAs^III to iAs^V. Food and water consumption and body weight were monitored in all treatment groups every week and two weeks, respectively.