G12m and G12msi were incubated with H4522 serum (Human AB serum, Sigma-Aldrich, St. Louis, MO, USA) at a final concentration of 50%. Samples were incubated at 37 °C, and aliquots were drawn at 0, 3, 6, 12, 24, 48, and 72 h after application. At each time point, a sample was taken and quick-frozen by placement in a deep freezer. Prior to analysis, aliquots were extracted using the phenol-chloroform-isoamyl alcohol (PCI) solution. Samples were analyzed by using denaturing polyacrylamide gel electrophoresis (PAGE) analysis. Aptamers were stained with SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen, Carlsbad, CA, USA). The stained aptamer was imaged with a LI-COR Odyssey® scanner and Image StudioTM v5.2 software (LI-COR Biotechnology, Lincoln, NE, USA) and quantified using the Odyssey software as per the manufactur’s guidelines.
H4522 serum human ab serum
Manufactured by Merck Group
Sourced in United States
H4522 serum is a laboratory product sourced from Human AB serum. It serves as a supplementary component for cell culture media.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using h4522 serum human ab serum
1
Quantifying Aptamer Stability in Human Serum
2
Aptamer Stability in Serum Incubation
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AS1411 and APTA-12 were incubated with H4522 serum (Human AB serum, Sigma-Aldrich, St. Louis, MO, USA) at a final concentration of 50% and incubated at 37°C for 0, 1, 2, 4, 8, and 24 hr. At each time point, a sample was taken and quick-frozen by placement in a deep freezer. Prior to analysis, samples were quickly thawed at 37°C and diluted with water and PCI (phenol-chloroform-isoamyl alcohol) solution. The mixtures were centrifuged at 10,000 × g for 15 min, and supernatants were used for PAGE analysis. Gels were stained with 0.5% methylene blue solution to reveal the oligonucleotide aptamers.
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