1 2 distearoyl sn glycero 3 phosphocholine

The compounds 1-palmitoyl-2-oleoyl-glycero-3phosphocholine (POPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) were purchased from NOF corporation (White Plains, NY, USA). The compound 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[azido(polyethylene glycol)-2000] (DSPE-PEG2k) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Lipopolysaccharide (LPS) (E. coli O111:B4) was purchased from Sigma Aldrich (St. Louis, MO, USA). Cholesterol was purchased from Sigma Aldrich (Milwaukee, WI, USA). Wako Cholesterol E Kit was purchased from Fujifilm (Richmond, VA, USA). IL-6 and TNF-α ELISA kits were purchased from Invitrogen (Ann Arbor, MI, USA).

1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-distearoyl-sn-glycero-3-phosphoglycerol (DSPG), and N-(carbonyl-methoxypolyethyleneglycol 2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE-PEG2000) were purchased from NOF Corporation (Tokyo, Japan). Perfluoropropane was purchased from Takachiho Chemical Industrial Co., Ltd., (Tokyo, Japan).

1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dioleoyl-sn-glycero-3-phosphoEthanolamine (DOPE), 1,2-dioleoyl-3-dimethylammonium-propane (DODAP), and 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2K) were purchased from the NOF Corporation (Tokyo, Japan). Cholesterol was purchased from Sigma-Aldrich (St. Louis, MO, USA). A pH-sensitive cationic lipid, CL4H6, was synthesized in the laboratory as previously described.^{19 (link)} Ethanol, sodium chloride, citric acid, 2-morpholinoethanesulfonic acid (MES), monohydrate, and phosphate-buffered saline (PBS) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). The siFVII and Cy-5-labeled siGL4 were purchased from Hokkaido System Science Co. Ltd. (Sapporo, Japan). Table S4† shows the sense and antisense strand sequences of siFVII and Cy5-siGL4. Quant-iT™ Ribogreen™ RNA reagent and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) were obtained from Thermo Fisher Scientific (Waltman, MA, USA). SU-8 3050 was obtained from Nippon Kayaku Co., Ltd. (Tokyo, Japan).