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Enhanced alkaline phosphatase red detection kit

Manufactured by Roche
Sourced in United States

The Enhanced Alkaline Phosphatase Red Detection Kit is a laboratory reagent used to detect the presence and activity of the enzyme alkaline phosphatase in various biological samples. It employs a colorimetric detection method that generates a red signal upon reaction with alkaline phosphatase.

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3 protocols using enhanced alkaline phosphatase red detection kit

1

Immunohistochemical Detection of Pan-TRK

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Anti-Pan-TRK antibody (Abcam; [EPR17341]) immunohistochemistry was performed on 4 μm sections obtained from formalin-fixed paraffin-embedded (FFPE) tissues on a Ventana BenchMark ULTRA system (Ventana, Tucson, USA) with the Enhanced Alkaline Phosphatase Red Detection Kit (Ventana; #800–031).
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2

Immunohistochemical Detection of Pan-TRK

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Anti-Pan-TRK antibody (Abcam; [EPR17341]) immunohistochemistry was performed on 4 μm sections obtained from formalin-fixed paraffin-embedded (FFPE) tissues on a Ventana BenchMark ULTRA system (Ventana, Tucson, USA) with the Enhanced Alkaline Phosphatase Red Detection Kit (Ventana; #800–031).
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3

Immunohistochemical Analysis of PRKAR1A, Melan A, and BRAF

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Hematoxylin-eosin and immunohistochemical staining were performed on 4 µm thick sections obtained from formalin-fixed paraffin-embedded tissues (FFPE). The Ventana BenchMark ULTRA system (Ventana, Tucson, USA) was used with the red coloring of the enhanced alkaline phosphatase red detection kit (Ventana; #800-031). The following antibodies were utilized: PRKAR1A (polyclonal rabbit, 1:200 NeoBiotech, Nanterre, France), Melan A (clone A103, 1:50, DAKO, Glostrup, Denmark), BRAF (Clone VE1, 1:100 Spring Bioscience, Pleasanton, CA).
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