The following chemicals were used: HA-tagged ubiquitin-vinyl methyl ester, HA-UbVME (Enzo Life Science, Farmingdale, NY), forskolin (FSK) (Sigma-Aldrich), USP7 inhibitor HBX41108 (Tocris, Minneapolis, MN), USP10 inhibitor spautin-1 (Cayman, Ann Arbor, MI), cycloheximide (Sigma-Aldrich). All other chemicals were purchased from Sigma-Aldrich (St Louis, MO) or EMD Millipore (Billerica, MA), unless otherwise specified. Rabbit polyclonal anti-NHE3 antibody EM450 and mouse monoclonal anti-VSVG antibody P5D4 were previously described.20 (link),21 (link) The following commercial antibodies were used: rabbit anti-HA (Cell Signaling), mouse anti-Ub (Santa Cruz), rabbit anti-USP7 (Cell Signaling), rabbit anti-USP10 (Cell Signaling), rabbit anti-Nedd4–2 (Abcam, Cambridge, MA), mouse anti-Rab5a (Cell signaling), mouse anti-Rab7 (Santa Cruz), rabbit anti-VSVG (Sigma), and mouse anti-β-actin (Sigma).
Rabbit anti usp7
Manufactured by Cell Signaling Technology
Rabbit anti-USP7 is a primary antibody that recognizes the USP7 (also known as HAUSP) protein. USP7 is a deubiquitinating enzyme that regulates the stability of various cellular proteins, including p53. This antibody can be used for applications such as Western blotting, immunoprecipitation, and immunohistochemistry to detect and study the USP7 protein.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti vsv g, by Merck Group (1 mentions)
Mouse anti ub, by Santa Cruz Biotechnology (1 mentions)
Ha ubvme, by Enzo Life Sciences (1 mentions)
Rabbit anti nedd4 2, by Abcam (1 mentions)
Mouse anti rab7, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti ha, by Cell Signaling Technology (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Forskolin fsk, by Merck Group (1 mentions)
Enhanced chemiluminescence detection kit, by Beyotime (1 mentions)
Mouse anti β actin, by Beyotime (1 mentions)
2 protocols using rabbit anti usp7
1
Ubiquitin-Mediated Protein Regulation
2
Western Blot Analysis of USP7 and p53
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Cells were harvested at indicated time and lysed by using RIPA lysis buffer on ice. A total of 20 μg protein was loaded on 7%−15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The proteins were transferred to polyvinylidene difluoride membranes and incubated with the primary antibodies rabbit anti-USP7 (#4833, Cell Signaling Technology, CST, 1:1000), rabbit anti-p53 (#2527, CST, 1:1000), mouse anti-β-actin (#AA128, Beyotime Biotech., 1:3000) at 4 °C overnight. Then membranes were washed and incubated with corresponding secondary antibodies (Beyotime). Finally, the protein was visualized using an enhanced chemiluminescence detection kit (Beyotime). The density of the bands was analyzed, and the relative ratios of target genes and references were calculated.
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