The PCR primers introduce the full-length adaptor sequence required for Illumina sequencing (for details see Illumina small RNA PCR primers). PCR was performed in 12.5μl using half of the ranhexRT sample as a template [1X KAPA HiFi HotStart ReadyMix (KapaBiosystems #KK2602), 400 nM each primer]. The final PCR amplified library was submitted to two consecutive 1x NucleoMag NGS Clean-up and Size select magnetic beads (Macherey-Nagel #7449970.5) according to manufacturer’s instructions. The final library was eluted in 20μl of 10 mM Trizma hydrochloride solution (Sigma-Aldrich #T2319–1L).
Small rna pcr primers
Manufactured by Illumina
Small RNA PCR primers are laboratory equipment designed to amplify small RNA molecules, such as microRNAs, through the process of Polymerase Chain Reaction (PCR). These primers are specific sequences of nucleic acids that bind to the target small RNA, allowing for its selective amplification and detection.
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2 protocols using small rna pcr primers
1
Illumina Sequencing Library Preparation
2
Illumina Small RNA Library Preparation
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The PCR primers introduce the full-length adaptor sequence required for Illumina sequencing (for details see Illumina small RNA PCR primers). PCR was performed in 12.5μL using half of the ranhexRT sample as a template (1X KAPA HiFi HotStart ReadyMix (KapaBiosystems KK2602), 400 nM each primer). The final PCR amplified Library was submitted to two consecutive 1x NucleoMag NGS Clean-up and Size select magnetic beads (Macherey-Nagel, 7449970.5) according to manufacturer’s instruction. The final library was eluted in 20μL of 10 mM Trizma hydrochlorid solution (Sigma-Aldrich, T2319-1L).
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