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2 protocols using quercetin 3.4 di o glucoside

1

Antioxidant Compound Characterization Protocol

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2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) were purchased from Sigma Aldrich (Schnelldorf, Germany). Methanol (LC-MS grade) was purchased from Chem-Lab (Zedelgem, Belgium). Formic acid (LC-MS grade) was purchased from Fischer Scientific (Madrid, Spain); Trolox was purchased from Acrὸs organics (Morris, NJ, USA). Water (LC/MS grade) was purified using a Millipore Direct-Q 3UV apparatus (18.2 mΩ*sec). Standard compounds (catechin, procyanidin B1, epicatechin, chlorogenic acid, myricetin glucoside, kaempferol glucoside, quercitrin, quercetin 3.4-di-O-glucoside, quercetin, kaempferol, rutin, amentoflavone, hypericin) were purchased from ExtraSynthese (Genay, France) and hyperforin was purchased from ChromaDex (Los Angeles, CA, USA).
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2

Dietary Iron Absorption Study

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Low iron diet was obtained by Special Diet Service, UK. Pentobarbitone sodium (Pentoject) was obtained from Animalcare Ltd., UK. 59Fe was obtained by PerkinElmer, USA. Quercetin-3,4′-di-O-glucoside, quercetin-3-O-glucuronide, isorhamnetin-3-O-glucoside and isorhamnetin were obtained by Extrasynthese, France. All other chemicals were purchased from Sigma-Aldrich, USA.
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