K375 100

Manufactured by Abcam

Sourced in United States

K375-100 is an antibody product sold by Abcam. It is a 100 test size.

Automatically generated - may contain errors

Lab products found in correlation

K625 100, by Abcam (2 mentions) Human albumin elisa quantification kit, by Fortis Life Sciences (1 mentions) Amplex red, by Thermo Fisher Scientific (1 mentions) Qubit fluorometer, by Thermo Fisher Scientific (1 mentions) Eia kit, by Cayman Chemical (1 mentions)

4 protocols using k375 100

Plasma and Urine Creatinine and Urea Assay

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The concentrations of creatinine in the plasma and urine were assayed using the kit (K625-100, BioVision, California, USA). The concentration of urea in the plasma was determined using the kit (K375-100, BioVision, California, USA).

Yu S.Q., Ma S, & Wang D.H. (2020). TRPV1 Activation Prevents Renal Ischemia-Reperfusion Injury-Induced Increase in Salt Sensitivity by Suppressing Renal Sympathetic Nerve  Activity. Current Hypertension Reviews, 16(2), 148-155.

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Quantifying Albumin and Urea Secretion in Spheroids and Organoids

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To determine the levels of albumin secreted over time by spheroids and organoids, co-culture media were collected from perfusion channels and graft chambers (day 3, 5, and 7 for spheroids, day 7, 14, and 21 for organoids) and analyzed using the Human Albumin ELISA Quantification kit (Bethyl Laboratories Inc., E80-129, sample dilution 1:125).
Urea production was determined by a colorimetric assay kit (Biovision, #K375-100, sample dilution 1:5) following the manufacturer’s protocol.

Bonanini F., Kurek D., Previdi S., Nicolas A., Hendriks D., de Ruiter S., Meyer M., Clapés Cabrer M., Dinkelberg R., García S.B., Kramer B., Olivier T., Hu H., López-Iglesias C., Schavemaker F., Walinga E., Dutta D., Queiroz K., Domansky K., Ronden B., Joore J., Lanz H.L., Peters P.J., Trietsch S.J., Clevers H, & Vulto P. (2022). In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed. Angiogenesis, 25(4), 455-470.

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Metabolite Profiling of Hepatocyte Supernatants

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Levels of glucose, glutamic acid and urate from supernatants were measured using AmplexRed-based assays (Invitrogen: A22189, A12221 and A22181) according to manufacturer's directions for the Qubit fluorometer. Urea levels were measured using a colorimetric assay kit (Biovision, K375-100) according to manufacturer's directions. Following the assays, the background level of each measured metabolite in fresh WEM or WEM with theophylline was averaged (n=2) and subtracted from the individual respective supernatant samples. Endpoint concentrations were determined by adding back the average baseline concentration of fresh media without theophylline. Endpoint concentration changes between theophylline (n=4) and control treated samples (n=4) were determined for each metabolite using an unpaired two-sided Student's t-test. Statistical significance was determined independently for rat and human hepatocyte experiments by correcting for multiple hypothesis testing across the four metabolites and applying a FDR-adjusted q-value threshold of 0.05.

Blais E.M., Rawls K.D., Dougherty B.V., Li Z.I., Kolling G.L., Ye P., Wallqvist A, & Papin J.A. (2017). Reconciled rat and human metabolic networks for comparative toxicogenomics and biomarker predictions. Nature Communications, 8, 14250.

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Creatinine and Urea Quantification

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Plasma and urine creatinine concentrations were measured using a kit (K625–100, BioVision, Mountain View, CA, USA). Plasma concentrations of urea were determined using a kit (K375–100, BioVision). Urinary 8-isoprostane levels were determined using an EIA kit (516351, Cayman Chemical, Ann Arbor, MA, USA).

Yu S.Q., Ma S, & Wang D.H. (2020). Ablation of TRPV1-positive nerves exacerbates salt-induced hypertension and tissue injury in rats after renal ischemia-reperfusion via infiltration of macrophages. Clinical and experimental hypertension (New York, N.Y. : 1993), 43(3), 254-262.

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