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2 protocols using scintillation cocktail ultima gold xr

1

Cytotoxic Effects of Methanol and Ethidium Bromide on AGS Cells

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Methanol and ethidium bromide, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), were purchased from Sigma Chemical Co. (USA). Stock cultures of AGS-CRL-1739 human stomach cancer cells were purchased from the American Type Culture Collection (USA). Ham's F-12 K (Kaighn's) Medium and fetal bovine serum (FBS) used in a cell culture were products of Gibco (USA). Glutamine, penicillin, and streptomycin were obtained from Quality Biologicals Inc. (USA); [3H]thymidine (6.7 Ci mmol−1) was purchased from NEN (USA), and Scintillation Cocktail “Ultima Gold XR” from Packard (USA). Sodium dodecyl sulfate was received from Bio-Rad Laboratories (USA). Acridine orange and ethidium bromide were provided by Sigma Chemical Co. (USA). FITC Annexin V Apoptosis Detection Kit II was product of BD Pharmingen.
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2

Cisplatin-induced Apoptosis in Breast Cancer

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Cisplatin, echistatin, DMSO, 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT), sodium dodecyl sulfate (SDS) were provided by Sigma-Aldrich (USA). Stock culture of human MDA-MB-231 breast cancer was purchased from the American Type Culture Collection (USA). Dulbecco’s minima essential medium (DMEM) and fetal bovine serum (FBS) used in a cell culture were products of Gibco (USA). Glutamine, penicillin, and streptomycin were obtained from QualityBiological Inc. (USA). [3H]thymidine (6.7 Ci/mmol) were purchased from NEN (USA), and scintillation cocktail “Ultima GoldXR” from Packard (USA). FITC Annexin V Apoptosis Detection Kit was a product of BD Pharmigen (USA).
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